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Anti-Strep Tag (WSHPQFEK) Monoclonal Antibody (1A189)

Catalog #:   MGK26104 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG
Applications: ELISA, WB
Overview

Catalog No.

MGK26104

Species reactivity

General

Host species

Mouse

Isotype

IgG

Clonality

Monoclonal

Immunogen

Strep Tag (WSHPQFEK)

Tested applications

ELISA: 1:2000-1:8000, IHC: 1:50-1:500, WB: 1:1000-1:5000

Target

Strep Tag (WSHPQFEK), Strep Tag,streptag,StrepTag II

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

ELISA, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

1A189

Data Image
References

The extracellular region of Trypanosoma congolense Membrane Bound Acid Phosphatase induces partial protection in immunized BALB/c mice., PMID:40185169

Bunyaviruses: Expression of Recombinant Glycoproteins from Insect Cells., PMID:39671038

The N-terminal domain is required for cell surface localisation of VapA, a member of the Vap family of Rhodococcus equi virulence proteins., PMID:38421980

Recombinantly Expressed Tagged SUrface Protein (RETSUP) assay: a new diagnostic system for the detection of antibodies to platelets., PMID:38184205

Heterologous DNA-prime/protein-boost immunization with a monomeric SARS-CoV-2 spike antigen redundantizes the trimeric receptor-binding domain structure to induce neutralizing antibodies in old mice., PMID:37753087

Soluble SARS-CoV-2 RBD and human ACE2 peptidase domain produced in Drosophila S2 cells show functions evoking virus-cell interface., PMID:37405395

Immunomagnetic Separation Method Integrated with the Strep-Tag II System for Rapid Enrichment and Mild Release of Exosomes., PMID:36661256

Expression, purification, and kinetic characterization of the human strep-IDO1., PMID:35706807

TOOLBOX: Strep‑Tagged nano‑assemblies of antibody‑drug‑conjugates (ADC) for modular and conditional cancer drugging., PMID:34105341

Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination., PMID:30279478

In vitro-Formulated Oligomers of Strep-Tagged Avian Influenza Haemagglutinin Produced in Plants Cause Neutralizing Immune Responses., PMID:30177967

Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1., PMID:30049287

Development of a serodiagnostic IgM-ELISA for tick-borne encephalitis virus using subviral particles with strep-tag., PMID:29960872

Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain., PMID:29143175

Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles., PMID:26969490

Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy., PMID:26900664

A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS., PMID:26088135

Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa., PMID:25914160

Self-processing of a barley subtilase expressed in E. coli., PMID:24927642

Expression and purification of recombinant antibody formats and antibody fusion proteins., PMID:24515473

Sortase-catalyzed in vitro functionalization of a HER2-specific recombinant Fab for tumor targeting of the plant cytotoxin gelonin., PMID:24492291

Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans., PMID:23791917

Protective efficacy of Newcastle disease virus expressing soluble trimeric hemagglutinin against highly pathogenic H5N1 influenza in chickens and mice., PMID:22952980

Involvement of soluble Fas Ligand in germ cell apoptosis in testis of rats undergoing autoimmune orchitis., PMID:22892327

Novel serial positive enrichment technology enables clinical multiparameter cell sorting., PMID:22545138

A combined strategy improves the solubility of aggregation-prone single-chain variable fragment antibodies., PMID:22387083

Analysis of interaction partners for eukaryotic translation elongation factor 1A M-domain by functional proteomics., PMID:21689717

Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange., PMID:20198304

Unexpected oligomeric structure of the FocA formate channel of Escherichia coli : a paradigm for the formate-nitrite transporter family of integral membrane proteins., PMID:20041954

A biotin-hydrogel-coated quartz crystal microbalance biosensor and applications in immunoassay and peptide-displaying cell detection., PMID:19464992

Multistep processing of an insertion sequence in an essential subunit of the chloroplast ClpP complex., PMID:19346247

Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae., PMID:19243080

Tagging of MADS domain proteins for chromatin immunoprecipitation., PMID:17868439

Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life., PMID:17595342

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins., PMID:17571060

Methods for preparation of low abundance glycoproteins from mammalian cell supernatants., PMID:16822540

Gramicidin-based channel systems for the detection of protein-ligand interaction., PMID:15018906

Non-natural cell surface receptors: synthetic peptides capped with N-cholesterylglycine efficiently deliver proteins into Mammalian cells., PMID:12526694

[Rapid immunotitration of individual toxins from Androctonus australis venom]., PMID:12404870

Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen., PMID:12089001

Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes., PMID:11936096

Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold., PMID:11688717

In vitro selection of other proteins than antibodies by means of ribosome display., PMID:11478944

Use of thiophilic adsorption chromatography for the one-step purification of a bacterially produced antibody F(ab) fragment without the need for an affinity tag., PMID:10600461

Recombinant human tissue transglutaminase ELISA for the diagnosis of gluten-sensitive enteropathy., PMID:10585346

Construction and characterization of a quadruplex DNA selective single-chain autoantibody from a viable motheaten mouse hybridoma with homology to telomeric DNA binding proteins., PMID:9819226

Construction and expression of a bifunctional single-chain antibody against Bacillus cereus p6ores., PMID:9647820

Multiple affinity domains for the detection, purification and immobilization of recombinant proteins., PMID:9174944

Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunocytochemistry., PMID:8648079

Use of antibody fragments (Fv) in immunocytochemistry., PMID:7769231

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Strep Tag (WSHPQFEK) Monoclonal Antibody (1A189) [MGK26104]
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