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Anti-Human TPM4 Polyclonal Antibody

Catalog #:   PHF61601 Specific References (15) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P67936
Overview

Catalog No.

PHF61601

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TPM4 (Met1-Ile248).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Tropomyosin alpha-4 chain, Tropomyosin-4, TM30p1, TPM4

Purification

Purified by antigen affinity column.

Accession

P67936

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with TPM4 antibody (PHF61601) at 1 µg/ml.

    Lane 1: A431 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: Jurkat cell lysate
    Lane 7: U87 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TPM4 antibody (PHF61601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References

Sarcomeric tropomyosin expression during human iPSC differentiation into cardiomyocytes., PMID:38470291

Association of anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis., PMID:37144941

Identification of a novel TPM4 isoform transcript and comparison to the expression of other tropomyosin isoforms in bovine cardiac and skeletal muscles., PMID:33824777

Sarcomeric TPM3 expression in human heart and skeletal muscle., PMID:32761805

Upregulation of tropomyosin alpha-4 chain in patients' saliva with oral squamous cell carcinoma as demonstrated by Phage display., PMID:31804537

Pulmonary inflammatory myofibroblastic tumor with TPM4-ALK translocation., PMID:29268561

Expression of various sarcomeric tropomyosin isoforms in equine striated muscles., PMID:28717602

Proteomic analysis and comparison of intra‑ and extracranial cerebral atherosclerosis responses to hyperlipidemia in rabbits., PMID:28677755

Identification, characterization, and expression of sarcomeric tropomyosin isoforms in zebrafish., PMID:27998020

Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans., PMID:27703814

Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery., PMID:26739486

Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule., PMID:24958154

LRRK2 guides the actin cytoskeleton at growth cones together with ARHGEF7 and Tropomyosin 4., PMID:24075941

Anti-endothelial cell antibodies in patients with cerebral small vessel disease., PMID:23030505

Characterization of the endothelial cell cytoskeleton following HLA class I ligation., PMID:22247778

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human TPM4 Polyclonal Antibody [PHF61601]
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