Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-CD112/Nectin-2/NECTIN2 Polyclonal Antibody

Catalog #:   PHJ32501 Specific References (39) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92692
Overview

Catalog No.

PHJ32501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD112/Nectin-2/NECTIN2 (Gln32-Pro350).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Herpesvirus entry mediator B,Herpes virus entry mediator B,Nectin cell adhesion molecule 2,HVEB,PVRL2,Poliovirus receptor-related protein 2,CD112,PRR2,Nectin-2,HveB,NECTIN2

Purification

Purified by antigen affinity column.

Accession

Q92692

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PVRL2 antibody (PHJ32501) at 0.3ug/ml.

    Lane 1: 293T cell lysate
    Lane 2: THP1 cell lysate
    Lane 3: HepG2 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 57 kDa
    Observed MW: 57,70 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD112/Nectin-2/NECTIN2 antibody (PHJ32501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

Co-blocking TIGIT and PVRIG Using a Novel Bispecific Antibody Enhances Antitumor Immunity., PMID:39851063

CD34+ and CD34- MM cells show different immune-checkpoint molecule expression profiles: high expression of CD112 and CD137 ligand on CD34+ MM cells., PMID:39531203

Clinical response and pathway-specific correlates following TIGIT-LAG3 blockade in myeloma: the MyCheckpoint randomized clinical trial., PMID:39187595

CD112 Supports Lymphatic Migration of Human Dermal Dendritic Cells., PMID:38474388

Combined Blockade of TIGIT and PD-L1 Enhances Anti-Neuroblastoma Efficacy of GD2-Directed Immunotherapy with Dinutuximab Beta., PMID:37444427

Single-cell RNA sequencing highlights the role of PVR/PVRL2 in the immunosuppressive tumour microenvironment in hepatocellular carcinoma., PMID:37383234

Emergence of the CD226 Axis in Cancer Immunotherapy., PMID:35812451

TgMab-2: An Anti-human T Cell Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Motif Domain Monoclonal Antibody for Immunocytochemistry., PMID:35736625

Gene of the month: T-cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT)., PMID:35058314

A reporter gene assay for determining the biological activity of therapeutic antibodies targeting TIGIT., PMID:35024316

CD155 and CD112 as possible therapeutic targets of FLT3 inhibitors for acute myeloid leukemia., PMID:34992684

A descriptive analysis of non-human leukocyte antigens present in renal transplant donor-recipient pairs., PMID:34582968

Innate Immune Checkpoint Inhibitors: The Next Breakthrough in Medical Oncology?, PMID:33850005

CD112 Regulates Angiogenesis and T Cell Entry into the Spleen., PMID:33467729

Expansion of circulating peripheral TIGIT+CD226+ CD4 T cells with enhanced effector functions in dermatomyositis., PMID:33413573

TIGIT: a novel immunotherapy target moving from bench to bedside., PMID:30232519

Immune checkpoints PVR and PVRL2 are prognostic markers in AML and their blockade represents a new therapeutic option., PMID:29855615

TX99 Is a Neutralizing Monoclonal Antibody Against Mouse TIGIT., PMID:29648914

Development and Characterization of Novel Monoclonal Antibodies Against Human DNAM-1., PMID:28498033

Nectin-2 (CD112) Is Expressed on Outgrowth Endothelial Cells and Regulates Cell Proliferation and Angiogenic Function., PMID:27676263

Anti-CD155 and anti-CD112 monoclonal antibodies conjugated to a fluorescent mesoporous silica nanosensor encapsulating rhodamine 6G and fluorescein for sensitive detection of liver cancer cells., PMID:27301350

CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells., PMID:27296670

Recombinant soluble CD226 protein directly inhibits cancer cell proliferation in vitro., PMID:24468679

CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain., PMID:24451371

Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration., PMID:24116228

Establishment and characterization of a novel anti-DNAM-1 monoclonal antibody., PMID:23600509

Crystal structure of cell adhesion molecule nectin-2/CD112 and its binding to immune receptor DNAM-1/CD226., PMID:22547693

Identification and functional analysis of ligands for natural killer cell activating receptors in colon carcinoma., PMID:22189020

The cell surface proteome of human mesenchymal stromal cells., PMID:21637820

Identification of endothelial cell junctional proteins and lymphocyte receptors involved in transendothelial migration of human effector memory CD4+ T cells., PMID:21191062

A novel interface consisting of homologous immunoglobulin superfamily members with multiple functions., PMID:20081873

[Preparation and characterization of monoclonal antibodies against human CD112 (Nectin2/PRR2)]., PMID:17428395

The switch from latent to productive infection in epstein-barr virus-infected B cells is associated with sensitization to NK cell killing., PMID:17079298

Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process., PMID:16831868

PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis., PMID:15607800

DNAM-1 and PVR regulate monocyte migration through endothelial junctions., PMID:15136589

Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112)., PMID:15039383

Identification of PVR (CD155) and Nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule., PMID:12913096

Retinoid regulation of human cathepsin D gene expression., PMID:8639464

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-CD112/Nectin-2/NECTIN2 Polyclonal Antibody [PHJ32501]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only