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Anti-NEIL1 Polyclonal Antibody

Catalog #:   PHJ39201 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q96FI4
Overview

Catalog No.

PHJ39201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NEIL1 (Ser43-Ala314).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Endonuclease 8-like 1,DNA glycosylase/AP lyase Neil1,Endonuclease VIII-like 1,Nei-like protein 1,DNA-(apurinic or apyrimidinic site) lyase Neil1,Nei homolog 1,NEIL1,FPG1,NEH1

Purification

Purified by antigen affinity column.

Accession

Q96FI4

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NEIL1 antibody (PHJ39201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Immunocytochemistry/ Immunofluorescence
    NEIL1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with NEIL1 (PHJ39201) at 17.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Development and characterization of a novel NEIL1 nanobody., PMID:40440929

Deciphering the crystal structure of a novel nanobody against the NEIL1 DNA glycosylase., PMID:38289715

Deciphering the orthorhombic crystal structure of a novel NEIL1 nanobody with pseudo-merohedral twinning., PMID:37609231

Autoantibody screening of plasma and peritoneal fluid of patients with endometriosis., PMID:36749097

DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model., PMID:34186496

NEIL1 is a candidate gene associated with common variable immunodeficiency in a patient with a chromosome 15q24 deletion., PMID:28093361

Base and Nucleotide Excision Repair of Oxidatively Generated Guanine Lesions in DNA., PMID:26733197

An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells., PMID:23405159

Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansion., PMID:19782007

Human NEIL1 localizes with the centrosomes and condensed chromosomes during mitosis., PMID:17556049

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-NEIL1 Polyclonal Antibody [PHJ39201]
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