Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-HEPH Polyclonal Antibody

Catalog #:   PHJ49001 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BQS7
Overview

Catalog No.

PHJ49001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HEPH (Ala24-Cys366).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HEPH,KIAA0698,Hephaestin

Purification

Purified by antigen affinity column.

Accession

Q9BQS7

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HEPH antibody (PHJ49001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 41 kDa
    Observed MW: 41 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with HEPH antibody (PHJ49001) at 1 μg/ml.

    Lane 1: Human serum

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 136 kDa
    Observed MW: 136 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse testis stained for HEPH with PHJ49001.
References

Immunoreactivities for hepcidin, ferroportin, and hephaestin in astrocytes and choroid plexus epithelium of human brains., PMID:31755170

An immunohistochemical study of placental syncytiotrophoblasts in neonatal hemochromatosis., PMID:27871472

Immunoreactive hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes., PMID:22350470

Interactions between ferroportin and hephaestin in rat enterocytes are reduced after iron ingestion., PMID:21473866

Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic {beta}-cells., PMID:20019163

Iron feeding induces ferroportin 1 and hephaestin migration and interaction in rat duodenal epithelium., PMID:18974313

Ceruloplasmin/hephaestin knockout mice model morphologic and molecular features of AMD., PMID:18326691

Ferroportin1 and hephaestin are involved in the nigral iron accumulation of 6-OHDA-lesioned rats., PMID:17561842

Colocalization of ferroportin-1 with hephaestin on the basolateral membrane of human intestinal absorptive cells., PMID:17486601

Systemic regulation of Hephaestin and Ireg1 revealed in studies of genetic and nutritional iron deficiency., PMID:12730111

Cellular location of proteins related to iron absorption and transport., PMID:11891802

Iron absorption and transport., PMID:10522550

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-HEPH Polyclonal Antibody [PHJ49001]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only