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Anti-PHPT1 Polyclonal Antibody

Catalog #:   PHJ66801 Specific References (6) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9NRX4
Overview

Catalog No.

PHJ66801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PHPT1 (Met1-Tyr125).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Protein histidine phosphatase,Protein janus-A homolog,PHPT1,PHP14,14 kDa phosphohistidine phosphatase,PHP,Phosphohistidine phosphatase 1

Purification

Purified by antigen affinity column.

Accession

Q9NRX4

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PHPT1 antibody (PHJ66801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
References

[Progress in enrichment methods for protein N-phosphorylation]., PMID:38966971

Histidine kinase NME1 and NME2 are involved in TGF-β1-induced HSC activation and CCl4-induced liver fibrosis., PMID:32860079

pHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification., PMID:28129587

Influence of protein histidine phosphatase overexpression and down-regulation on human umbilical-vein endothelial cell viability., PMID:21967643

Regulation of glucose- and mitochondrial fuel-induced insulin secretion by a cytosolic protein histidine phosphatase in pancreatic beta-cells., PMID:20501872

Immunohistochemical localization of phosphohistidine phosphatase PHPT1 in mouse and human tissues., PMID:19396692

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-PHPT1 Polyclonal Antibody [PHJ66801]
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