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Anti-Human STX4 Polyclonal Antibody

Catalog #:   PHG30801 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q12846
Overview

Catalog No.

PHG30801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STX4 (Ser36-Leu275).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Syntaxin-4, STX4A, Renal carcinoma antigen NY-REN-31, STX4

Purification

Purified by antigen affinity column.

Accession

Q12846

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with STX4 antibody (PHG30801) at 1 µg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HL-60 cell lysate
    Lane 3: K562 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 34 kDa
    Observed MW: 35 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with STX4 antibody (PHG30801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Tomosyn functions as a PKCδ-regulated fusion clamp in mast cell degranulation., PMID:29970602

Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation., PMID:27821849

Different activation signals induce distinct mast cell degranulation strategies., PMID:27643442

VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins., PMID:27616736

Syntaxin-4 is implicated in the secretion of antibodies by human plasma cells., PMID:24146186

SNAP-23 and syntaxin-3 are required for chemokine release by mature human mast cells., PMID:21981832

Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells., PMID:18253931

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human STX4 Polyclonal Antibody [PHG30801]
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