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Anti-APPL1 Polyclonal Antibody

Catalog #:   PHJ78701 Specific References (11) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9UKG1
Overview

Catalog No.

PHJ78701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APPL1 (Phe404-Ser673).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

APPL1,Dip13-alpha,APPL,DIP13A,DCC-interacting protein 13-alpha,KIAA1428,Adapter protein containing PH domain,PTB domain and leucine zipper motif 1

Purification

Purified by antigen affinity column.

Accession

Q9UKG1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with APPL1 antibody (PHJ78701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 32 kDa
    Observed MW: 32 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with APPL1 antibody (PHJ78701) at 1 μg/ml.

    Lane 1: L6
    Lane 2: NIH3T3

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 80 kDa
    Observed MW: 80 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse heart stained for APPL1 with PHJ78701.
References

Genotype, Phenotype, and Clinical Characteristics of Maturity-onset Diabetes of the Young (MODY): Predominance of GCK-MODY., PMID:40338033

Screening of Mutations in Maturity-onset Diabetes of the Young-related Genes and RFX6 in Children with Autoantibody-negative Type 1 Diabetes Mellitus., PMID:38054414

Aberrant protein expression of Appl1, Sortilin and Syndecan-1 during the biological progression of prostate cancer., PMID:36089417

Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins., PMID:34817533

Sex differences in the regulation of porcine coronary artery tone by perivascular adipose tissue: a role of adiponectin?, PMID:28593738

Neuropilin-1 (NRP-1)/GIPC1 pathway mediates glioma progression., PMID:27481513

Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients., PMID:27075719

Adiponectin Promotes Human Jaw Bone Marrow Stem Cell Osteogenesis., PMID:26961489

Netrin-1 protects hypoxia-induced mitochondrial apoptosis through HSP27 expression via DCC- and integrin α6β4-dependent Akt, GSK-3β, and HSF-1 in mesenchymal stem cells., PMID:23538444

Differential phosphorylation of the phosphoinositide 3-phosphatase MTMR2 regulates its association with early endosomal subtypes., PMID:23378027

APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction., PMID:17000777

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-APPL1 Polyclonal Antibody [PHJ78701]
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