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Anti-CAP1 Polyclonal Antibody

Catalog #:   PHF87301 Specific References (41) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q01518
Overview

Catalog No.

PHF87301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CAP1 (Ala38-Lys149).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Adenylyl cyclase-associated protein 1,CAP 1,CAP,CAP1

Purification

Purified by antigen affinity column.

Accession

Q01518

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CAP1 antibody (PHF87301) at 0.68μg/ml.

    Lane 1: K562 cell lysate
    Lane 2: Raji cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 51 kDa
    Observed MW: 58 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CAP1 antibody (PHF87301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 14 kDa
    Observed MW: 14 kDa
References

GPT-driven generation and biological activity evaluation of novel mRNA trinucleotide Cap1 analogs for mRNA vaccine or immunotherapy., PMID:40433678

Decoding the Resistin-CAP1 Pathway in Intermediate Monocytes Mediating Liver Allograft Rejection., PMID:40345627

Proprotein convertase subtilisin/kexin type 9 contributes to cisplatin-induced acute kidney injury by interacting with cyclase-associated protein 1 to promote megalin lysosomal degradation., PMID:40339661

PCSK9 stimulates Syk, PKCδ, and NF-κB, leading to atherosclerosis progression independently of LDL receptor., PMID:38555386

Expression of a SAG protein with a CAP domain from Eimeria necatrix and its role in invasion and immunoprotection., PMID:37931477

Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice., PMID:37876532

Uptake of Levilactobacillus brevis JCM 1059 by THP-1 Cells via Interaction between SlpB and CAP-1 Promotes Cytokine Production., PMID:36838212

Molecular interactions of PCSK9 with an inhibitory nanobody, CAP1 and HLA-C: Functional regulation of LDLR levels., PMID:36566984

Construction and Immunogenicity of Modified mRNA-Vaccine Variants Encoding Influenza Virus Antigens., PMID:34063689

Adipocytes Under Obese-Like Conditions Change Cell Cycle Distribution and Phosphorylation Profiles of Breast Cancer Cells: The Adipokine Receptor CAP1 Matters., PMID:33738261

The role of visfatin and resistin in an in vitro model of obesity-induced invasive liver cancer., PMID:33356858

Effects of tumor-specific CAP1 expression and body constitution on clinical outcomes in patients with early breast cancer., PMID:32560703

Use of a Cyclooxygenase-2 Inhibitor Does Not Inhibit Platelet Activation or Growth Factor Release From Platelet-Rich Plasma., PMID:28952781

Targeting Carcinoembryonic Antigen with DNA Vaccination: On-Target Adverse Events Link with Immunologic and Clinical Outcomes., PMID:27091407

Capsaicin-induced metabolic and cardiovascular autonomic improvement in an animal model of the metabolic syndrome., PMID:23968579

Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development., PMID:22526782

Ehrlichia chaffeensis TRP120 interacts with a diverse array of eukaryotic proteins involved in transcription, signaling, and cytoskeleton organization., PMID:21859857

Analysis of the response of Candida albicans cells to Silver(I)., PMID:20370363

Effects of physiologic agonists on canine whole blood flow cytometry assays of leukocyte-platelet aggregation and platelet activation., PMID:18405981

Identification and characterization of a type III secretion system in Chlamydophila psittaci., PMID:18275803

CAP2, cyclase-associated protein 2, is a dual compartment protein., PMID:17805484

Low TCR avidity and lack of tumor cell recognition in CD8(+) T cells primed with the CEA-analogue CAP1-6D peptide., PMID:17564703

Identification of Arabidopsis cyclase-associated protein 1 as the first nucleotide exchange factor for plant actin., PMID:17538023

Therapy of established tumors in a novel murine model transgenic for human carcinoembryonic antigen and HLA-A2 with a combination of anti-idiotype vaccine and CTL peptides of carcinoembryonic antigen., PMID:17363612

Effect of prolactin on carcinoembryonic antigen-specific cytotoxic T lymphocyte response induced by dendritic cells., PMID:15270849

Cloning and characterization of Cbl-associated protein splicing isoforms., PMID:12765336

Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human., PMID:12071850

CD8+ T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen Chlamydia trachomatis., PMID:11158611

Maize cap1 encodes a novel SERCA-type calcium-ATPase with a calmodulin-binding domain., PMID:10770930

Mammalian homolog of the yeast cyclase associated protein, CAP/Srv2p, regulates actin filament assembly., PMID:10658207

Use of monoclonal antibody in diagnosis of candidiasis caused by Candida albicans: detection of circulating aspartyl proteinase antigen., PMID:10548587

A Phase I study of active immunotherapy with carcinoembryonic antigen peptide (CAP-1)-pulsed, autologous human cultured dendritic cells in patients with metastatic malignancies expressing carcinoembryonic antigen., PMID:10389916

Production, characterization, and epitope mapping of a monoclonal antibody against aspartic proteinase of Candida albicans., PMID:10225850

In vitro induction of HLA-A2402-restricted and carcinoembryonic-antigen-specific cytotoxic T lymphocytes on fixed autologous peripheral blood cells., PMID:9769117

FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)., PMID:9184224

Type I Glanzmann's thrombasthenia in a Great Pyrenees dog., PMID:8885176

A platelet activation-specific monoclonal antibody that recognizes a receptor-induced binding site on canine fibrinogen., PMID:8817840

A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization., PMID:8552082

Differential expression of CAP and CAP2 in adult rat tissues., PMID:8522189

Mapping two functional domains of clathrin light chains with monoclonal antibodies., PMID:2435741

Production of anti-human thyroglobulin and anti-thyroid hormone antibodies in rabbits immunized with human thyroglobulin., PMID:4017976

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CAP1 Polyclonal Antibody [PHF87301]
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