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Anti-ECE1 Polyclonal Antibody

Catalog #:   PHE38401 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P42892
Overview

Catalog No.

PHE38401

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ECE1 (Leu469-Glu721).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ECE1,Endothelin-converting enzyme 1,ECE-1

Purification

Purified by antigen affinity column.

Accession

P42892

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ECE1 antibody (PHE38401) at 1μg/ml.

    Lane 1: A549 cell lysate
    Lane 2: HUVEC cell lysate
    Lane 3: NIH3T3 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: H1299 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 88 kDa
    Observed MW: 125 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse kidney stained for ECE1 with PHE38401.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ECE1 antibody (PHE38401) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different Candida albicans Extracellular Vesicles., PMID:39796100

Comprehensive analysis of the endothelin system in the kidneys of mice, rats, and humans., PMID:38904098

The hyphal-specific toxin candidalysin promotes fungal gut commensalism., PMID:38448595

Nanobody-mediated neutralization of candidalysin prevents epithelial damage and inflammatory responses that drive vulvovaginal candidiasis pathogenesis., PMID:38349176

Dynamic Glycoprotein Hyposialylation Promotes Chemotherapy Evasion and Metastatic Seeding of Quiescent Circulating Tumor Cell Clusters in Breast Cancer., PMID:37272843

The Candida albicans toxin candidalysin mediates distinct epithelial inflammatory responses through p38 and EGFR-ERK pathways., PMID:35380879

IL-1α released from oral epithelial cells upon candidalysin exposure initiates an early innate epithelial response., PMID:33038250

Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis., PMID:27794420

Glial Endothelin-1 Regulates Retinal Blood Flow During Hyperoxia in Cats., PMID:27654423

TIMAP-protein phosphatase 1-complex controls endothelin-1 production via ECE-1 dephosphorylation., PMID:26806547

An arterial-specific enhancer of the human endothelin converting enzyme 1 (ECE1) gene is synergistically activated by Sox17, FoxC2, and Etv2., PMID:25179465

Intracrine endothelin signaling evokes IP3-dependent increases in nucleoplasmic Ca²⁺ in adult cardiac myocytes., PMID:23756157

Fludarabine and cladribine induce changes in surface proteins on human B-lymphoid cell lines involved with apoptosis, cell survival, and antitumor immunity., PMID:22839105

Role of Protein Kinase C in Endothelin Converting Enzyme-1 trafficking and shedding from endothelial cells., PMID:20558134

Increased endothelial exocytosis and generation of endothelin-1 contributes to constriction of aged arteries., PMID:20522806

Transdifferentiation potentiality of human Wharton's jelly stem cells towards vascular endothelial cells., PMID:20143331

Effect of smoking on rat basilar artery: correlation with inducible nitric oxide synthase and endothelin converting enzyme-1., PMID:19847761

Endothelin-1, endothelin converting enzyme-1 and endothelin receptors in the porcine corpus luteum., PMID:19783117

Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage responses, morphogenesis, and virulence., PMID:19252121

Functional and molecular characterization of the endothelin system in retinal arterioles., PMID:19151386

Transducible P11-CNTF rescues the learning and memory impairments induced by amyloid-beta peptide in mice., PMID:18644361

Endothelin-converting enzyme-1 degrades internalized somatostatin-14., PMID:18276747

Immunocapture-based fluorometric assay for the measurement of insulin-degrading enzyme activity in brain tissue homogenates., PMID:18221786

Expression and localization of human endothelin-converting enzyme-1 isoforms in symptomatic atherosclerotic disease and saphenous vein., PMID:16741001

Identification of a novel alternatively spliced variant endothelin converting enzyme-1 lacking a transmembrane domain., PMID:16740988

Expression and localization of endothelin-converting enzyme-1 isoforms in human endothelial cells., PMID:16740987

Phosphorylation of endothelin converting enzyme-1 isoforms: relevance to subcellular localization., PMID:16740986

The endothelin system and renin in human fetal membranes., PMID:16375820

Histochemical distribution of endothelin-converting enzyme subtypes in Podarcis sicula (Squamata, Lacertidae) tissues., PMID:16191167

Endothelin-converting enzyme-1, abundance of isoforms a-d and identification of a novel alternatively spliced variant lacking a transmembrane domain., PMID:16186113

Increased immunoreactivities against endothelin-converting enzyme-1 and monocyte chemotactic protein-1 in hepatic stellate cells of rat fibrous liver induced by thioacetamide., PMID:16170464

Vascular endothelial growth factor system in the cow oviduct: a possible involvement in the regulation of oviductal motility and embryo transport., PMID:16155957

Localization of the endothelin system in human diffuse astrocytomas., PMID:16007684

Endothelin-converting enzyme-1 is expressed in human cerebral cortex and protects against Alzheimer's disease., PMID:15340356

Endothelin and subarachnoid hemorrhage-induced cerebral vasospasm: pathogenesis and treatment., PMID:15279581

Cellular distribution of the endothelin system in the human brain., PMID:15121213

Increased expression of endothelin-converting enzyme-1 in failing human myocardium., PMID:12193094

An immunohistochemical study of endothelin-1 converting enzyme in the human eye., PMID:12187489

Transcriptional mechanism of protein kinase C-induced isoform-specific expression of the gene for endothelin-converting enzyme-1 in human endothelial cells., PMID:11723240

Biochemical characterization of endothelin-converting enzyme-1alpha in cultured skin-derived cells and its postulated role in the stimulation of melanogenesis in human epidermis., PMID:11723113

Endothelin-1 pathway in human alveolar epithelial cell line A549 and human umbilical vein endothelial cells., PMID:11360683

Enhanced expression of renal endothelin-converting enzyme-1 and endothelin-A-receptor mRNA in rats with interstitial fibrosis following ureter ligation., PMID:11078391

Functional significance of the isoforms of endothelin-converting enzyme-1., PMID:11078326

Expression of the endothelin-converting enzyme-1 isoforms in endothelial cells., PMID:11078323

Immunohistochemical distribution of the endothelin-converting enzyme-1 in the rat hypothalamo-pituitary axis., PMID:10771167

Immunohistochemical localization of ECE-1 in the human placenta., PMID:10736246

Sequencing, expression and biochemical characterization of the Porphyromonas gingivalis pepO gene encoding a protein homologous to human endothelin-converting enzyme., PMID:10571076

A fourth isoform of endothelin-converting enzyme (ECE-1) is generated from an additional promoter molecular cloning and characterization., PMID:10491078

Two di-leucine-based motifs account for the different subcellular localizations of the human endothelin-converting enzyme (ECE-1) isoforms., PMID:10462527

Identification of endothelin converting enzyme-1 in human non-pigmented ciliary epithelial cells., PMID:10433854

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-ECE1 Polyclonal Antibody [PHE38401]
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