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Anti-Human RHOA Polyclonal Antibody

Catalog #:   PHF45101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P61586
Overview

Catalog No.

PHF45101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RHOA (Ala2-Gly189).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Rho cDNA clone 12, ARH12, RHO12, ARHA, RHOA, Transforming protein RhoA, h12

Purification

Purified by antigen affinity column.

Accession

P61586

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RHOA antibody (PHF45101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 26 kDa
References

Comparison of the mutational profile between BCL2- and BCL6-rearrangement positive follicular lymphoma., PMID:40482882

Epithelial eversion, a collective rearrangement from apical-in to apical-out polarity, is initiated by α6β4 integrins and sustained by increased cell proliferation and anchorage-independence., PMID:40463241

Biological and Clinical Relevance of Genetic Alterations in Peripheral T-cell Lymphomas., PMID:40416001

PRRG4 Brain-Specific Conditional Knockout Mice Display Autism Spectrum Disorder-Like Behaviors., PMID:40380139

Orphan GPR50 Restrains Neurite Outgrowth and Cell Migration by Activating the G12/13 Protein-RhoA Pathway in Neural Progenitor Cells and Tanycytes., PMID:40091563

Binding of RHOA G17V to p300 enhances its HAT activity: a new mechanism of epigenetic deregulation in TFH lymphoma., PMID:40020168

The potential role of RhoA/ROCK-inhibition on locomotor recovery after spinal cord injury: a systematic review of in-vivo studies., PMID:39956860

In Vitro Experiment Present ROCK2 Inhibition Promotes the Therapeutic Effect of Bevacizumab in the Treatment of Glioblastoma Multiforme., PMID:39792535

Tetraspanin CD81 serves as a functional entry factor for porcine circovirus type 2 infection., PMID:39745447

Anillin interacts with RhoA to promote tumor progression in anaplastic thyroid cancer by activating the PI3K/AKT pathway., PMID:39738892

Regulation of BCR-dependent germinal center B-cell formation by HGAL and insight into its emerging myeloid ortholog, C1ORF150., PMID:39474423

Focal Adhesion Kinase (FAK) inhibition induces membrane accumulation of aquaporin2 (AQP2) through endocytosis inhibition and actin depolymerization in renal epithelial cells., PMID:39416213

IL-8 promotes lens capsular residual cells migration by down-regulates expression of E-cadherin and ZO-1 via the CXCR1/2-NF-κB-RhoA signal pathway., PMID:39244903

CD64+ fibroblast-targeted vilanterol and a STING agonist augment CLDN18.2 BiTEs efficacy against pancreatic cancer by reducing desmoplasia and enriching stem-like CD8+ T cells., PMID:39187291

IL-13 and IL-17A activate β1 integrin through an NF-kB/Rho kinase/PIP5K1γ pathway to enhance force transmission in airway smooth muscle., PMID:39136993

A combination of 5-azacytidine and nivolumab is a potentially effective rescue therapy in relapsed/refractory AITL., PMID:38983865

Tumor necrosis factor α receptor 1A transduces the inhibitory effect on axon regeneration triggered by IgG anti-ganglioside GD1a antibodies., PMID:38897255

Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by the Inhibition of Active RhoA at Cell-Cell Adhesions., PMID:38766211

IL-13 and IL-17A Activate β1 Integrin through an NF-kB/Rho kinase/PIP5K1γ pathway to Enhance Force Transmission in Airway Smooth Muscle., PMID:38746410

The ion channel TRPV5 regulates B-cell signaling and activation., PMID:38694510

Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement., PMID:38462571

Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling., PMID:38397010

PLK1 phosphorylates RhoGDI1 and promotes cancer cell migration and invasion., PMID:38355643

Geranylgeranyl isoprenoids and hepatic Rap1a regulate basal and statin-induced expression of PCSK9., PMID:38309417

Suppression of Mast Cell Activation by GPR35: GPR35 Is a Primary Target of Disodium Cromoglycate., PMID:38290974

Angiogenic and Fibrogenic Dual-effect of Gremlin1 on Proliferative Diabetic Retinopathy., PMID:38250154

Fluorescent peptide for detecting factor XIIIa activity and fibrin in whole blood clots forming under flow., PMID:38222077

Placenta microRNA profile of patient with Obstetric Antiphospholipid Syndrome., PMID:38155384

Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function., PMID:38049580

Blockade of Rho-associated kinase prevents inhibition of axon regeneration of peripheral nerves induced by anti-ganglioside antibodies., PMID:37843226

RhoA vesicle trafficking-mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy., PMID:37811653

Extracellular pyruvate kinase M2 induces cell migration through p-Tyr42 RhoA-mediated superoxide generation and epithelial-mesenchymal transition., PMID:37722568

RhoA-LIMK Signaling Axis Reveals Rostral-Caudal Plane and Spatial Dysregulation in the Brain of Alzheimer's Disease Mouse Models., PMID:37718806

Composite B-cell and T-cell lymphomas: clinical, pathological, and molecular features of three cases and literature review., PMID:37551557

IGF2BP3/HIF1A/YAP signaling plays a role in driving acute-on-chronic liver failure through activating hepatocyte reprogramming., PMID:37257765

Antibodies Directed Against GalNAc- and GlcNAc-O-Tyrosine Posttranslational Modifications - a New Tool for Glycoproteomic Detection., PMID:37052513

Vascular mechanisms of post-COVID-19 conditions: Rho-kinase is a novel target for therapy., PMID:37019821

Pathologic and molecular insights in nodal T-follicular helper cell lymphomas., PMID:36793612

Endothelial-Specific Targeting of RhoA Signaling via CD31 Antibody-Conjugated Nanoparticles., PMID:36746610

ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells., PMID:36631535

Flow Cytometry Analysis Reveals a Wide Cytologic and Immunophenotypic Spectrum of Peripheral B Lymphocytes in Angioimmunoblastic T-Cell Lymphoma., PMID:36423589

Involvement of Paired Immunoglobulin-Like Receptor B in Cognitive Dysfunction Through Hippocampal-Dependent Synaptic Plasticity Impairments in Mice Subjected to Chronic Sleep Restriction., PMID:36417104

CD16+ fibroblasts foster a trastuzumab-refractory microenvironment that is reversed by VAV2 inhibition., PMID:36379207

Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes., PMID:36111670

Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling., PMID:36059552

Clonal germinal center B cells function as a niche for T-cell lymphoma., PMID:35921527

Stool Interleukin-1β Differentiates Clostridioides difficile Infection (CDI) From Asymptomatic Carriage and Non-CDI Diarrhea., PMID:35906836

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells., PMID:35883755

Autocrine pro-legumain promotes breast cancer metastasis via binding to integrin αvβ3., PMID:35854065

The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury., PMID:35784350

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human RHOA Polyclonal Antibody [PHF45101]
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