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Anti-TUBB3 Polyclonal Antibody

Catalog #:   PHG56701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q13509
Overview

Catalog No.

PHG56701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TUBB3 (Met1-Lys450).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain

Concentration

1.02 mg/ml

Purification

Purified by antigen affinity column.

Accession

Q13509

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with TUBB3 antibody (PHG56701) at 1ug/mL .

    Lane 1: N2A cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse testis lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat testis lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 55 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TUBB3 antibody (PHG56701) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 53 kDa
    Observed MW: 53 kDa
References

High Intraocular Concentration of Fibrinogen Regulates Retinal Function Via the ICAM-1 Pathway., PMID:39546292

Single-extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron-derived EVs., PMID:38868956

Regenerated Corneal Epithelium Expresses More βIII-Tubulin After Chemical Injuries Compared to Mechanical Injuries., PMID:38085248

HSP27 induced glaucomatous damage in mice of young and advanced age., PMID:37744880

Nogo-A Regulates the Fate of Human Dental Pulp Stem Cells toward Osteogenic, Adipogenic, and Neurogenic Differentiation., PMID:36359811

Randomised phase II trial of weekly ixabepilone ± biweekly bevacizumab for platinum-resistant or refractory ovarian/fallopian tube/primary peritoneal cancer., PMID:35149854

Nuclear IMPDH Filaments in Human Gliomas., PMID:34498062

Targeted mass spectrometry for monitoring of neural differentiation., PMID:34357391

Quantitative Multiplexed Proteomics Could Assist Therapeutic Decision Making in Non-Small Cell Lung Cancer Patients with Ambiguous ALK Test Results., PMID:34066104

Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

BET inhibition increases βIII-tubulin expression and sensitizes metastatic breast cancer in the brain to vinorelbine., PMID:32848091

Characterization of T-DM1-resistant breast cancer cells., PMID:32583565

Alpha- and beta-tubulin isotypes are differentially expressed during brain development., PMID:32293117

Monoclonal antibody AC10364 inhibits cell proliferation in 5-fluorouracil resistant hepatocellular carcinoma via apoptotic pathways., PMID:31303763

An in vitro developmental neurotoxicity screening assay for retinoic acid-induced neuronal differentiation using the human NT2/D1 cell line., PMID:30980846

iTRAQ-based pharmacoproteomics reveals potential targets of berberine, a promising therapy for ulcerative colitis., PMID:30771347

Optic Nerve Head Astrocytes Display Axon-Dependent and -Independent Reactivity in Response to Acutely Elevated Intraocular Pressure., PMID:30665231

Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis., PMID:29528446

Preoperative Anti-Class III β-Tubulin Antibodies As Relevant Clinical Biomarkers in Ovarian Cancer., PMID:29448203

High protein and mRNA expression levels of TUBB3 (class III ß-tubulin) are associated with aggressive tumor features in esophageal adenocarcinomas., PMID:29383151

Post-Translational Tubulin Modifications in Human Astrocyte Cultures., PMID:28512712

Comparison of the Effects of Curcumin and RG108 on NGF-Induced PC-12 Adh Cell Differentiation and Neurite Outgrowth., PMID:28338387

Partial Reprogramming of Pluripotent Stem Cell-Derived Cardiomyocytes into Neurons., PMID:28327614

Structure of neuro-endocrine and neuro-epithelial interactions in human foetal pancreas., PMID:27823763

Malignant glioma with primitive neuroectodermal tumor-like component (MG-PNET): novel microarray findings in a pediatric patient., PMID:27781423

Corneal Reinnervation and Sensation Recovery in Patients With Herpes Zoster Ophthalmicus: An In Vivo and Ex Vivo Study of Corneal Nerves., PMID:26989956

Induction of neural differentiation in rat C6 glioma cells with taxol., PMID:26665000

Human organotypic retinal cultures (HORCs) as a chronic experimental model for investigation of retinal ganglion cell degeneration., PMID:26432917

Coordinated interaction of Down syndrome cell adhesion molecule and deleted in colorectal cancer with dynamic TUBB3 mediates Netrin-1-induced axon branching., PMID:25754961

An agarose gel-based neurosphere culture system leads to enrichment of neuronal lineage cells in vitro., PMID:25539864

Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts., PMID:25343338

Tubulin β-III: a novel immunohistochemical marker for intrahepatic peripheral cholangiocarcinoma., PMID:25039376

Effect of all-trans retinoic acid on the differentiation of U87 glioma stem/progenitor cells., PMID:23852377

Autoantibodies against neuronal progenitors in sera from children with autism., PMID:23838310

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation., PMID:23430457

Blockade of VEGFR-1 and VEGFR-2 enhances paclitaxel sensitivity in gastric cancer cells., PMID:23364970

[Immunohistochemical characteristics of human brain vascular plexuses]., PMID:23342655

Use of high content image analyses to detect chemical-mediated effects on neurite sub-populations in primary rat cortical neurons., PMID:23142577

An angiogenesis model for investigating multicellular interactions across intact microvascular networks., PMID:23125212

Overexpression of class III beta tubulin and amplified HER2 gene predict good response to paclitaxel and trastuzumab therapy., PMID:23028798

In vitro differentiation of adult adipose mesenchymal stem cells into retinal progenitor cells., PMID:22907142

Myelin sheath decompaction, axon swelling, and functional loss during chronic secondary degeneration in rat optic nerve., PMID:22879411

[Molecular cloning of tubulin beta 3 (TUBB3) in Gekko japonicus and preparation of its polyclonal antibody]., PMID:22855447

Limiting multiple sclerosis related axonopathy by blocking Nogo receptor and CRMP-2 phosphorylation., PMID:22544872

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia., PMID:22134676

Identification of class III β-tubulin as a marker of angiogenic perivascular cells., PMID:21958528

Disease-free survival of patients after surgical resection of non-small cell lung carcinoma and correlation with excision repair cross-complementation group 1 expression and genotype., PMID:21899657

Neural stem/progenitor cells circulating in peripheral blood of patients with neovascular form of AMD: a novel view on pathophysiology., PMID:21847578

Optineurin inclusions occur in a minority of TDP-43 positive ALS and FTLD-TDP cases and are rarely observed in other neurodegenerative disorders., PMID:21360076

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-TUBB3 Polyclonal Antibody [PHG56701]
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