Catalog No.
PHJ46101
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SMAD5 (Gly210-Val464).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
SMAD 5,Mothers against decapentaplegic homolog 5,SMAD5,Mothers against DPP homolog 5,Smad5,SMAD family member 5,MAD homolog 5,JV5-1,MADH5,hSmad5
Purification
Purified by antigen affinity column.
Accession
Q99717
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling., PMID:39196179
EpicTope: narrating protein sequence features to identify non-disruptive epitope tagging sites., PMID:38559275
Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF-κB Signaling Pathway following Stimulation of the Inflammatory Microenvironment., PMID:35530413
Predictive model based on gene and laboratory data for intravenous immunoglobulin resistance in Kawasaki disease in a Chinese population., PMID:34174887
Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival., PMID:34013359
Up-regulation of miR-155 potentiates CD34+ CML stem/progenitor cells to escape from the growth-inhibitory effects of TGF-ß1 and BMP signaling., PMID:33907541
Impact of vaccine type on HIV-1 vaccine elicited antibody durability and B cell gene signature., PMID:32747654
Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections., PMID:30414134
Erythroferrone inhibits the induction of hepcidin by BMP6., PMID:30097509
Autoantibodies targeting TLR and SMAD pathways define new subgroups in systemic lupus erythematosus., PMID:29576246
Activin A amplifies dysregulated BMP signaling and induces chondro-osseous differentiation of primary connective tissue progenitor cells in patients with fibrodysplasia ossificans progressiva (FOP)., PMID:29170109
Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6., PMID:28815688
Transforming growth factor-β stimulates Smad1/5 signaling in pulmonary artery smooth muscle cells and fibroblasts of the newborn mouse through ALK1., PMID:28642261
BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5., PMID:28489883
Activation of CD137 Signaling Promotes Angiogenesis in Atherosclerosis via Modulating Endothelial Smad1/5-NFATc1 Pathway., PMID:28288971
Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma., PMID:28199984
The SMAD Pathway Is Required for Hepcidin Response During Endoplasmic Reticulum Stress., PMID:27483343
Partial Müllerian Duct Retention in Smad4 Conditional Mutant Male Mice., PMID:27194944
Bone Morphogenic Protein 4-Smad-Induced Upregulation of Platelet-Derived Growth Factor AA Impairs Endothelial Function., PMID:26769046
Therapeutic potential of regulatory cytokines that target B cells., PMID:26647406
Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling., PMID:26071935
Hyaluronan inhibits BMP-induced osteoblast differentiation., PMID:25592835
Targeting eIF4GI translation initiation factor affords an attractive therapeutic strategy in multiple myeloma., PMID:24815186
The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain., PMID:24495875
MicroRNA-155 controls RB phosphorylation in normal and malignant B lymphocytes via the noncanonical TGF-β1/SMAD5 signaling module., PMID:24136167
SnoN facilitates ALK1-Smad1/5 signaling during embryonic angiogenesis., PMID:24019535
Spatio-temporal distribution of Smads and role of Smads/TGF-β/BMP-4 in the regulation of mouse bladder organogenesis., PMID:23620745
TGF-β1, Smad-2/-3, Smad-1/-5/-8, and Smad-4 signaling factors are expressed in ameloblastomas, adenomatoid odontogenic tumors, and calcifying cystic odontogenic tumors: an immunohistochemical study., PMID:23157422
Sensing BMP pathway activity by immune detection of phosphorylated R-Smad proteins in mouse embryonic kidney., PMID:22639269
Chondroitin sulfate-E fine-tunes osteoblast differentiation via ERK1/2, Smad3 and Smad1/5/8 signaling by binding to N-cadherin and cadherin-11., PMID:22440395
Smad1/Smad5 signaling in limb ectoderm functions redundantly and is required for interdigital programmed cell death., PMID:22240098
Smad 1/5 is involved in bone morphogenetic protein-2-induced odontoblastic differentiation in human dental pulp cells., PMID:22152623
Hic-5 controls BMP4 responses in prostate cancer cells through interacting with Smads 1, 5 and 8., PMID:21996749
Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice., PMID:21810858
Loss of Smad5 leads to the disassembly of the apical junctional complex and increased susceptibility to experimental colitis., PMID:21212325
CRIM1 is expressed at higher levels in drug-resistant than in drug-sensitive myeloid leukemia HL60 cells., PMID:21036735
Galectin-9 induces osteoblast differentiation through the CD44/Smad signaling pathway., PMID:20206131
Human adipose-derived stromal cells respond to and elaborate bone morphogenetic protein-2 during in vitro osteogenic differentiation., PMID:20124834
Evidence for the prevention of enthesitis in HLA-B27/hβ(2)m transgenic rats treated with a monoclonal antibody against TNF-α., PMID:20015205
Characterization of mammary epithelial cell line HC11 using the NIA 15k gene array reveals potential regulators of the undifferentiated and differentiated phenotypes., PMID:19523745
Real time monitoring of BMP Smads transcriptional activity during mouse development., PMID:18615729
Mechanisms of murine lacrimal gland repair after experimentally induced inflammation., PMID:18586880
Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad., PMID:18310319
Detection, identification and in vivo treatment responsiveness of bone morphogenetic protein (BMP)-activated cell populations in the synovium of patients with rheumatoid arthritis., PMID:18276742
Development of a rabbit monoclonal antibody group against Smads and immunocytochemical study of human and mouse embryonic stem cells., PMID:18158783
SMAD expression in the testis: an insight into BMP regulation of spermatogenesis., PMID:18069690
Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells., PMID:17962458
Bone morphogenetic protein signaling pathway plays multiple roles during gastrointestinal tract development., PMID:16110505
BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1., PMID:15877825
Target validation for genomics using peptide-specific phage antibodies: a study of five gene products overexpressed in colorectal cancer., PMID:12209988
For research use only. Not for human or drug use.
