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Anti-Clostridium perfringens cpb/Beta-toxin Polyclonal Antibody

Catalog #:   PXX21001 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: WP_004456321.1
Overview

Catalog No.

PXX21001

Species reactivity

Clostridium perfringens

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Clostridium perfringens cpb/Beta-toxin (Asn28-Ile336).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

beta-channel forming cytolysin CPB, cpb

Concentration

0.66 mg/ml

Purification

Purified by antigen affinity column.

Accession

WP_004456321.1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with cpb/Beta-toxin antibody (PXX21001) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

An in vitro assay for toxicity testing of Clostridium perfringens type C β-toxin., PMID:38646535

A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia., PMID:38308826

Novel toxin-based mRNA vaccine against Clostridium perfringens using in silico approaches., PMID:38185287

Immunization of sheep with a recombinant vaccine containing immunogenic nontoxic domains of Clostridium perfringens alpha and beta toxins., PMID:37516212

Platelet Endothelial Cell Adhesion Molecule 1 (CD31) Is Essential for Clostridium perfringens Beta-Toxin Mediated Cytotoxicity in Human Endothelial and Monocytic Cells., PMID:34941730

Measurement over 1 Year of Neutralizing Antibodies in Cattle Immunized with Trivalent Vaccines Recombinant Alpha, Beta and Epsilon of Clostridium perfringens., PMID:34564599

Humoral Immune Response Evaluation in Horses Vaccinated with Recombinant Clostridium perfringens Toxoids Alpha and Beta for 12 Months., PMID:34437437

Evaluation of the expression and immunogenicity of four versions of recombinant Clostridium perfringens beta toxin designed by bioinformatics tools., PMID:33508438

Immunization of mice against alpha, beta, and epsilon toxins of Clostridium perfringens using recombinant rCpa-b-x expressed by Bacillus subtilis., PMID:32447084

Vaccination against Clostridium perfringens type C enteritis in pigs: a field study using an adapted vaccination scheme., PMID:31428441

Application of an Endothelial Cell Culture Assay for the Detection of Neutralizing Anti-Clostridium Perfringens Beta-Toxin Antibodies in a Porcine Vaccination Trial., PMID:30991691

Clostridium perfringens beta toxin DNA prime-protein boost elicits enhanced protective immune response in mice., PMID:28523396

The CpAL system regulates changes of the trans-epithelial resistance of human enterocytes during Clostridium perfringens type C infection., PMID:27063897

Generation and characterization of recombinant bivalent fusion protein r-Cpib for immunotherapy against Clostridium perfringens beta and iota toxemia., PMID:26774054

Synergistic effects of Clostridium perfringens enterotoxin and beta toxin in rabbit small intestinal loops., PMID:24778117

[Occurrence and control of the Clostridium perfringens type A associated diarrhea of the suckling pigs with special consideration of the immunoprophylaxis]., PMID:23242147

Potential protective immunogenicity of recombinant Clostridium perfringens α-β2-β1 fusion toxin in mice, sows and cows., PMID:21641956

Development and application of new mouse models to study the pathogenesis of Clostridium perfringens type C Enterotoxemias., PMID:19805537

Involvement of tumour necrosis factor-alpha in Clostridium perfringens beta-toxin-induced plasma extravasation in mice., PMID:18264118

Beta toxin is essential for the intestinal virulence of Clostridium perfringens type C disease isolate CN3685 in a rabbit ileal loop model., PMID:18078439

Both epsilon-toxin and beta-toxin are important for the lethal properties of Clostridium perfringens type B isolates in the mouse intravenous injection model., PMID:17210666

Dissecting the contributions of Clostridium perfringens type C toxins to lethality in the mouse intravenous injection model., PMID:16926413

Cloning of alpha-beta fusion gene from Clostridium perfringens and its expression., PMID:16534876

Development and prevalidation of two different ELISA systems for the potency testing of Clostridium perfringens beta and epsilon-toxoid containing veterinary vaccines., PMID:10397315

Development and evaluation of various enzyme-linked immunosorbent assays for the detection of Clostridium perfringens beta anti-toxins., PMID:10397314

Measurement of Clostridium perfringens beta-toxin production by surface plasmon resonance immunoassay., PMID:9682350

Development of double sandwich ELISA for Clostridium perfringens beta and epsilon toxins., PMID:1615638

Enzyme-linked immunosorbent assay for rapid detection of toxins from Clostridium perfringens., PMID:1769554

Protection of the nursing pig against experimentally induced enteric colibacillosis by vaccination of dam with fimbrial antigens of E coli (K88, K99 and 987P)., PMID:3907113

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Clostridium perfringens cpb/Beta-toxin Polyclonal Antibody [PXX21001]
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