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Anti-RAD50 Polyclonal Antibody

Catalog #:   PHJ34201 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92878
Overview

Catalog No.

PHJ34201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAD50 (Thr437-Val792).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RAD50,hRAD50,DNA repair protein RAD50

Concentration

2.02 mg/ml

Purification

Purified by antigen affinity column.

Accession

Q92878

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with RAD50 antibody (PHJ34201) at 1ug/mL .

    Lane 1: K562 cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: MOLT-4 cell lysate
    Lane 4: PC-12 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 155 kDa
    Observed MW: 155 kDa
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RAD50 antibody (PHJ34201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 44 kDa
    Observed MW: 44 kDa
References

SingleNucleotide Polymorphisms as Biomarkers of Mepolizumab and Benralizumab Treatment Response in Severe Eosinophilic Asthma., PMID:39125709

Genomic analysis of plasma circulating tumor DNA in patients with heavily pretreated HER2 + metastatic breast cancer., PMID:37336919

Topological Analysis of γH2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair., PMID:34771723

MRE11 promotes oral cancer progression through RUNX2/CXCR4/AKT/FOXA2 signaling in a nuclease-independent manner., PMID:33927349

Precision medicine phase II study evaluating the efficacy of a double immunotherapy by durvalumab and tremelimumab combined with olaparib in patients with solid cancers and carriers of homologous recombination repair genes mutation in response or stable after olaparib treatment., PMID:32778095

Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers., PMID:31473966

Localization Microscopy Analyses of MRE11 Clusters in 3D-Conserved Cell Nuclei of Different Cell Lines., PMID:29361783

EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork-Associated DNA Damage Repair., PMID:28801308

Panel of SEREX-defined antigens for breast cancer autoantibodies profile detection., PMID:27775439

DNA damage precedes apoptosis during the regression of the interdigital tissue in vertebrate embryos., PMID:27752097

Polymorphisms of RAD50, IL33 and IL1RL1 are associated with atopic asthma in Chinese population., PMID:26493291

RAD50 Single-Nucleotide Polymorphism in Predominantly Antibody Deficiency., PMID:26310047

Targeting of β1 integrins impairs DNA repair for radiosensitization of head and neck cancer cells., PMID:26073085

Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures., PMID:26004008

Identification of Plasmodium falciparum DNA Repair Protein Mre11 with an Evolutionarily Conserved Nuclease Function., PMID:25938776

Microtubule-targeting agents augment the toxicity of DNA-damaging agents by disrupting intracellular trafficking of DNA repair proteins., PMID:25605897

Identification of the interactors of human nibrin (NBN) and of its 26 kDa and 70 kDa fragments arising from the NBN 657del5 founder mutation., PMID:25485873

The human SRCAP chromatin remodeling complex promotes DNA-end resection., PMID:25176633

Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells., PMID:25084169

Smad7 enhances ATM activity by facilitating the interaction between ATM and Mre11-Rad50-Nbs1 complex in DNA double-strand break repair., PMID:25063542

Fine-mapping of IgE-associated loci 1q23, 5q31, and 12q13 using 1000 Genomes Project data., PMID:24930997

A polymorphism in the TH 2 locus control region is associated with changes in DNA methylation and gene expression., PMID:24866380

Sym004, a novel anti-EGFR antibody mixture, augments radiation response in human lung and head and neck cancers., PMID:24130052

Human candidate polymorphisms in sympatric ethnic groups differing in malaria susceptibility in Mali., PMID:24098393

Multicolor laser scanning confocal immunofluorescence microscopy of DNA damage response biomarkers., PMID:23896884

Ionizing-radiation induced DNA double-strand breaks: a direct and indirect lighting up., PMID:23849169

The MRN-CtIP pathway is required for metaphase chromosome alignment., PMID:23434370

SMC1-mediated intra-S-phase arrest facilitates bocavirus DNA replication., PMID:23365434

Immunohistochemical analysis of medullary breast carcinoma autoantigens in different histological types of breast carcinomas., PMID:23181716

Current concepts of IgE regulation and impact of genetic determinants., PMID:22909159

The Alström syndrome protein, ALMS1, interacts with α-actinin and components of the endosome recycling pathway., PMID:22693585

Activation of protein kinase Tel1 through recognition of protein-bound DNA ends., PMID:21402778

L1CAM regulates DNA damage checkpoint response of glioblastoma stem cells through NBS1., PMID:21297581

Autophosphorylation and ATM activation: additional sites add to the complexity., PMID:21149446

Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex., PMID:20943970

Unique DNA repair gene variations and potential associations with the primary antibody deficiency syndromes IgAD and CVID., PMID:20805886

Loss of juxtaposition of RAG-induced immunoglobulin DNA ends is implicated in the precursor B-cell differentiation defect in NBS patients., PMID:20378756

Temporal regulation of Ig gene diversification revealed by single-cell imaging., PMID:19748985

Multiple functions of MRN in end-joining pathways during isotype class switching., PMID:19633670

Meiotic localization of Mre11 and Rad50 in wild type, spo11-1, and MRN complex mutants of Coprinus cinereus., PMID:19396455

Genetic evidence for single-strand lesions initiating Nbs1-dependent homologous recombination in diversification of Ig v in chicken B lymphocytes., PMID:19180185

Genome-wide scan on total serum IgE levels identifies FCER1A as novel susceptibility locus., PMID:18846228

A tool for enhancement and scoring of DNA repair foci., PMID:18836995

Structure of a second BRCT domain identified in the nijmegen breakage syndrome protein Nbs1 and its function in an MDC1-dependent localization of Nbs1 to DNA damage sites., PMID:18582474

Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica., PMID:18402694

Mechanism and regulation of class switch recombination., PMID:18370922

The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius., PMID:18294364

ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain., PMID:17706468

Defective signal joint recombination in fanconi anemia fibroblasts reveals a role for Rad50 in V(D)J recombination., PMID:17524422

Pol eta is required for DNA replication during nucleotide deprivation by hydroxyurea., PMID:17369853

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-RAD50 Polyclonal Antibody [PHJ34201]
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