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Anti-ASFV p54/pE183L Polyclonal Antibody

Catalog #:   PVV16001 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: R4S7F0
Overview

Catalog No.

PVV16001

Species reactivity

African swine fever virus (ASFV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV p54/pE183L (Met1-Leu133).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

Inner membrane protein p54, pE183L, E183L

Concentration

0.71 mg/ml

Purification

Purified by antigen affinity column.

Accession

R4S7F0

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with p54/pE183L antibody (PVV16001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
References

The Novel Antigenic Epitopes of African Swine Fever Virus Inner Membrane p54 Protein Revealed by Monoclonal Antibodies., PMID:40362108

Encapsulin nanoparticle-conjugated p54 protein boosts immune responses against African swine fever virus., PMID:40324494

Development and efficacy of a novel mRNA cocktail for the delivery of African swine fever virus antigens and induction of immune responses., PMID:40298440

Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs., PMID:40250107

Evaluation of the Immunogenicity of a Pool of Recombinant Lactococcus lactis Expressing Eight Antigens of African Swine Fever Virus in a Mouse Model., PMID:40005900

A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs., PMID:39852784

p54-Fc-Labeled Gold Nanoparticle-Based Lateral Flow Strip-Assisted Portable Devices for Rapid and Quantitative Point-of-Care Detection of ASFV Antibodies., PMID:39852075

Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever., PMID:39599534

A Nanobody-based TRIM-away targets the intracellular protein degradation of African swine fever virus., PMID:39488886

Establishment of an ELISA Based on a Recombinant Antigenic Protein Containing Multiple Prominent Epitopes for Detection of African Swine Fever Virus Antibodies., PMID:38792774

Preparation and epitope analysis of monoclonal antibodies against African swine fever virus DP96R protein., PMID:38734611

A vesicular stomatitis virus-based African swine fever vaccine prototype effectively induced robust immune responses in mice following a single-dose immunization., PMID:38249478

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54., PMID:38203508

Retrospective Multi-Locus Sequence Analysis of African Swine Fever Viruses by "PACT" Confirms Co-Circulation of Multiple Outbreak Strains in Uganda., PMID:38200802

ASFV epitope mapping by high density peptides microarrays., PMID:38029799

Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines., PMID:38006019

A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs., PMID:38004769

A blocking ELISA based on virus-like nanoparticles chimerized with an antigenic epitope of ASFV P54 for detecting ASFV antibodies., PMID:37968284

Establishment and characterization of a novel indirect ELISA method based on ASFV antigenic epitope-associated recombinant protein., PMID:37865977

Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens., PMID:37720232

Molecular Signatures in Swine Innate and Adaptive Immune Responses to African Swine Fever Virus Antigens p30/p54/CD2v Expressed Using a Highly Efficient Semliki Forest Virus Replicon System., PMID:37298266

Screening and identification of the dominant antigens of the African swine fever virus., PMID:37215478

Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool., PMID:37191526

A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies., PMID:37112846

ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice., PMID:36965632

Dynamics of Serological and Mucosal Antibody Responses against African Swine Fever Viruses in Experimentally Infected Pigs., PMID:40303747

Indirect ELISA Using Multi-Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs., PMID:36560663

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus., PMID:36366433

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus., PMID:36189357

Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus., PMID:36038241

Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate., PMID:36016353

Comparison of mucosal immune responses to African swine fever virus antigens intranasally delivered with two different viral vectors., PMID:35849881

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay., PMID:35698809

HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies., PMID:35612629

Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes., PMID:35498728

Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS)., PMID:34815032

Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions., PMID:34799123

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide., PMID:34715492

Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA., PMID:34638027

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum., PMID:34454001

Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens., PMID:34399310

Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54., PMID:33737177

Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis., PMID:33562314

Unpicking the Secrets of African Swine Fever Viral Replication Sites., PMID:33429879

Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine., PMID:32915442

Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip., PMID:32887174

Epitope mapping of African swine fever virus (ASFV) structural protein, p54., PMID:32004574

Evidence for the presence of African swine fever virus in apparently healthy pigs in South-Kivu Province of the Democratic Republic of Congo., PMID:31902515

DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain., PMID:30696015

Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus., PMID:30367292

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-ASFV p54/pE183L Polyclonal Antibody [PVV16001]
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