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Anti-HDAC8 Polyclonal Antibody

Catalog #:   PHJ51301 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BY41
Overview

Catalog No.

PHJ51301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HDAC8 (Met1-Val377).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HDAC8,Histone deacetylase 8,HD8,HDACL1

Purification

Purified by antigen affinity column.

Accession

Q9BY41

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HDAC8 antibody (PHJ51301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 44 kDa
    Observed MW: 44 kDa
  • Immunocytochemistry/ Immunofluorescence
    HDAC8 in MDA-MB-231 Cell Line.
    The MDA-MB-231 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with HDAC8 (PHJ51301) at 7.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Unveiling the PDK4-centered rituximab-resistant mechanism in DLBCL: the potential of the "Smart" exosome nanoparticle therapy., PMID:39004737

Histone deacetylase 8 inhibition suppresses mantle cell lymphoma viability while preserving natural killer cell function., PMID:33190829

HDAC8 inhibition ameliorates pulmonary fibrosis., PMID:30358439

Cooperativity of HOXA5 and STAT3 Is Critical for HDAC8 Inhibition-Mediated Transcriptional Activation of PD-L1 in Human Melanoma Cells., PMID:29174371

Analysis of the interactome of Schistosoma mansoni histone deacetylase 8., PMID:29155817

HDAC inhibitors enhance the immunotherapy response of melanoma cells., PMID:29137331

Histone deacetylase inhibitors attenuate P-aIgA1-induced cell proliferation and extracellular matrix synthesis in human renal mesangial cells in vitro., PMID:26775659

Specific inhibition of histone deacetylase 8 reduces gene expression and production of proinflammatory cytokines in vitro and in vivo., PMID:25451941

Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells by inhibiting histone H3K9 acetylation and RUNX2 activity., PMID:25019367

Histone deacetylase 8 regulates cortactin deacetylation and contraction in smooth muscle tissues., PMID:24920679

Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells., PMID:18617272

Novel smooth muscle markers reveal abnormalities of the intestinal musculature in severe colorectal motility disorders., PMID:16771768

Screening of histone deacetylases (HDAC) expression in human prostate cancer reveals distinct class I HDAC profiles between epithelial and stromal cells., PMID:15590418

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-HDAC8 Polyclonal Antibody [PHJ51301]
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