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Anti-CD177 Polyclonal Antibody

Catalog #:   PHJ22801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q8N6Q3
Overview

Catalog No.

PHJ22801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD177 (Cys133-Ser301).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

CD177,NB1 GP,HNA-2a,Human neutrophil alloantigen 2a,NB1,PRV-1,CD177 antigen,Polycythemia rubra vera protein 1,NB1 glycoprotein,PRV1

Purification

Purified by antigen affinity column.

Accession

Q8N6Q3

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD177 antibody (PHJ22801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with CD177 antibody (PHJ22801) at 1 μg/ml.

    Lane 1: Mouse brain
    Lane 2: Human placenta

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 46 kDa
    Observed MW: 46 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human bone marrow tissue stained for CD177 with PHJ22801.
References

Glycosylation-driven programs coordinate immunoregulatory and pro-angiogenic functions of myeloid-derived suppressor cells., PMID:40381622

The tumor cell killing capacity of head and neck cancer patient-derived neutrophils depends on tumor stage, gender and the antibody isotype., PMID:39306937

CD177 is a novel IgG Fc receptor and CD177 genetic variants affect IgG-mediated function., PMID:39131159

The CD177 c.1291A Allele Leads to a Loss of Membrane Expression and Mimics a CD177-Null Phenotype., PMID:38474126

CD177 is a novel IgG Fc receptor and CD177 genetic variants affect IgG-mediated function., PMID:38260289

Disease Activity and Tendency to Relapse in ANCA-Associated Vasculitis Are Reflected in Neutrophil and Intermediate Monocyte Frequencies., PMID:38213873

OMIP-100: A flow cytometry panel to investigate human neutrophil subsets., PMID:38179854

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783

Immune Profiling Panel Gene Set Identifies Critically Ill Patients With Low Monocyte Human Leukocyte Antigen-DR Expression: Preliminary Results From the REAnimation Low Immune Status Marker (REALISM) Study., PMID:36917594

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians., PMID:36818775

Protective α1-antitrypsin effects in autoimmune vasculitis are compromised by methionine oxidation., PMID:36125911

Competitively disrupting the neutrophil-specific receptor-autoantigen CD177:proteinase 3 membrane complex reduces anti-PR3 antibody-induced neutrophil activation., PMID:35063507

Human neutrophil antigen 2 sequence-based typing: Joining the hunt for the CD177 answer., PMID:34590317

Transfusion of target antigens to preimmunized recipients: a new mechanism in transfusion-related acute lung injury., PMID:34438443

Chronic E-Cigarette Aerosol Inhalation Alters the Immune State of the Lungs and Increases ACE2 Expression, Raising Concern for Altered Response and Susceptibility to SARS-CoV-2., PMID:34122126

Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils., PMID:33767714

Characterization of CD177-reactive iso- and auto-antibodies., PMID:33734454

Distinct Gene Expression Signatures Characterize Strong Clinical Responders Versus Nonresponders to Canakinumab in Children With Systemic Juvenile Idiopathic Arthritis., PMID:33452871

Multicenter Study on Differential Human Neutrophil Antigen 2 Expression and Underlying Molecular Mechanisms., PMID:33173457

Human neutrophil antigens: Nature, clinical significance and detection., PMID:32970372

Dynamics of Cardiac Neutrophil Diversity in Murine Myocardial Infarction., PMID:32811295

Increase expression of CD177 in Kawasaki disease., PMID:30943984

Novel findings in neutrophil biology and their impact on cardiovascular disease., PMID:30918936

Determination of Subset-Restricted Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry., PMID:30539568

Molecular Genetics of the Human Neutrophil Antigens., PMID:30498408

Tumour-infiltrating neutrophils counteract anti-VEGF therapy in metastatic colorectal cancer., PMID:30377339

Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA., PMID:30236159

The Role of Neonatal Gr-1+ Myeloid Cells in a Murine Model of Rhesus-Rotavirus-Induced Biliary Atresia., PMID:30201498

CD117 Expression in Squamous Cell Carcinoma of the Oesophagus., PMID:29970514

Flow-Cytometric Analysis of HNA-2 Expression and Phenotypes Among Thai Blood Donors., PMID:29611387

Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay., PMID:28833244

[Gastrointestinal stromal tumor and renal transplant]., PMID:28825581

CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation., PMID:28807980

Characterization of the CD177 interaction with the ANCA antigen proteinase 3., PMID:28240246

IL-17RB+ granulocytes are associated with airflow obstruction in asthma., PMID:27979026

Prolonged neutropenia due to antihuman neutrophil antigen 2 (CD177) antibody after bone marrow transplantation., PMID:27905683

A sequence-specific polymerase chain reaction method for HNA-2 genotyping: homozygous c.843A>T mutation predicts the absence of CD177., PMID:27385476

Lessons from a double-transgenic neutrophil approach to induce antiproteinase 3 antibody-mediated vasculitis in mice., PMID:27365530

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion., PMID:27227454

Rapid characterization of hybridomas producing monoclonal antibodies against platelet β3 integrin using ELIspot., PMID:27185103

Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies., PMID:26149790

HNA antibody-mediated neutrophil aggregation is dependent on serine protease activity., PMID:26084778

Geno- and phenotyping of human neutrophil antigens., PMID:26024636

Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis., PMID:27102815

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177., PMID:24926686

Neutropenia associated with antihuman neutrophil antibodies following allogeneic hematopoietic stem cell transplantation., PMID:24237009

External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012., PMID:23663230

Gene expression profiling of whole blood in ipilimumab-treated patients for identification of potential biomarkers of immune-related gastrointestinal adverse events., PMID:23521917

[Biomarkers associated with unresponsiveness to IVIG in children with Kawasaki disease]., PMID:23445729

Identifying patients with neutrophil elastase (ELANE) mutations from patients with a presumptive diagnosis of autoimmune neutropenia., PMID:23206890

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CD177 Polyclonal Antibody [PHJ22801]
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