Anti-MECP2 Antibody (N227/21)

Catalog No.

RHE84003

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

MeCp2, MeCp-2 protein, Methyl-CpG-binding protein 2, MECP2

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

N227/21

Jieyu Guben decoction alleviates combined allergic rhinitis and asthma syndrome by balancing Th17/Treg expression and restoring PPARD., PMID:40031093

Rett syndrome complicated by diabetes mellitus type 1., PMID:39907555

Response to Prof. Robert Colebunders' Letter submitted to JNS regarding: Spencer PS, Valdes Angues R, Palmer VS. Nodding syndrome: A role for environmental biotoxins that dysregulate MECP2 expression? J Neurol Sci. 2024 Jul 15;462:123077. doi: 10.1016/j.jns.2024.123077., PMID:39117466

Autoimmune and neuropsychiatric phenotypes in a Mecp2 transgenic mouse model on C57BL/6 background., PMID:38524134

In-depth comparison of Anc80L65 and AAV9 retinal targeting and characterization of cross-reactivity to multiple AAV serotypes in humans., PMID:37746244

Allele-Specific Epigenetic Regulation of FURIN Expression at a Coronary Artery Disease Susceptibility Locus., PMID:37443715

Mu opioid receptor expressing neurons in the rostral ventromedial medulla are the source of mechanical hypersensitivity induced by repeated restraint stress., PMID:37331575

Epigenetic modifier alpha-ketoglutarate modulates aberrant gene body methylation and hydroxymethylation marks in diabetic heart., PMID:37101286

A single-cell atlas reveals the heterogeneity of meningeal immunity in a mouse model of Methyl CpG binding protein 2 deficiency., PMID:36703978

The Cell Adhesion Molecule L1 Interacts with Methyl CpG Binding Protein 2 via Its Intracellular Domain., PMID:35408913

Epigenetic comparison of CHO hosts and clones reveals divergent methylation and transcription patterns across lineages., PMID:35028935

Anti-Semaphorin 4D Rescues Motor, Cognitive, and Respiratory Phenotypes in a Rett Syndrome Mouse Model., PMID:34502373

Long non‑coding RNA expression profiles identify lncRNA‑XLOC_I2_006631 as a potential novel blood biomarker for Hashimoto's thyroiditis., PMID:33125100

Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction., PMID:32807789

Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells., PMID:32718272

Fingolimod Modulates Dendritic Architecture in a BDNF-Dependent Manner., PMID:32349283

Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation., PMID:31606551

Electrochemical determination of the activity of DNA methyltransferase based on the methyl binding domain protein and a customized modular detector., PMID:30848391

Anti-MOG antibody encephalitis mimicking neurological deterioration in a case of Rett syndrome with MECP2 mutation., PMID:30227938

Methyl-CpG-binding protein 2 mediates antifibrotic effects in scleroderma fibroblasts., PMID:29760157

Altered microRNA, mRNA, and Protein Expression of Neurodegeneration-Related Biomarkers and Their Transcriptional and Epigenetic Modifiers in a Human Tau Transgenic Mouse Model in Response to Developmental Lead Exposure., PMID:29614648

Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world., PMID:28955766

Antibiotic Prophylaxis, Immunoglobulin Substitution and Supportive Measures Prevent Infections in MECP2 Duplication Syndrome., PMID:28938254

Identification of autism-related MECP2 mutations by whole-exome sequencing and functional validation., PMID:28785396

Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells., PMID:28754949

Zebrafish Mecp2 is required for proper axonal elongation of motor neurons and synapse formation., PMID:28371371

Promoter CpG-Site Methylation of the KAI1 Metastasis Suppressor Gene Contributes to Its Epigenetic Repression in Prostate Cancer., PMID:27813113

IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics., PMID:27113293

Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands., PMID:27064487

Rett syndrome: An autoimmune disease?, PMID:26807990

Methyl-CpG Binding Protein 2 (Mecp2) Regulates Sensory Function Through Sema5b and Robo2., PMID:26733807

Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2., PMID:26235378

MeCP2 in the enteric nervous system., PMID:26031318

Methyl CpG binding protein 2 deficiency enhances expression of inflammatory cytokines by sustaining NF-κB signaling in myeloid derived cells., PMID:26004152

Defects During Mecp2 Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms., PMID:25979088

Infectious and immunologic phenotype of MECP2 duplication syndrome., PMID:25721700

Perinatal nicotine exposure suppresses PPARγ epigenetically in lung alveolar interstitial fibroblasts., PMID:25661292

Epigenetic regulation contributes to urocortin-enhanced midbrain dopaminergic neuron differentiation., PMID:25641682

Thyroid function in Rett syndrome., PMID:25614013

Transcriptional Regulation of Brain-Derived Neurotrophic Factor (BDNF) by Methyl CpG Binding Protein 2 (MeCP2): a Novel Mechanism for Re-Myelination and/or Myelin Repair Involved in the Treatment of Multiple Sclerosis (MS)., PMID:25579386

Immune dysfunction in Rett syndrome patients revealed by high levels of serum anti-N(Glc) IgM antibody fraction., PMID:25389532

Isoform-specific anti-MeCP2 antibodies confirm that expression of the e1 isoform strongly predominates in the brain., PMID:24555100

Increased binding of MeCP2 to the GAD1 and RELN promoters may be mediated by an enrichment of 5-hmC in autism spectrum disorder (ASD) cerebellum., PMID:24448211

Mice with an isoform-ablating Mecp2 exon 1 mutation recapitulate the neurologic deficits of Rett syndrome., PMID:24352790

Ultrasensitive electrochemical immunoassay for DNA methyltransferase activity and inhibitor screening based on methyl binding domain protein of MeCP2 and enzymatic signal amplification., PMID:23708816

Functional characterization of the MECP2/IRAK1 lupus risk haplotype in human T cells and a human MECP2 transgenic mouse., PMID:23428850

MeCP2 modulates the canonical Wnt pathway activation by targeting SFRP4 in rheumatoid arthritis fibroblast-like synoviocytes in rats., PMID:23200852

Novel MeCP2 isoform-specific antibody reveals the endogenous MeCP2E1 expression in murine brain, primary neurons and astrocytes., PMID:23185431

Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin., PMID:23149574

Further evidence of subphenotype association with systemic lupus erythematosus susceptibility loci: a European cases only study., PMID:23049788

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