Catalog No.
KAD80801
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Daratumumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Daratumumab will be captured by immobilized Daratumumab. After washing away any unbound substances, a biotin-labeled Daratumumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Daratumumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Daratumumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
31.25 - 2,000 ng/mL
Sensitivity
18.96 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
833.0 |
201.1 |
85.2 |
835.9 |
210.9 |
89.5 |
|
Standard deviation |
33.1 |
11.1 |
8.8 |
37.7 |
16.9 |
10.3 |
|
CV (%) |
4.0 |
5.5 |
10.4 |
4.5 |
8.0 |
11.5 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
CAS: 945721-28-8
For research use only. Not for human or drug use.
