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Anti-MAPRE1 Polyclonal Antibody

Catalog #:   PHH16501 Specific References (14) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q15691
Overview

Catalog No.

PHH16501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPRE1 (Ala2-Tyr268).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

EB1,Microtubule-associated protein RP/EB family member 1,MAPRE1,APC-binding protein EB1,End-binding protein 1

Purification

Purified by antigen affinity column.

Accession

Q15691

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with MAPRE1 antibody (PHH16501) at 0.36μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: Raji cell lysate
    Lane 3: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MAPRE1 antibody (PHH16501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 32 kDa
    Observed MW: 32 kDa
References

Proteomic identification of arginine-methylated proteins in colon cancer cells and comparison of messenger RNA expression between colorectal cancer and adjacent normal tissues., PMID:35081685

EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion., PMID:31112396

Combinatorial expression of microtubule-associated EB1 and ATIP3 biomarkers improves breast cancer prognosis., PMID:30368744

A centrosomal protein FOR20 regulates microtubule assembly dynamics and plays a role in cell migration., PMID:28694353

Negative regulation of EB1 turnover at microtubule plus ends by interaction with microtubule-associated protein ATIP3., PMID:26498358

MAPRE1 as a plasma biomarker for early-stage colorectal cancer and adenomas., PMID:26342024

ROS-mediated EB1 phosphorylation through Akt/GSK3β pathway: implication in cancer cell response to microtubule-targeting agents., PMID:24930764

Anti-migratory effect of vinflunine in endothelial and glioblastoma cells is associated with changes in EB1 C-terminal detyrosinated/tyrosinated status., PMID:23750272

GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migration., PMID:23236459

EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis., PMID:23001180

End-binding protein 1 controls signal propagation from the T cell receptor., PMID:22922463

Interactome-wide analysis identifies end-binding protein 1 as a crucial component for the speck-like particle formation of activated absence in melanoma 2 (AIM2) inflammasomes., PMID:22869553

Kif18B interacts with EB1 and controls astral microtubule length during mitosis., PMID:21737685

Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner., PMID:17318179

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-MAPRE1 Polyclonal Antibody [PHH16501]
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