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Recombinant Human NOS3 Protein, N-His

Catalog #:   YHD84202 Specific References (106) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: P29474
Protein length: Arg756-Gly1161
Overview

Catalog No.

YHD84202

Expression system

E. coli

Species

Homo sapiens (Human)

Protein length

Arg756-Gly1161

Predicted molecular weight

47.50 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P29474

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Constitutive NOS, NOSIII, eNOS, Endothelial NOS, EC-NOS, Nitric oxide synthase, endothelial, NOS type III, NOS3, cNOS

Data Image
  • SDS-PAGE
    SDS PAGE for Recombinant Human NOS3 protein
References

Endothelial nitric oxide synthase-derived nitric oxide in the regulation of metabolism. PMID: 33042519

Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation. PMID: 34499618

Endothelial cation channel PIEZO1 controls blood pressure by mediating flow-induced ATP release. PMID: 27797339

Type I interferon activation and endothelial dysfunction in caveolin-1 insufficiency-associated pulmonary arterial hypertension. PMID: 33836561

MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. PMID: 35182051

NOS3 is involved in the increased protein and arginine metabolic response in muscle during early endotoxemia in mice. PMID: 15644457

How widespread is stable protein S-nitrosylation as an end-effector of protein regulation? PMID: 28189849

Virtual screening of active compounds from Artemisia argyi and potential targets against gastric ulcer based on Network pharmacology. PMID: 31005783

The deleterious impact of a non-synonymous SNP on protein structure and function is apparent in hypertension. PMID: 34961886

Resolution for sepsis? PMID: 15630034

Caveolae and signaling. PMID: 11353331

In utero hypoxia attenuated acetylcholine-mediated vasodilatation via CHRM3/p-NOS3 in fetal sheep MCA: role of ROS/ERK1/2. PMID: 35585170

Homocysteine reduces protein S-nitrosylation in endothelium. PMID: 25189662

Effects of polymorphisms of NOS3 and GNB3 genes on skin microvascular reactivity in normal pregnancy. PMID: 23667878

Activation of the STAT1 Pathway Accelerates Periodontitis in Nos3-/- Mice. PMID: 31329047

AMP-activated protein kinase is a key regulator of acute neurovascular permeability. PMID: 33712448

Involvement of NOS3 in RA-Induced neural differentiation of human NT2/D1 cells. PMID: 22987726

[Progress in genetic research on essential tremor]. PMID: 28981951

Nitric oxide synthase enzymes in the airways of mice exposed to ovalbumin: NOS2 expression is NOS3 dependent. PMID: 20953358

17beta-Estradiol induces protein S-nitrosylation in the endothelium. PMID: 19914929

How does Viagra protect the ischemic heart? PMID: 19346460

Mechanisms of NO neurotoxicity. PMID: 9932445

Expression and distribution of NOS1 and NOS3 in the myocardium of angiotensin II-infused rats. PMID: 11408389

Focus on: "G protein-dependent activation of smooth muscle eNOS via natriuretic peptide clearance receptor". PMID: 9843698

Novel protein interactions with endoglin and activin receptor-like kinase 1: potential role in vascular networks. PMID: 24319055

SIRT6 regulates endothelium-dependent relaxation by modulating nitric oxide synthase 3 (NOS3). PMID: 36720357

Candidate Oncogenes, ARHGAP4, NOS3, and OR51B5, for the Development of Scirrhous-type Gastric Cancer. PMID: 36288877

Global protein expression profiling underlines reciprocal regulation of caveolin 1 and endothelial nitric oxide synthase expression in ovariectomized sheep uterine artery by estrogen/progesterone replacement therapy. PMID: 16436525

It's a knockout! PMID: 9613207

Cellular signaling and NO production. PMID: 20082095

Blood pressure control goes nuclear. PMID: 15514165

Dietary protein source contributes to the risk of developing maternal syndrome in the Dahl salt-sensitive rat. PMID: 33971615

Inflammation, oxidative stress, and atherosclerosis. PMID: 15017368

Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. PMID: 21421555

Targeting Rho in cardiovascular disease. PMID: 11009552

Nitrosative stress and transcription. PMID: 14669980

S100A1: A novel and essential molecular component for postischemic angiogenesis. PMID: 23287450

