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Anti-GFP Polyclonal Antibody

Catalog #:   PWK08401 Specific References (51) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P42212
Overview

Catalog No.

PWK08401

Species reactivity

GFP tag

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant GFP (Met1-Lys238).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000

Target

Green fluorescent protein, GFP

Concentration

2.46 mg/ml

Purification

Purified by antigen affinity column.

Accession

P42212

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GFP antibody (PWK08401) at 1 µg/ml.

    Lane 1: Recombinant Enhanced GFP Protein (YWK08401)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
References

Li, Z., Xu, K., Zhou, Z., Liang, C., Gu, W., and Ran, J. (2024). A novel SOX10 mutation causing Waardenburg syndrome type 2 by expressing a truncated and dysfunctional protein in a Chinese child. Molecular Biology Reports 51, 536. PMID: 38642155

Natural Peptidoglycan Nanoparticles Enable Rapid Antigen Purification and Potent Delivery for Plant-derived Vaccines., PMID:40528346

Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector., PMID:40515423

Unraveling Botulinum Neurotoxin A Light-Chain-Induced Signaling Pathways: A Phosphoproteomic Analysis in a Controlled Cellular Model., PMID:40507977

Combining CD3/GD2 bispecific T cell engager with human Vγ9Vδ2 T cells facilitates neuroblastoma cell targeting and killing in vitro., PMID:40489497

A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins., PMID:40485297

The phosphatase activity of the PPP2R5D-PP2A holoenzyme modulates liprin-α1 liquid-liquid phase separation., PMID:40484382

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry., PMID:40483334

Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe., PMID:40479561

Patritumab deruxtecan induces immunogenic cell death., PMID:40458967

High-Efficient and Controllable Purification of Protein by Cyclic Injection in an Orthogonal Micro-Free-Flow Electrophoresis., PMID:40442025

Development and characterization of a novel NEIL1 nanobody., PMID:40440929

Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies., PMID:40419540

Methods for detection of cardiac glycogen-autophagy., PMID:40395528

The role of tungsten in modulating DNA double-strand break repair and class switch recombination., PMID:40360058

A pectin acetyl-transferase facilitates secondary plasmodesmata formation and RNA silencing movement between plant cells., PMID:40353833

Comparison of the induction of neutralizing antibodies against Bas congo virus using several vaccine modalities., PMID:40348847

A Liposome-Based Nanoparticle Vaccine Induces Effective Immunity Against EBV Infection., PMID:40338258

Protective antibody response in Korean raccoon dogs (Nyctereutes procynoide koreensis) administered a new rabies bait vaccine containing the ERAGS-GFP strain., PMID:40321792

Real-Time Affinity Measurements of Proteins Synthesized in Cell-Free Lysate Using Fluorescence Correlation Spectroscopy., PMID:40305650

Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap., PMID:40304578

TRPV1 Defends the Healthy Murine Cornea against Staphylococcus aureus Adhesion Independently of Sensory Nerve Firing., PMID:40291673

Establishment of a pseudovirus neutralization assay for TGEV., PMID:40276514

Simian Immunodeficiency Virus-Based Virus-like Particles Are an Efficient Tool to Induce Persistent Anti-SARS-CoV-2 Spike Neutralizing Antibodies and Specific T Cells in Mice., PMID:40266067

Activated blood-derived human primary T cells support replication of HAdV C5 and virus transmission to polarized human primary epithelial cells., PMID:40265914

B cells modulate lung antiviral inflammatory responses via the neurotransmitter acetylcholine., PMID:40263611

The genetic, biophysical and immunological studies of a self-adjuvanted protein nanoparticle., PMID:40262373

High-throughput optimization of peptide-linker for fusing function protein with GFP., PMID:40239936

Expression, purification, and immunogenicity study of human papillomavirus type 52 virus-like particles produced in Hansenula polymorpha., PMID:40228400

An improved cytological assay for R-loop detection in Saccharomyces cerevisiae utilizing a catalytically inactive RNase H., PMID:40205716

Rapid Isolation and Characterization of Exosomes through a Single-Step, Label-Free Protein Biomarker Analysis., PMID:40203051

Intronic RNAscope probes enable precise identification of cardiomyocyte nuclei and cell cycle activity., PMID:40195462

In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE., PMID:40194674

Lenacapavir disrupts HIV-1 core integrity while stabilizing the capsid lattice., PMID:40168125

Development and optimization of human T-cell leukemia virus-specific antibody-dependent cell-mediated cytotoxicity (ADCC) assay directed to the envelope protein., PMID:40152593

Decoding plasma cell maturation dynamics with BCMA., PMID:40134424

Tracking clusterin expression in hematopoietic stem cells reveals their heterogeneous composition across the lifespan., PMID:40132120

PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis., PMID:40128278

Comparative Analysis of SPLICS and MCS-DETECT for Detecting Mitochondria-ER Contact Sites (MERCs)., PMID:40115170

Osteopontin inhibits autophagy via CD44 and avβ3 integrin and promotes cell proliferation in osteoarthritic fibroblast-like synoviocytes., PMID:40102843

Isolation of Adult Hippocampal Neural Stem Cells., PMID:40067614

Development and Application of a Cell-Based Assay for Detecting Anti-Agrin Antibodies Associated With Myasthenia Gravis., PMID:40065451

Regulating ferredoxin electron transfer using nanobody and antigen interactions., PMID:40059882

Cleavage of the selective autophagy receptor NBR1 by the PDCoV main protease NSP5 impairs autophagic degradation of the viral envelope protein., PMID:40047225

Targets for the diagnosis of Acanthamoeba eye infections include four cyst wall proteins and the mannose-binding domain of the trophozoite mannose-binding protein., PMID:40035521

Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy., PMID:40029851

Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein., PMID:39991067

Evaluation of Immunopharmacological efficacy of live Leishmania donovani overexpressing Ld_ζ1domain for protection against experimental human visceral Leishmaniasis., PMID:39983418

Efficient Identification of Monoclonal Antibodies Against Rift Valley Fever Virus Using High-Throughput Single Lymphocyte Transcriptomics of Immunized Mice., PMID:39982227

The CD64/CD28/CD3ζ chimeric receptor reprograms T-cell metabolism and promotes T-cell persistence and immune functions while triggering antibody-independent and antibody-dependent cytotoxicity., PMID:39962623

Mechanistic insight into the induction of liver tissue-resident memory CD8+ T cells by glycolipid-peptide vaccination., PMID:39946236

Datasheet

Document Download

Anti-GFP Polyclonal Antibody.pdf

 

$ 130
Product specifications
50 μg 130 100 μg 220

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Anti-GFP Polyclonal Antibody [PWK08401]
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