Anti-Daratumumab ELISA Kit

Catalog No.

KAD80801

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Daratumumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Daratumumab will be captured by immobilized Daratumumab. After washing away any unbound substances, a biotin-labeled Daratumumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Daratumumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Daratumumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

31.25 - 2,000 ng/mL

Sensitivity

18.96 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	833.0	201.1	85.2	835.9	210.9	89.5
Standard deviation	33.1	11.1	8.8	37.7	16.9	10.3
CV (%)	4.0	5.5	10.4	4.5	8.0	11.5

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

CAS: 945721-28-8

Association of plasma n-3 polyunsaturated fatty acid with gut mycobiome and implications for glucose homeostasis., PMID:40516019

In-situ Visualization of Level 3 LFPs Using a Lipophilic Red AIE Fluorescent Probe with a D-A Structure., PMID:40516016

Effect of hydrochemical conditions on transport of HFPO-DA in saturated quartz sand and limestone porous media., PMID:40515870

Context-dependent body size evolution in lacertid lizards: differential role of structural habitat and climate across radiations., PMID:40515787

'I'm Not a Bad Mom, I'm a Sick Mom': Using Photovoice to Examine Parental Recovery Narratives Given Institutional Constraints., PMID:40515630

Predicting Treatment Outcome in Congenital Adrenal Hyperplasia Using Urine Steroidomics and Machine Learning., PMID:40515610

Addressing the mean-variance relationship in spatially resolved transcriptomics data with spoon., PMID:40515599

Sibling-Focused Family Prevention With Latinx Siblings in Middle Childhood: A Randomized Clinical Trial Spanning the COVID-19 Pandemic., PMID:40515523

How Do Dental Professionals and Students Assess Orthodontic Case Complexity?, PMID:40515439

KansformerEPI: a deep learning framework integrating KAN and transformer for predicting enhancer-promoter interactions., PMID:40515390

Highly Selective Electrochemical Detection of Vitamin K1 (Phylloquinone) in Simulated Blood Serum Using Bimetallic Cu/Ni-MOF Decorated CNT Composite on Nickel Foam., PMID:40514770

Multi-trait/environment sparse genomic prediction using the SFSI R-package., PMID:40514769

Composition and rhythmic variations in the microbiome of Southwestern Atlantic corals., PMID:40514749

Secondary analysis of the EMPACT-MI trial reveals cardiovascular-kidney efficacy and safety of empagliflozin after acute myocardial infarction., PMID:40514435

Directing negative emotional states through parallel genetically-distinct basolateral amygdala pathways to ventral striatum subregions., PMID:40514388

Design, construction and characterization of laccase-xylanase chimeras by insertional fusion., PMID:40514177

Predictive value of preclinical models for CAR-T cell therapy clinical trials: a systematic review and meta-analysis., PMID:40514065

Experiences of Gender and Racial/Ethnic Discrimination in Sexual Healthcare Among Transgender and Nonbinary Young Adults of Color., PMID:40514048

Endoscopic submucosal dissection of early gastric cancer in the gastric fundus: challenges and techniques., PMID:40514029

In silico and in vitro screening of medicinal plants from Brazilian traditional medicine for anti-Helicobacter pylori activity., PMID:40514023

Association Between Preoperative Anemia and the Risk of Revision Following Total Knee Arthroplasty: A Multi-Institutional Retrospective Study., PMID:40513902

Local recurrence rates of horizontal margin-positive en bloc endoscopic submucosal dissection of colorectal neoplasia: a meta-analysis., PMID:40513803

Natural polysaccharide mediated hybrid intumescent flame retardant enables epoxy resin with high fire safety and mechanical performance., PMID:40513750

Exploring the protective mechanism of Lentinus edodes mycelium polysaccharide against AGEs-induced HUVECs damage: Insights from whole transcriptome sequencing and cell biology techniques., PMID:40513741

Oral delivery of tunable oxidation-responsive budesonide-loaded nanoparticles enhances inflammation modulation in intestinal colitis., PMID:40513667

Exposure to cadmium alters metabolic pathways in the hepatic proteome of a neotropical catfish., PMID:40513393

Nivolumab plus relatlimab in advanced melanoma: RELATIVITY-047 4-year update., PMID:40513285

Influence of Soil Characteristics on the Phytochemistry of Evergreen Ivy (Hedera helix L.) Leaves in Deciduous Forests., PMID:40513112

Investigating Cavity Quantum Electrodynamics-Enabled Endo/Exo-Selectivities in a Diels-Alder Reaction., PMID:40513107

Aberrant Right Vertebral Artery with Kommerell's Diverticulum: An Unique Lusoria Variant., PMID:40513104

Parasacral Transcutaneous Electrical Nerve Stimulation with Desmopressin Acetate for Treating Primary Monosymptomatic Enuresis: A Randomized Controlled Clinical Trial., PMID:40513022

Mycorrhizal symbioses and tree diversity in global forest communities., PMID:40512852

Eco-friendly degreasing adsorbent derived from oily scum and walnut shells for oilfield sewage treatment and industrial oils adsorption., PMID:40512703

Impact of Molecular Crowding on Accessibility of Telomeric Overhangs Forming Multiple G-Quadruplexes., PMID:40512671

Effects of concurrent training on maximal and explosive strength in trained individuals: Insights from the load-velocity relationship., PMID:40512632

Enhancing Monte Carlo Tree Search for Retrosynthesis., PMID:40512567

MicroMundo@Oeiras: Citizen Science promoting antibiotic stewardship, discovery of new antimicrobials and monitoring of soil resistance., PMID:40512524

The Impact of Yoga Practice on Health, Strength, and Respiratory Capacity in Portuguese Airforce Pilots: an Applied Psychophysiology and Biofeedback Approach., PMID:40512335

Optimized prism-based surface plasmon resonance sensor for malaria detection: role of 2D nanomaterials for an enhancement in sensitivity and accuracy., PMID:40512247

Protective Effect of Selegiline (R-deprenyl) in Aminoglycoside-Induced Hearing Loss., PMID:40512245

A multivariate analysis examining the relationship between sociodemographic differences and UK graduates' performance on postgraduate medical exams., PMID:40512226

[Theory-driven examination of available data on age(ing) and climate change: an explorative contribution]., PMID:40512225

From survival of irradiated mice to modern molecular insights: a seventy-year journey in radiobiology at the institute of biophysics, Czech academy of sciences., PMID:40512188

The challenging approach to the management of male partners of HPV-positive women., PMID:40511955

Comparing subjective effects and intoxication on simultaneous alcohol and cannabis use occasions relative to alcohol- or cannabis-only occasions., PMID:40511871

A Synthetic Route to Functionalized Thiopyran Derivatives via Domino Ring-Opening Cyclization (DROC) of Donor-Acceptor Cyclopropanes with Pyridinium Thiolates., PMID:40511864

Therapeutic Potential of PSC-derived Cell Transplantation in Parkinson's Disease: A Systematic Review and Meta-analysis of Preclinical Studies., PMID:40511801

Synthesis and Evaluation of Mesoporous Silica-Biopolymer-Based Bone Substitutes for Tissue Engineering., PMID:40511501

The Centriole Stability Assay: A Method to Investigate Mechanisms Involved in the Maintenance of the Centrosome Structure in Drosophila Cultured Cells., PMID:40511405

Characterization of Xiaoqu Qingxiangxing Baijiu by gas chromatography-olfactometry, quantitative measurements, aroma recombination, and omission experiments., PMID:40511332

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