Beta-actin: a regulator of NOS-3. PMID: 17878410

Transcriptional regulation of endothelial nitric oxide synthase expression in uterine artery endothelial cells by c-Jun/AP-1. PMID: 17933457

Phosphodiesterase 5 restricts NOS3/Soluble guanylate cyclase signaling to L-type Ca2+ current in cardiac myocytes. PMID: 19345227

Endothelium function biomarkers and carotid intima-media thickness changes in relation to NOS3 (rs2070744) and GNB3 (rs5443) genes polymorphism in the essential arterial hypertension. PMID: 35489051

Pattern of NOS2 and NOS3 mRNA expression in human A549 cells and primary cultured AEC II. PMID: 11880293

Interaction between endothelial nitric oxide synthase rs1799983, cholesteryl ester-transfer protein rs708272 and angiopoietin-like protein 8 rs2278426 gene variants highly elevates the risk of type 2 diabetes mellitus and cardiovascular disease. PMID: 29973202

Tyrosine phosphorylation of NOS3 in a breast cancer cell line and Src-transformed cells. PMID: 15069547

Effect of miR-134 against myocardial hypoxia/reoxygenation injury by directly targeting NOS3 and regulating PI3K/Akt pathway. PMID: 31618402

NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels. PMID: 29891729

Double tribble: two TRIB3 variants, insulin, Akt, and eNOS. PMID: 18565845

Signal transduction of eNOS activation. PMID: 10690325

Ischaemic preconditioning preferentially increases protein S-nitrosylation in subsarcolemmal mitochondria. PMID: 25694588

Expression of a NOS-III-like protein in human astroglial cell culture. PMID: 9837744

Thioredoxin-related regulation of NO/NOS activities. PMID: 12076971

Endothelial nitric oxide synthase in the amphibian, Xenopus tropicalis. PMID: 21199680

cGMP catabolism by phosphodiesterase 5A regulates cardiac adrenergic stimulation by NOS3-dependent mechanism. PMID: 15576651

[Association of AGT, ACE, NOS3, TNF, MMP9, CYBA polymorphism with subclinical arterial wall changes]. PMID: 33849420

Nontranscriptional actions of the glucocorticoid receptor. PMID: 12682725

The role of AMP-activated protein kinase in the expression of the dystrophin-associated protein complex in skeletal muscle. PMID: 29401588

Caveolae and caveolins in the cardiovascular system. PMID: 15192036

Molecular mechanisms underlying the activation of eNOS. PMID: 20012875

eNOS inhibition of proliferation: a role for p21(Sdi1/Cip1/Waf1) and p27(kip1). PMID: 10974212

Gene-Gene Interactions Among PRKCA, NOS3 and BDKRB2 Polymorphisms Affect the Antihypertensive Effects of Enalapril. PMID: 27696692

Vascular senescence and ageing: a role for the MEOX proteins in promoting endothelial dysfunction. PMID: 28727928

Genetic determinants of coronary vasomotor tone in humans. PMID: 11557533

Pharmacogenomics for the treatment of dementia. PMID: 12452480

Thioredoxin-interacting protein mediates sustained VEGFR2 signaling in endothelial cells required for angiogenesis. PMID: 23393387

Contributions of Nitric Oxide to AHR-Ligand-Mediated Keratinocyte Differentiation. PMID: 32784365

Functional study of 14-3-3 protein epsilon (YWHAE) in keratinocytes: microarray integrating bioinformatics approaches. PMID: 31244373

Proliferation of regulatory mechanisms for eNOS: an emerging role for the cytoskeleton. PMID: 12003771

Regulatory mechanisms of hepatic microcirculation. PMID: 14724338

Nitroglycerin tolerance in caveolin-1 deficient mice. PMID: 25158065

G-protein-coupled receptor kinase interactor-1 (GIT1) is a new endothelial nitric-oxide synthase (eNOS) interactor with functional effects on vascular homeostasis. PMID: 22294688

Activated protein C induction of MCP-1 in human endothelial cells: a possible role for endothelial cell nitric oxide synthase. PMID: 11672583

Identification of gene variants in NOS3, ET-1 and RAS that confer risk and protection against microangiopathy in type 2 diabetic obese subjects. PMID: 21406182

Suppression of protein phosphatase 2 differentially modulates VEGF- and FGF2-induced signaling in ovine fetoplacental artery endothelial cells. PMID: 19692121

