Catalog No.
KAC09601
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Trastuzumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Trastuzumab will be captured by immobilized Trastuzumab. After washing away any unbound substances, a biotin-labeled Trastuzumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Trastuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
3.13 - 200 ng/mL
Sensitivity
2.94 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
92.9 |
25.0 |
10.2 |
89.4 |
23.1 |
10.5 |
|
Standard deviation |
4.2 |
1.4 |
0.8 |
8.8 |
1.9 |
0.9 |
|
CV (%) |
4.5 |
5.6 |
7.9 |
9.9 |
8.3 |
8.2 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
timigutuzumab, 4D5-8, 4D5V8, Herceptin, rhuMab HER2,CAS: 180288-69-1
For research use only. Not for human or drug use.
