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Anti-PRKAA2 Polyclonal Antibody

Catalog #:   PHF04001 Specific References (14) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P54646
Overview

Catalog No.

PHF04001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRKAA2 (Met1-Arg552).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PRKAA2,Acetyl-CoA carboxylase kinase,AMPK subunit alpha-2,ACACA kinase,AMPK,AMPK2,Hydroxymethylglutaryl-CoA reductase kinase,5'-AMP-activated protein kinase catalytic subunit alpha-2,HMGCR kinase

Purification

Purified by antigen affinity column.

Accession

P54646

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PRKAA2 antibody (PHF04001) at 0.43μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: A2780 cell lysate
    Lane 3: K562 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 63 kDa
    Observed MW: 66 kDa
  • Immunocytochemistry/ Immunofluorescence
    PRKAA2 in SKOV3 Cell Line.
    The SKOV3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PRKAA2 (PHF04001) at 8.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PRKAA2 antibody (PHF04001) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 65 kDa
    Observed MW: 65 kDa
References

4D label-free quantitative proteomic and phosphoproteomic profiling of the kidney in rat models of antimyeloperoxidase-associated vasculitis., PMID:40201969

Exploration and Identification of Vitamin D and Related Genes as Potential Biomarkers for Colorectal Tumors., PMID:39872437

AMPKα2 promotes tumor immune escape by inducing CD8+ T-cell exhaustion and CD4+ Treg cell formation in liver hepatocellular carcinoma., PMID:38424484

Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome., PMID:31398040

Autoantibody against β1-adrenoceptor promotes the differentiation of natural regulatory T cells from activated CD4+ T cells by up-regulating AMPK-mediated fatty acid oxidation., PMID:30770790

CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells., PMID:25590814

AMPK/TSC2/mTOR-signaling intermediates are not necessary for LKB1-mediated nuclear retention of PTEN tumor suppressor., PMID:21123367

Ablation of AMP-activated protein kinase alpha1 and alpha2 from mouse pancreatic beta cells and RIP2.Cre neurons suppresses insulin release in vivo., PMID:20221584

Activation of AMP-activated protein kinase by vascular endothelial growth factor mediates endothelial angiogenesis independently of nitric-oxide synthase., PMID:20129920

Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle., PMID:19923418

Detection of AMP-activated protein kinase in human sera by immuno-isoelectric focusing., PMID:19818790

Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing SLC2A4 (GLUT4) in the adipose tissue by AMP-activated protein kinase activation., PMID:18719073

Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle., PMID:14559719

Metabolic and mitogenic signal transduction in human skeletal muscle after intense cycling exercise., PMID:12527721

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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For research use only. Not for human or drug use.

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Anti-PRKAA2 Polyclonal Antibody [PHF04001]
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