Protein tyrosine nitration in the ventilatory muscles: role of nitric oxide synthases. PMID: 11919080

Purification of caveolae-derived membrane microdomains containing lipid-anchored signaling molecules, such as GPI-anchored proteins, H-Ras, Src-family tyrosine kinases, eNOS, and G-protein alpha-, beta-, and gamma-subunits. PMID: 10399145

MCP-induced protein 1 suppresses TNFalpha-induced VCAM-1 expression in human endothelial cells. PMID: 20561987

Exercise and cardiovascular health: get active to "AKTivate" your endothelial nitric oxide synthase. PMID: 12835404

Muscarinic-dependent phosphorylation of the cardiac ryanodine receptor by protein kinase G is mediated by PI3K-AKT-nNOS signaling. PMID: 32580946

Sepsis is associated with reciprocal expressional modifications of constitutive nitric oxide synthase (NOS) in human skeletal muscle: down-regulation of NOS1 and up-regulation of NOS3. PMID: 11546971

Nine genetic polymorphisms associated with power athlete status - A Meta-Analysis. PMID: 28666769

Increased renal oxidative stress in salt-sensitive human GRK4γ486V transgenic mice. PMID: 28189851

alpha1G-dependent T-type Ca2+ current antagonizes cardiac hypertrophy through a NOS3-dependent mechanism in mice. PMID: 19920353

Changes in histological structure and nitric oxide synthase expression in aorta of rats supplemented with bee pollen or whey protein. PMID: 30835492

Nitric oxide and Bartter and Gitelman syndromes. PMID: 11532116

Anthracycline-induced cardiotoxicity in women without cardiovascular diseases: molecular and genetic predictors. PMID: 34783301

Nongenomic, ER-mediated activation of endothelial nitric oxide synthase: how does it work? What does it mean? PMID: 11090536

Altered subcellular localization of heat shock protein 90 is associated with impaired expression of the aryl hydrocarbon receptor pathway in dogs. PMID: 23472125

Modern angiogenic thermal therapy from the country of traditional thermal therapy. PMID: 22664487

Nitric oxide synthase-3 promotes embryonic development of atrioventricular valves. PMID: 24204893

Do we kNOw how HSP90 and eNOS mediate lung injury in sickle cell disease? PMID: 15003934

Evidence for elevated (LIMK2 and CFL1) and suppressed (ICAM1, EZR, MAP2K2, and NOS3) gene expressions in metabolic syndrome. PMID: 26956845

Selective dysregulation of nitric oxide synthase type 3 in cardiac myocytes but not coronary microvascular endothelial cells of spontaneously hypertensive rat. PMID: 9747440

Characterization of the sex-dependent myocardial S-nitrosothiol proteome. PMID: 26702143

Angiogenesis related genes NOS3, CD14, MMP3 and IL4R are associated to VEGF gene expression and circulating levels in healthy adults. PMID: 26437765

Role of Akt signaling in vascular homeostasis and angiogenesis. PMID: 12089061

Akt signaling and its role in postnatal neovascularization. PMID: 15578445

An endocardial pathway involving Tbx5, Gata4, and Nos3 required for atrial septum formation. PMID: 20974940

Lipopolysaccharide down-regulates Sp1 binding activity by promoting Sp1 protein dephosphorylation and degradation. PMID: 12089157

Tumor necrosis factor alpha regulates nitric oxide synthase expression in portal hypertensive gastric mucosa of rats. PMID: 9537427

Endothelial nitric oxide synthase expression and its functionality. PMID: 10453308

Emerging mechanisms for secondary cardioprotective effects of statins. PMID: 11557228

Metabolic Reprogramming of Trophoblast Cells in Response to Hypoxia. PMID: 30627907

Akt1 mediates purinergic-dependent NOS3 activation in thick ascending limbs. PMID: 19570880

Nitric oxide synthase 1 and nitric oxide synthase 3 protein expression is regionally and temporally regulated in fetal brain. PMID: 8873971

Evaluation of placental protein modifications in normotensive and spontaneously hypertensive rats. PMID: 18358529

All in the family: VEGF-B joins the ranks of proangiogenic cytokines. PMID: 12881472

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant Human NOS3 Protein, N-His [YHD84202]
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