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Anti-LOXL3 Polyclonal Antibody

Catalog #:   PHF25801 Specific References (8) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P58215
Overview

Catalog No.

PHF25801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LOXL3 (Thr519-Asn726).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Lysyl oxidase homolog 3,LOXL3,LOXL,Lysyl oxidase-like protein 3

Purification

Purified by antigen affinity column.

Accession

P58215

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LOXL3 antibody (PHF25801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 26 kDa
  • Immunocytochemistry/ Immunofluorescence
    LOXL3 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with LOXL3 (PHF25801) at 7.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Lysyl Oxidase (LOX) Family Members: Rationale and Their Potential as Therapeutic Targets for Liver Fibrosis., PMID:32176358

Hypoxia triggers angiogenesis by increasing expression of LOX genes in 3-D culture of ASCs and ECs., PMID:28189640

Insights in the etiopathology of galactosyltransferase II (GalT-II) deficiency from transcriptome-wide expression profiling of skin fibroblasts of two sisters with compound heterozygosity for two novel B3GALT6 mutations., PMID:28649518

A multimarker panel for circulating tumor cells detection predicts patient outcome and therapy response in metastatic colorectal cancer., PMID:24752533

Changes in lysyl oxidase (LOX) distribution and its decreased activity in keratoconus corneas., PMID:23041260

Modulation of lysyl oxidase-like 2 enzymatic activity by an allosteric antibody inhibitor., PMID:20439985

Reciprocal regulation of LOX and LOXL2 expression during cell adhesion and terminal differentiation in epidermal keratinocytes., PMID:19394199

A molecular role for lysyl oxidase-like 2 enzyme in snail regulation and tumor progression., PMID:16096638

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-LOXL3 Polyclonal Antibody [PHF25801]
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