Catalog No.
YVV20201
Expression system
E. coli
Species
Human rotavirus A
Protein length
Leu15-Leu173
Predicted molecular weight
21 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
E9BVI4
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Outer capsid protein VP4, VP4
Note
For research use only.
Rotavirus Spike Protein VP4 Mediates Viroplasm Assembly by Association to Actin Filaments., PMID:35938869
Phylogenetic analysis of the viral proteins VP4/VP7 of circulating human rotavirus strains in China from 2016 to 2019 and comparison of their antigenic epitopes with those of vaccine strains., PMID:36004332
Human rotavirus VP4 and VP7 genetic diversity and detection of GII norovirus in Ibadan as Nigeria introduces rotavirus vaccine., PMID:36138570
Generation and characterization of mouse monoclonal antibodies against the VP4 protein of group A human rotaviruses., PMID:36152816
The Association between Symptomatic Rotavirus Infection and Histo-Blood Group Antigens in Young Children with Diarrhea in Pretoria, South Africa., PMID:36560739
Global Infection Rate of Rotavirus C during 1980-2022 and Analysis of Critical Factors in the Host Range Restriction of Virus VP4., PMID:36560830
Characterization of Sialic Acid-Independent Simian Rotavirus Mutants in Viral Infection and Pathogenesis., PMID:36602365
Vaccine evaluation and genotype characterization in children infected with rotavirus in Qatar., PMID:36658331
A reverse genetics system for human rotavirus G2P[4]., PMID:36748482
Evaluation of Some Benzo[g]Quinazoline Derivatives as Antiviral Agents against Human Rotavirus Wa Strain: Biological Screening and Docking Study., PMID:36975526
Strain-Specific Interactions between the Viral Capsid Proteins VP4, VP7 and VP6 Influence Rescue of Rotavirus Reassortants by Reverse Genetics., PMID:36982745
Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals., PMID:36992447
A multidisciplinary approach to the identification of the protein-RNA connectome in double-stranded RNA virus capsids., PMID:37070191
Genotypic shift in rotavirus associated with neonatal outbreaks in Seoul, Korea., PMID:37253299
The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2., PMID:37905109
Genotype analysis of rotaviruses isolated from children during a phase III clinical trial with the hexavalent rotavirus vaccine in China., PMID:37972894
Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay., PMID:37991368
Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses., PMID:38140179
Identification of a novel intra-genotype reassortant G1P[8] rotavirus in Italy, 2021., PMID:38307378
Emergence and dissemination of equine-like G3P[8] rotavirus A in Brazil between 2015 and 2021., PMID:38451227
The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2., PMID:38574119
Recombinant bivalent subunit vaccine combining truncated VP4 from P[7] and P[23] induces protective immunity against prevalent porcine rotaviruses., PMID:38591886
Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys., PMID:38670845
VP4 Mutation Boosts Replication of Recombinant Human/Simian Rotavirus in Cell Culture., PMID:38675907
Antigenic epitope analysis of Pakistani G3 and G9 rotavirus strains compared to vaccine strains revealed multiple amino acid differences., PMID:38759540
Reverse Genetics of Murine Rotavirus: A Comparative Analysis of the Wild-Type and Cell-Culture-Adapted Murine Rotavirus VP4 in Replication and Virulence in Neonatal Mice., PMID:38793648
The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response., PMID:38850894
Specific binding sites on Rhesus rotavirus capsid protein dictate the method of endocytosis inducing the murine model of biliary atresia., PMID:38860860
Epidemiological Characteristics and Genotypic Features of Rotavirus and Norovirus in Jining City, 2021-2022., PMID:38932216
Human Rotaviruses of Multiple Genotypes Acquire Conserved VP4 Mutations during Serial Passage., PMID:38932271
Reverse vaccinology-based multi-epitope vaccine design against Indian group A rotavirus targeting VP7, VP4, and VP6 proteins., PMID:38960216
Chimeric Viruses Enable Study of Antibody Responses to Human Rotaviruses in Mice., PMID:39066309
A first report of rotavirus B from Zambian pigs leading to the discovery of a novel VP4 genotype P[9]., PMID:39449113
Whole Genome Sequences of the Wildtype AU-1 Rotavirus A Strain: The Prototype of the AU-1-like Genotype Constellation., PMID:39459863
New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit., PMID:39599844
Establishment of a Reverse Genetics System for Rotavirus Vaccine Strain LLR and Developing Vaccine Candidates Carrying VP7 Gene Cloned From Human Strains Circulating in China., PMID:39610277
Unusual G9P[4] Rotavirus Emerged After the Dynamic Changes in Rotavirus Genotypes From Equine-Like G3 to Typical Human G1/G3 in Indonesia., PMID:39670413
Epidemiological, molecular, and evolutionary characteristics of G1P[8] rotavirus in China on the eve of RotaTeq application., PMID:39717546
Epidemiology of viral gastroenteritis in children and genetic diversity of rotavirus strains in Kolkata, West Bengal after introduction of rotavirus vaccine., PMID:39731817
A matched case-control study of porcine group A and C rotaviruses in a swine farrowing production system., PMID:39765009
A single residue switch mediates the broad neutralization of Rotaviruses., PMID:39833145
Breast milk delivery of an engineered dimeric IgA protects neonates against rotavirus., PMID:39842610
14 years of rotavirus A surveillance: unusual dominance of equine-like G3P[8] genotype with DS-1-like genotype constellation after the pandemic, Belgium, 2009 to 2023., PMID:40156349
VP4-Specific IgA level as a correlate of neutralizing antibody and fecal shedding of porcine rotavirus infection., PMID:40179488
Truncated rotavirus VP4 proteins induce stronger protective immunity compared to P2 - VP8 in animal models., PMID:40194664
Immunoinformatics-driven design of a multi-epitope vaccine targeting neonatal rotavirus with focus on outer capsid proteins VP4 and VP7 and non structural proteins NSP2 and NSP5., PMID:40195509
Analysis of protein determinants of genotype-specific properties of group a rotaviruses using machine learning., PMID:40203739
Extended analyses of rotavirus C (RVC) G-types and P-types reveal new cut-off value for the G-types and reclassification of strains., PMID:40231817
Equine Rotavirus A Outbreaks in Ireland (2023-2024): An Epidemiological Investigation and Virus Genotyping., PMID:40284954
A rotavirus VP4 or VP7 monoreassortant panel identifies genotypes that are less susceptible to neutralization by systemic antibodies induced by vaccination or natural infection., PMID:40444468
For research use only. Not for human or drug use.
