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Anti-CD273/PD-L2/PDCD1LG2 Polyclonal Antibody

Catalog #:   PHJ48501 Specific References (19) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BQ51
Overview

Catalog No.

PHJ48501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD273/PD-L2/PDCD1LG2 (Leu20-Thr220).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PDCD1 ligand 2,B7-DC,PD-L2,PDCD1LG2,PDCD1L2,PDL2,Butyrophilin B7-DC,Programmed death ligand 2,Programmed cell death 1 ligand 2,CD273,B7DC,PD-1 ligand 2

Purification

Purified by antigen affinity column.

Accession

Q9BQ51

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CD273 / PD-L2 / PDCD1LG2 antibody (PHJ48501) at 1μg/ml.

    Lane 1: Raji cell lysate
    Lane 2: HepG2 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 32 kDa
  • Immunocytochemistry/ Immunofluorescence
    CD273 / PD-L2 / PDCD1LG2 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CD273 / PD-L2 / PDCD1LG2 (PHJ48501) at 20 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD273/PD-L2/PDCD1LG2 antibody (PHJ48501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood., PMID:36289665

Detrimental Effect of Trypanosoma brucei brucei Infection on Memory B Cells and Host Ability to Recall Protective B-cell Responses., PMID:35363871

Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells., PMID:33631789

Peptide Blocking CTLA-4 and B7-1 Interaction., PMID:33419027

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions., PMID:32324337

The Controversial Role of PD-1 and Its Ligands in Gynecological Malignancies., PMID:31681606

A role for B cells in organic dust induced lung inflammation., PMID:29273051

CD73 expression identifies a subset of IgM+ antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent., PMID:28746783

PD-1 function in apoptosis of T lymphocytes in canine visceral leishmaniasis., PMID:27016050

Cutting Edge: CTLA-4Ig Inhibits Memory B Cell Responses and Promotes Allograft Survival in Sensitized Recipients., PMID:26416270

Antagonist antibodies to PD-1 and B7-H1 (PD-L1) in the treatment of advanced human cancer., PMID:23460533

Memory B cells in the lung participate in protective humoral immune responses to pulmonary influenza virus reinfection., PMID:22308386

Aging-associated B7-DC+ B cells enhance anti-tumor immunity via Th1 and Th17 induction., PMID:22044484

Cutting edge: Hierarchy of maturity of murine memory B cell subsets., PMID:21078902

New monoclonal antibodies against B-cell antigens: possible new strategies for diagnosis of primary cutaneous B-cell lymphomas., PMID:20951741

Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line., PMID:20628373

B7-H1 on myeloid-derived suppressor cells in immune suppression by a mouse model of ovarian cancer., PMID:18790673

"Alternatively activated" dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig., PMID:17056511

Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation., PMID:15982311

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CD273/PD-L2/PDCD1LG2 Polyclonal Antibody [PHJ48501]
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