Catalog No.
KAC09651
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Pertuzumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD340 and then pipetted into the wells. Anti-Pertuzumab Neutralizing Antibody in the sample competitively binds to Pertuzumab with the biotin-labeled Human CD340. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pertuzumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Pertuzumab Neutralizing Antibody concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
50 - 800 ng/mL
Sensitivity
41.4 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
364.3 |
100.4 |
46.8 |
310.0 |
122.5 |
69.2 |
|
Standard deviation |
18.5 |
6.4 |
9.1 |
19.2 |
14.0 |
12.9 |
|
CV (%) |
5.1 |
6.3 |
19.4 |
6.2 |
11.4 |
18.6 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
OMNITARG, rhuMAB 2C4, CAS: 380610-27-5
For research use only. Not for human or drug use.
