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Anti-Human SPACA1 Polyclonal Antibody

Catalog #:   PHN33401 Specific References (14) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9HBV2
Overview

Catalog No.

PHN33401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SPACA1 (Glu78-Thr201).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Sperm acrosomal membrane-associated protein 32, SAMP32, Sperm acrosome membrane-associated protein 1, SPACA1

Purification

Purified by antigen affinity column.

Accession

Q9HBV2

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SPACA1 antibody (PHN33401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
References

Bioinformatic prediction of the structure and characteristics of human sperm acrosome membrane-associated protein 1 (hSAMP32) and evaluation of its antifertility function in vivo., PMID:33220718

Cleavage of SPACA1 regulates assembly of sperm-egg membrane fusion machinery in mature spermatozoa†., PMID:31836887

Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects., PMID:31424156

Changes in the distribution and molecular mass of boar sperm acrosome-associated 1 proteins during the acrosome reaction; their validity as indicators for occurrence of the true acrosome reaction., PMID:27449406

Individual differences in the distribution of sperm acrosome-associated 1 proteins among male patients of infertile couples; their possible impact on outcomes of conventional in vitro fertilization., PMID:27185107

A Novel Testis-Specific Gene, Ccdc136, Is Required for Acrosome Formation and Fertilization in Mice., PMID:27076447

Acrosome markers of human sperm., PMID:26748928

Discovery of human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes and their effects on fertility in male and female mice., PMID:25209425

Case report of apoptosis in testis of four AZFc-deleted patients: increased DNA fragmentation during meiosis, but decreased apoptotic markers in post-meiotic germ cells., PMID:22508792

The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins., PMID:18448843

Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis., PMID:17643494

Identification of sperm immunoreactive antigens for immunocontraceptive purposes: a review., PMID:15035665

SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa., PMID:12606493

SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein., PMID:11870081

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human SPACA1 Polyclonal Antibody [PHN33401]
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