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Anti-CD81 Polyclonal Antibody

Catalog #:   PHF34001 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P60033
Overview

Catalog No.

PHF34001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD81 (Phe113-Lys201).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

CD81 antigen,TSPAN28,Target of the antiproliferative antibody 1,Tspan-28,Tetraspanin-28,CD81,TAPA1,26 kDa cell surface protein TAPA-1

Concentration

0.51 mg/ml

Purification

Purified by antigen affinity column.

Accession

P60033

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD81 antibody (PHF34001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 12 kDa
    Observed MW: 12 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with CD81 antibody (PHF34001) at 1 μg/ml.

    Lane 1: Ramos
    Lane 2: C6
    Lane 3: PC12

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 26 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human testis stained for CD81 with PHF34001.
References

Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity., PMID:40500295

Ultrasound-triggered targeted delivery of engineered ADSCs-derived exosomes with high SDF-1α levels to promote cardiac repair following myocardial infarction., PMID:40460966

An Extracellular Vesicle (EV) Paper Strip for Rapid and Convenient Estimation of EV Concentration., PMID:40422033

Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma., PMID:40345522

[Efficient capture and proteomics analysis of urinary extracellular vesicles by affinity purification]., PMID:40331614

Extracellular Vesicle Marker Changes Associated With Disease Activity in Relapsing-Remitting Multiple Sclerosis., PMID:40300121

Glycoengineering of the hepatitis C virus E2 glycoprotein leads to improved biochemical properties and enhanced immunogenicity., PMID:40291659

Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle Counting., PMID:40166220

Nanoscopic Profiling of Small Extracellular Vesicles via High-Speed Atomic Force Microscopy (HS-AFM) Videography., PMID:40139685

Next Generation Aqueous Two-Phase System for Gentle, Effective, and Timely Extracellular Vesicle Isolation and Transcriptomic Analysis., PMID:40108918

Isolation of Urinary Extracellular Vesicles (EVs) via Hydrophobic Interaction Chromatography Using a Nylon-6 Capillary-Channeled Polymer (C-CP) Fiber Column., PMID:39933961

EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device., PMID:39918033

Tetraspanin CD81 serves as a functional entry factor for porcine circovirus type 2 infection., PMID:39745447

EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations., PMID:39692115

A novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma., PMID:39607297

Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation., PMID:39574085

Multi-omic characteristics of longitudinal immune profiling after breakthrough infections caused by Omicron BA.5 sublineages., PMID:39536392

Investigating the effect of immunomagnetic separation on the immunophenotype and viability of plasma cells in plasma cell disorders., PMID:39493694

Citrullination Accompanies the Development of Carotid Atherosclerotic Plaques., PMID:39421992

Comparison of nanoimaging and nanoflow based detection of extracellular vesicles at a single particle resolution., PMID:39416671

Enhancing Extracellular Vesicle Detection via Cotargeting Tetraspanin Biomarkers., PMID:39360503

Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle Nanoscopy., PMID:39342336

Sensitive phenotyping of serum extracellular vesicles on a SERS-microfluidic platform for early-stage clinical diagnosis of ovarian carcinoma., PMID:39260102

Pharmacologic HIF stabilization activates costimulatory receptor expression to increase antitumor efficacy of adoptive T cell therapy., PMID:39196939

Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response., PMID:39176090

Hepatitis C virus chronicity and oncogenic potential: Vaccine development progress., PMID:39167987

CD81 fusion alters SARS-CoV-2 Spike trafficking., PMID:39140770

Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer., PMID:39140467

Urinary Extracellular Vesicles as a Readily Available Biomarker Source: A Simplified Stratification Method., PMID:39125575

Exosomal membrane proteins analysis using a silicon nanowire field effect transistor biosensor., PMID:39002259

Immunoassays for Extracellular Vesicle Detection via Transmembrane Proteins Using Surface Plasmon Resonance Biosensors., PMID:38912608

Circulating small extracellular vesicles in Alzheimer's disease: a case-control study of neuro-inflammation and synaptic dysfunction., PMID:38902659

Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease., PMID:38898558

Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity., PMID:38896451

Impact of brain organoid-derived sEVs on metastatic adaptation and invasion of breast carcinoma cells through a microphysiological system., PMID:38888211

Computational Study on the Wealth Conformations of Hepatitis C Virus Envelope Glycoprotein 2 and Role of Its Glycan Coat., PMID:38861660

Alterations in B and NK cells highly correlate with disease severity in children with COVID-19., PMID:38813014

Immune checkpoint inhibitors for POLE or POLD1 proofreading-deficient metastatic colorectal cancer., PMID:38777726

High-throughput proteomic analysis of extracellular vesicles from saliva by chemical probe-based array., PMID:38772652

The antitumor effect of extracellular vesicles derived from cytokine-activated CD8+ T cells., PMID:38753658

CD71: Role in permafrost immunity., PMID:38711995

Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed., PMID:38659349

Spectroscopic insight into breast cancer: profiling small extracellular vesicles lipids via infrared spectroscopy for diagnostic precision., PMID:38654096

[Exosomes' role in intercellular interactions in different variants of lung injury in fatal cases of COVID-19]., PMID:38591903

A molecular docking exploration of the large extracellular loop of tetraspanin CD81 with small molecules., PMID:38584777

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes., PMID:38520356

Isoprinosine as a foot-and-mouth disease vaccine adjuvant elicits robust host defense against viral infection through immunomodulation., PMID:38510965

Faster clinical decisions in B-cell acute lymphoblastic leukaemia: A single flow cytometric 12-colour tube improves diagnosis and minimal residual disease follow-up., PMID:38432068

Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche., PMID:38414005

Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker., PMID:38400284

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CD81 Polyclonal Antibody [PHF34001]
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