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Anti-STAT4 Polyclonal Antibody

Catalog #:   PHG89501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q14765
Overview

Catalog No.

PHG89501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STAT4 (Tyr505-Ile737).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Signal transducer and activator of transcription 4,STAT4

Purification

Purified by antigen affinity column.

Accession

Q14765

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Immunocytochemistry/ Immunofluorescence
    STAT4 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with STAT4 (PHG89501) at 10.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with STAT4 antibody (PHG89501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
References

Triple positivity for autoantibodies in patients with rheumatoid arthritis is associated with a severe course of the disease but not with bone turnover markers., PMID:40485950

An activating Stat1 mutant disrupts normal STAT4 innate lymphocyte programs during viral infection., PMID:40446018

Systemic Sclerosis and Primary Biliary Cholangitis: A Comprehensive Review of Two Overlapping Rare Entities With Insights on Diagnostics and Management., PMID:40351968

Self-assembling sequentially administered tumor targeted Split IL-12p35 and p40 subunits to improve the therapeutic index of systemically delivered IL-12 therapy for cancer., PMID:40154091

Taming interleukin-12: Engineering of bispecific antibody-based IL-12 mimetics with biased agonism capacities., PMID:39981925

Borrelia burgdorferi sensu lato inhibits CIITA transcription through pSTAT3 activation and enhanced SOCS1 and SOCS3 expression leading to limited IFN-γ production., PMID:39879745

Interaction between interleukin-12 (IL-12) and its receptor (IL-12Rβ2) mediates CD4+ T cell subsets activation in flounder (Paralichthys olivaceus)., PMID:39743087

Irisin Improves Preeclampsia by Promoting Embryo Implantation and Vascular Remodeling., PMID:39540296

Genes deregulated in giant cell arteritis by Nanostring nCounter gene expression profiling in temporal artery biopsies., PMID:39317454

STAT4 and STAT6, their role in cellular and humoral immunity and in diverse human diseases., PMID:39188021

Anti-IL12p40 autoantibodies in a teenage girl with multiple recurrent abscesses., PMID:39098705

A Genome-Wide Association Study Suggests New Susceptibility Loci for Primary Antiphospholipid Syndrome., PMID:38973605

IL-12 drives the differentiation of human T follicular regulatory cells., PMID:38968337

Cordyceps protein alleviates renal injury by inhibiting T cell infiltration and Th1 cell differentiation in lupus nephritis mice., PMID:38943968

Hirsutella sinensis polysaccharides and Parabacteroides goldsteinii reduce lupus severity in imiquimod-treated mice., PMID:38901796

Machine learning-based prediction of rheumatoid arthritis with development of ACPA autoantibodies in the presence of non-HLA genes polymorphisms., PMID:38517871

A genome-wide association study suggests new susceptibility loci for primary antiphospholipid syndrome., PMID:38405993

STAT4 Gene Variant rs7574865 Is Associated with Rheumatoid Arthritis Activity and Anti-CCP Levels in the Western but Not in the Southern Population of Mexico., PMID:38397230

Evaluation of transcription factors and cytokine expressions of T-cell subsets in CD19 deficiency and their possible relationship with autoimmune disease., PMID:38095318

Hepatitis C virus modified sE2F442NYT as an antigen in candidate vaccine facilitates human immune cell activation., PMID:38084956

TOX, TWIST1, STAT4, and SATB1 protein expressions in early-stage mycosis fungoides., PMID:37932931

Biomarkers in Systemic Sclerosis: An Overview., PMID:37886934

Biomarkers in the Pathogenesis, Diagnosis, and Treatment of Systemic Sclerosis., PMID:37868834

Identification of lactate regulation pattern on tumor immune infiltration, therapy response, and DNA methylation in diffuse large B-cell lymphoma., PMID:37790933

Association Between Plasma Rituximab Concentration and the Risk of Major Relapse in Antineutrophil Cytoplasmic Antibody-Associated Vasculitides During Rituximab Maintenance Therapy., PMID:37134130

Preventing Surgery-Induced NK Cell Dysfunction Using Anti-TGF-β Immunotherapeutics., PMID:36498937

Myelin oligodendrocyte glycoprotein-associated disease is associated with BANK1, RNASET2 and TNIP1 polymorphisms., PMID:36054934

Non-HLA Gene Polymorphisms in the Pathogenesis of Type 1 Diabetes: Phase and Endotype Specific Effects., PMID:35812428

Association Study of Anticitrullinated Peptide Antibody Status with Clinical Manifestations and SNPs in Patients Affected with Rheumatoid Arthritis: A Pilot Study., PMID:35601739

Transcription Factors and Regulators Pathway-focused Genes Expression Analysis in Patients with Different Forms of Thyroid Pathology., PMID:35176984

The diagnostic role of PD-1+ CXCR5+ follicular helper CD8+ T cell in renal allograft dysfunction., PMID:34957609

Ubiquitinated Hepatitis D Antigen-Loaded Microvesicles Induce a Potent Specific Cellular Immune Response to Inhibit HDV Replication in Vivo., PMID:34908456

KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity., PMID:34836191

Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation., PMID:34471108

Abnormalities of the type I interferon signaling pathway in lupus autoimmunity., PMID:34340046

Association of the STAT4, CDKN1A, and IRF5 variants with risk of lupus nephritis and renal biopsy classification in patients in Vietnam., PMID:33687153

Myeloid-derived suppressor cell (MDSC) key genes analysis in rat anti-CD28-induced immune tolerance kidney transplantation., PMID:33532310

Type I Interferon-Activated STAT4 Regulation of Follicular Helper T Cell-Dependent Cytokine and Immunoglobulin Production in Lupus., PMID:33512094

STAT4 Is Largely Dispensable for Systemic Lupus Erythematosus-like Autoimmune- and Foreign Antigen-Driven Antibody-Forming Cell, Germinal Center, and Follicular Th Cell Responses., PMID:33446493

Anti-S100A4 Antibody Therapy Is Efficient in Treating Aggressive Prostate Cancer and Reversing Immunosuppression: Serum and Biopsy S100A4 as a Clinical Predictor., PMID:32999046

Lipocalin-2 Exacerbates Lupus Nephritis by Promoting Th1 Cell Differentiation., PMID:32646856

A Method of Assessment of Human Natural Killer Cell Phenotype and Function in Whole Blood., PMID:32508837

Cloning and Expression of the Tibetan Pig Interleukin-23 Gene and Its Promotion of Immunity of Pigs to PCV2 Vaccine., PMID:32466622

STAT4 sequence variant and elevated gene expression are associated with type 1 diabetes in Polish children., PMID:32425676

Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats., PMID:32308392

Immunological Fingerprints of Controllers Developing Neutralizing HIV-1 Antibodies., PMID:31995767

Genetics of Antiphospholipid Syndrome., PMID:31807905

The IL-12-STAT4 axis in the pathogenesis of human systemic lupus erythematosus., PMID:31762023

IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population., PMID:31570752

Neonatal T Follicular Helper Cells Are Lodged in a Pre-T Follicular Helper Stage Favoring Innate Over Adaptive Germinal Center Responses., PMID:31456798

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-STAT4 Polyclonal Antibody [PHG89501]
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