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Anti-Human POU4F2 Polyclonal Antibody

Catalog #:   PHG30501 Specific References (9) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q12837
Overview

Catalog No.

PHG30501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POU4F2 (Gly246-Lys336).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

POU domain, class 4, transcription factor 2, POU4F2, Brn-3B, BRN3B, Brain-3B, Brain-specific homeobox/POU domain protein 3B

Purification

Purified by antigen affinity column.

Accession

Q12837

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with POU4F2 antibody (PHG30501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
References

nGnG Amacrine Cells and Brn3b-negative M1 ipRGCs are Specifically Labeled in the ChAT-ChR2-EYFP Mouse., PMID:32049344

Adult mice transplanted with embryonic retinal progenitor cells: new approach for repairing damaged optic nerves., PMID:23170059

Differential expression of Brn3 transcription factors in intrinsically photosensitive retinal ganglion cells in mouse., PMID:21935940

Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse., PMID:19459208

Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2., PMID:18460603

Analysis of optic nerve stroke by retinal Bex expression., PMID:16541015

Elevated expression of the Brn-3a and Brn-3b transcription factors in systemic lupus erythematosus correlates with antibodies to Brn-3 and overexpression of Hsp90., PMID:15818685

Delayed expression of the Crx gene and photoreceptor development in the Chx10-deficient retina., PMID:14744875

The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons., PMID:7623109

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human POU4F2 Polyclonal Antibody [PHG30501]
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