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Anti-Human CD75/ST6GAL1 Polyclonal Antibody

Catalog #:   PHD13701 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P15907
Overview

Catalog No.

PHD13701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD75/ST6GAL1 (Lys27-Cys406).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Beta-galactoside alpha-2,6-sialyltransferase 1, Alpha 2,6-ST 1, 2.4.3.1, B-cell antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6Gal I, ST6GalI, Sialyltransferase 1, ST6GAL1, SIAT1

Purification

Purified by antigen affinity column.

Accession

P15907

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD75/ST6GAL1 antibody (PHD13701) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 46 kDa
    Observed MW: 46 kDa
References

The incorrect use of CD75 as a synonym for ST6GAL1 has fostered the expansion of commercial "ST6GAL1" antibodies that do not recognize ST6GAL1., PMID:35789385

Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting., PMID:31254446

Proteomics Profiling of CLL Versus Healthy B-cells Identifies Putative Therapeutic Targets and a Subtype-independent Signature of Spliceosome Dysregulation., PMID:29367434

Cell surface markers for the identification and study of human naive pluripotent stem cells., PMID:29227830

Characteristics of two CD75-related cell-surface expressed antigens of human lymphocytes., PMID:17067678

T-bet-positive and IRTA1-positive monocytoid B cells differ from marginal zone B cells and epithelial-associated B cells in their antigen profile and topographical distribution., PMID:16079106

Identification of a tonsil IgD+ B cell subset with phenotypical and functional characteristics of germinal center B cells., PMID:8765011

Production of two monoclonal antibodies (FB1 and FB21) useful for the identification of human B lymphocytes in formalin-fixed, paraffin-embedded tissues., PMID:7965394

The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and alpha 2-6 sialyltransferase, CD75, on B cells., PMID:1717156

Quantitation of IgM antibodies to the M. leprae synthetic disaccharide can predict early bacterial multiplication in leprosy., PMID:2205684

Characterization of a cell surface molecule expressed on B-lymphocytes and Hodgkin's cells., PMID:1689214

Antigenic expression of B-cell chronic lymphocytic leukemic lymphocytes., PMID:2803444

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CD75/ST6GAL1 Polyclonal Antibody [PHD13701]
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