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Anti-Human POLQ Polyclonal Antibody

Catalog #:   PHB19401 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O75417
Overview

Catalog No.

PHB19401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POLQ (Met1-Met894).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

POLH, DNA polymerase eta, DNA polymerase theta, POLQ

Purification

Purified by antigen affinity column.

Accession

O75417

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with POLQ antibody (PHB19401) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 112 kDa
    Observed MW: 112 kDa
References

Analysis of translesion polymerases in colorectal cancer cells following cetuximab treatment: A network perspective., PMID:39102671

Mechanism of suppression of chromosomal instability by DNA polymerase POLQ., PMID:25275444

DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken., PMID:20584917

Absence of DNA polymerase theta results in decreased somatic hypermutation frequency and altered mutation patterns in Ig genes., PMID:16890500

DNA polymerases and somatic hypermutation of immunoglobulin genes., PMID:16319960

DNA polymerase theta contributes to the generation of C/G mutations during somatic hypermutation of Ig genes., PMID:16172387

POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in human cells., PMID:14576298

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human POLQ Polyclonal Antibody [PHB19401]
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