Catalog No.
PHA36501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PPP1R12A (Met1-Ser299).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, MBS, PPP1R12A, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1
Purification
Purified by antigen affinity column.
Accession
O14974
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Decreased Expression of MYPT1 Contributes to Tumor Angiogenesis and Poor Patient Prognosis in Human Prostate Cancer., PMID:29974831
Analysis of phosphorylation of the myosin-targeting subunit of myosin light chain phosphatase by Phos-tag SDS-PAGE., PMID:26864694
Using Quantitative Seroproteomics to Identify Antibody Biomarkers in Pancreatic Cancer., PMID:26842750
Toll-like receptor 2 mediates vascular contraction and activates RhoA signaling in vascular smooth muscle cells from STZ-induced type 1 diabetic rats., PMID:25600901
A key role for ROCK in TNF-α-mediated diabetic microvascular damage., PMID:23462755
Cross-talk between Rho-associated kinase and cyclic nucleotide-dependent kinase signaling pathways in the regulation of smooth muscle myosin light chain phosphatase., PMID:22948155
Synchronous phosphorylation of CPI-17 and MYPT1 is essential for inducing Ca(2+) sensitization in intestinal smooth muscle., PMID:22004286
Immunocytochemical evidence for PDBu-induced activation of RhoA/ROCK in human internal anal sphincter smooth muscle cells., PMID:21566015
Increased contractility of hepatic stellate cells in cirrhosis is mediated by enhanced Ca2+-dependent and Ca2+-sensitization pathways., PMID:21393429
ANP attenuates inflammatory signaling and Rho pathway of lung endothelial permeability induced by LPS and TNFalpha., PMID:19931545
Involvement of Rho kinase in endothelial barrier maintenance., PMID:17761936
Expression of RND proteins in human myometrium., PMID:16554414
For research use only. Not for human or drug use.
