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Anti-WNT5A Polyclonal Antibody

Catalog #:   PME30701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P22725
Overview

Catalog No.

PME30701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse WNT5A (Ile62-Lys380).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Protein Wnt-5a,WNT5A

Purification

Purified by antigen affinity column.

Accession

P22725

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with WNT5A antibody (PME30701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 38 kDa
    Observed MW: 38 kDa
References

The impact of autoimmune thyroid disease on cognitive and psychiatric disorders: focus on clinical, pre-clinical and molecular studies., PMID:40445744

Cancer-associated fibroblasts promote growth and dissemination of esophageal squamous cell carcinoma cells by secreting WNT family member 5A., PMID:39954174

Non-Canonical Wnt16 and microRNA-145 Mediate the Response of Human Bone Marrow Stromal Cells to Additively Manufactured Porous 3-Dimensional Biomimetic Titanium-Aluminum-Vanadium Constructs., PMID:39937002

Targeting ROR2 homooligomerization disrupts ROR2-dependent signaling and suppresses stem-like cell properties of human breast adenocarcinoma., PMID:39829682

Identification of a novel anti-ROR1 nanobody through phage display and its biochemical characterization., PMID:39645564

Neutrophil pyroptosis regulates corneal wound healing and post-injury neovascularisation., PMID:39496510

Structural insights into Frizzled3 through nanobody modulators., PMID:39174501

Requirement of a Wnt5A-microbiota axis in the maintenance of gut B-cell repertoire and protection from infection., PMID:39140737

9-O-monoethyl succinate berberine effectively blocks the PI3K/AKT signaling pathway by targeting Wnt5a protein in inhibiting osteosarcoma growth., PMID:39047413

Receptor tyrosine kinase-like orphan receptor serves as a potential target in cancer immunotherapy., PMID:38973261

How receptor tyrosine kinase-like orphan receptor 1 meets its partners in chronic lymphocytic leukemia., PMID:38949887

Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling., PMID:38780011

Synovial osteoclastogenesis mediated by chondrocyte-secreted TNFα promotes TMJ condylar resorption., PMID:38720613

Wnt16 Increases Bone-to-Implant Contact in an Osteopenic Rat Model by Increasing Proliferation and Regulating the Differentiation of Bone Marrow Stromal Cells., PMID:38517621

The Notch1 signaling pathway directly modulates the human RANKL-induced osteoclastogenesis., PMID:38040752

Wnt1 induces osteoblastic changes in a well-established osteolytic skeletal metastatic model derived from breast cancer., PMID:37840014

Ehrlichia Wnt SLiM ligand mimic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis., PMID:37530530

Stricturing Crohn's Disease Single-Cell RNA Sequencing Reveals Fibroblast Heterogeneity and Intercellular Interactions., PMID:37507073

Ehrlichia Wnt short linear motif ligand mimetic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis., PMID:36945589

WNT5A-ROR2 axis mediates VEGF dependence of BRAF mutant melanoma., PMID:36539575

ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer., PMID:36476423

Periodontal ligaments enhance neurite outgrowth in trigeminal ganglion neurons through Wnt5a production induced by mechanical stimulation., PMID:36374171

WNT5A drives interleukin-6-dependent epithelial-mesenchymal transition via the JAK/STAT pathway in keloid pathogenesis., PMID:36225328

Pep27 Mutant Immunization Inhibits Caspase-14 Expression to Alleviate Inflammatory Bowel Disease via Treg Upregulation., PMID:36144473

Frequently used antiemetic agent dexamethasone enhances the metastatic behaviour of select breast cancer cells., PMID:36107918

Wnt5a/β-catenin axis is involved in the downregulation of AT2 lineage by PAI-1., PMID:36098461

Fatty Acid Signaling Impacts Prostate Cancer Lineage Plasticity in an Autocrine and Paracrine Manner., PMID:35884514

Berberine retarded the growth of gastric cancer xenograft tumors by targeting hepatocyte nuclear factor 4α., PMID:35582103

Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas., PMID:35551459

In utero exposure to maternal anti-aquaporin-4 antibodies alters brain vasculature and neural dynamics in male mouse offspring., PMID:35442708

Memory B-cell like chronic lymphocytic leukaemia is associated with specific methylation profile of WNT5A promoter and undetectable expression of WNT5A gene., PMID:35333703

WNT5A Interacts With FZD5 and LRP5 to Regulate Proliferation and Self-Renewal of Endometrial Mesenchymal Stem-Like Cells., PMID:35295855

Aberrantly expressed Wnt5a in nurse-like cells drives resistance to Venetoclax in chronic lymphocytic leukemia., PMID:35210425

TNF-α Activating Osteoclasts in Patients with Psoriatic Arthritis Enhances the Recruitment of Osteoclast Precursors: A Plausible Role of WNT5A-MCP-1 in Osteoclast Engagement in Psoriatic Arthritis., PMID:35055107

Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes., PMID:35052491

IgD promotes pannus formation by activating Wnt5A-Fzd5-CTHRC1-NF-κB signaling pathway in FLS of CIA rats and the regulation of IgD-Fc-Ig fusion protein., PMID:34688134

WNT/β-Catenin Signaling Promotes TGF-β-Mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production., PMID:34576234

Targeting the Receptor Tyrosine Kinase ROR1 by Small Molecules., PMID:34490515

FOSL2 promotes VEGF-independent angiogenesis by transcriptionnally activating Wnt5a in breast cancer-associated fibroblasts., PMID:33754039

IGLV3-21R110 identifies an aggressive biological subtype of chronic lymphocytic leukemia with intermediate epigenetics., PMID:33211804

Non-Canonical WNT5A Signaling Through RYK Contributes to Aggressive Phenotype of the Rheumatoid Fibroblast-Like Synoviocytes., PMID:33178184

Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression., PMID:33167868

Wnt5a expression and prognosis in stage II-III colon cancer., PMID:33045677

Beta-catenin non-canonical pathway: A potential target for inflammatory and hyperproliferative state via expression of transglutaminase 2 in psoriatic skin keratinocyte., PMID:32816372

WNT5A augments cell invasiveness by inducing CXCL8 in HER2-positive breast cancer cells., PMID:32736336

Clinical Management and Therapeutic Strategies for the Thyroid-Associated Ophthalmopathy: Current and Future Perspectives., PMID:32567373

Non-canonical WNT5a regulates Epithelial-to-Mesenchymal Transition in the mouse ovarian surface epithelium., PMID:32546756

Fam70A binds Wnt5a to regulate meiosis and quality of mouse oocytes., PMID:32391621

Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammation., PMID:32317758

Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression., PMID:32228612

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-WNT5A Polyclonal Antibody [PME30701]
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