Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-HSPA5 Polyclonal Antibody

Catalog #:   PHC84902 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P11021
Overview

Catalog No.

PHC84902

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA5 (Leu351-Leu654).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein

Purification

Purified by antigen affinity column.

Accession

P11021

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with HSPA5 antibody (PHC84902) at 1 µg/ml.

    Lane 1: HepG2 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: 4T1 cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: Hela cell lysate
    Lane 8: A549 cell lysate
    Lane 9: Mouse brain lysate
    Lane 10: Mouse liver lysate
    Lane 11: Rat brain lysate
    Lane 12: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 72 kDa
    Observed MW: 70-78 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA5 antibody (PHC84902) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: Human
    Observed MW:
References

Differential Induction of Endoplasmic Reticulum Stress Signaling by Antibody Isotypes: Implications for Plasma Cell Differentiation., PMID:40285383

GRP78 Nanobody-Directed Immunotoxin Activates Innate Immunity Through STING Pathway to Synergize Tumor Immunotherapy., PMID:40135833

A humanized antibody against mucormycosis targets angioinvasion and augments the host immune response., PMID:40073153

Celastrol-loaded ginsenoside Rg3 liposomes enhance anti-programmed death ligand 1 immunotherapy by inducing immunogenic cell death in triple-negative breast cancer., PMID:39986227

Design and Cytotoxicity Evaluation of a Cancer-targeting Immunotoxin Based on a Camelid Nanobody-PE Fusion Protein., PMID:39731471

Blood-Brain Barrier Disruption in Neuroimmunological Disease., PMID:39408955

In silico and in vitro evaluation of a PE38 and Nb-based recombinant immunotoxin targeting the GRP78 receptor in cancer cells., PMID:39397264

A novel bispecific T-cell engager using the ligand-target csGRP78 against acute myeloid leukemia., PMID:39196413

CD5L up-regulates the TGF-β signaling pathway and promotes renal fibrosis., PMID:39127319

UGT1A7 altered HER2-positive breast cancer response to trastuzumab by affecting epithelial-to-mesenchymal transition: A potential biomarker to identify patients resistant to trastuzumab treatment., PMID:39122832

Small molecules targeting GRP78 mitigate anti-GRP78 autoantibody-mediated tissue factor procoagulant activity in cultured endothelial cells., PMID:39111636

Cobalt(III) prodrug-based nanomedicine for inducing immunogenic cell death and enhancing chemo-immunotherapy., PMID:39033985

Immunoexpression Pattern of Autophagy-Related Proteins in Human Congenital Anomalies of the Kidney and Urinary Tract., PMID:38999938

Inhibition of ER stress using tauroursodeoxycholic acid rescues obesity-evoked cardiac remodeling and contractile anomalies through regulation of ferroptosis., PMID:38906502

Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells., PMID:38896138

The dispensability of VH-VL pairing and the indispensability of VL domain integrity in the IgG1 secretion process., PMID:38756530

Porcine epidemic diarrhea virus E protein induces formation of stress granules and attenuates protein translation through activation of the PERK/eIF2α signaling pathway., PMID:38643723

Endoplasmic Reticulum Stress and Unfolded Protein Response in Vernal Keratoconjunctivitis., PMID:38597723

Enhancing precision medicine: Bispecific antibody-mediated targeted delivery of lipid nanoparticles for potential cancer therapy., PMID:38467208

Intestinal heat shock proteins in metabolic syndrome: Novel mediators of obesity and its comorbidities resolution after metabolic surgery., PMID:38412940

SYT7 (synaptotagmin 7) promotes cervical squamous cell carcinoma., PMID:38314285

Fatal attractions that trigger inflammation and drive atherosclerotic disease., PMID:38287209

Platelets induce endoplasmic reticulum stress in macrophages in vitro., PMID:38278417

Association of COVID with Mycosis in General., PMID:38251692

Landscape of protein-protein interactions during hepatitis C virus assembly and release., PMID:38230952

Cell surface GRP78: A potential therapeutic target for high glucose-induced endothelial injury., PMID:38056158

Single-cell transcriptomic atlas throughout anti-BCMA CAR-T therapy in patients with multiple myeloma., PMID:38035111

Identification of Diagnosis and Typological Characteristics Associated with Ferroptosis for Ulcerative Colitis via Bioinformatics and Machine Learning., PMID:37957905

Direct interaction of the molecular chaperone GRP78/BiP with the Newcastle disease virus hemagglutinin-neuraminidase protein plays a vital role in viral attachment to and infection of culture cells., PMID:37920471

Association of maternal thyroid peroxidase antibody during pregnancy with placental morphology and inflammatory and oxidative stress responses., PMID:37810887

The chaperone protein GRP78 released from MPN cells increases the expression of lysyl oxidase in a human stromal cell line., PMID:37757654

Mechanistic characterization of disulfide bond reduction of an ERAD substrate mediated by cooperation between ERdj5 and BiP., PMID:37739037

Recent advances in small molecule and peptide inhibitors of glucose-regulated protein 78 for cancer therapy., PMID:37690265

Disturbance of neuron-microglia crosstalk mediated by GRP78 in Neuropsychiatric systemic lupus erythematosus mice., PMID:37365565

HSPA5 Promotes Attachment and Internalization of Porcine Epidemic Diarrhea Virus through Interaction with the Spike Protein and the Endo-/Lysosomal Pathway., PMID:37222617

Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation., PMID:37137223

Immunoexpression Pattern of Autophagy Markers in Developing and Postnatal Kidneys of Dab1-/-(yotari) Mice., PMID:36979337

An antibody that targets cell-surface glucose-regulated protein-78 inhibits expression of inflammatory cytokines and plasminogen activator inhibitors by macrophages., PMID:36947703

An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway., PMID:36860866

Salvianolic acid A attenuates inflammation-mediated atherosclerosis by suppressing GRP78 secretion of endothelial cells., PMID:36758912

Citrullinated glucose-regulated protein 78 is a candidate target for melanoma immunotherapy., PMID:36544781

Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis., PMID:36513656

Hormonal therapies up-regulate MANF and overcome female susceptibility to immune checkpoint inhibitor myocarditis., PMID:36322628

Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma., PMID:36291095

Paraneoplastic Neuromyelitis Optica Spectrum Disorder Related to Glucose-regulated Protein 78 (GRP78) Autoantibodies in a Patient with Lynch Syndrome-associated Colorectal Cancer., PMID:36288992

Pathomechanisms in demyelination and astrocytopathy: autoantibodies to AQP4, MOG, GFAP, GRP78 and beyond., PMID:35674086

Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells., PMID:35610229

Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells., PMID:35182754

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy., PMID:34943954

Macrophage-Derived Immunoglobulin M Inhibits Inflammatory Responses via Modulating Endoplasmic Reticulum Stress., PMID:34831038

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-HSPA5 Polyclonal Antibody [PHC84902]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only