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Anti-GJA5 Polyclonal Antibody

Catalog #:   PHE17201 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P36382
Overview

Catalog No.

PHE17201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GJA5 (Tyr227-Val358).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Cx40,Gap junction alpha-5 protein,Connexin-40,GJA5

Purification

Purified by antigen affinity column.

Accession

P36382

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with GJA5 antibody (PHE17201) at 1 μg/ml.

    Lane 1: JEG-3

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 40 kDa
    Observed MW: 40 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat heart stained for GJA5 with PHE17201.
References

Large-Scale Single-Cell RNA-Seq Reveals Molecular Signatures of Heterogeneous Populations of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells., PMID:29986945

Mechanical stretch via transforming growth factor-β1 activates microRNA-208a to regulate hypertrophy in cultured rat cardiac myocytes., PMID:24120154

Role of tumor necrosis factor-α in the pathogenesis of atrial fibrosis and development of an arrhythmogenic substrate., PMID:23370453

Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis., PMID:22945766

Remodeling of the peripheral cardiac conduction system in response to pressure overload., PMID:22307665

Influence of v5/6-His tag on the properties of gap junction channels composed of connexin43, connexin40 or connexin45., PMID:21424225

Role of the gap junctions in the contractile response to agonists in pulmonary artery from two rat models of pulmonary hypertension., PMID:21414209

Betamethasone, progesterone and RU-486 (mifepristone) exert similar effects on connexin expression in trophoblast-derived HTR-8/SVneo cells., PMID:21211465

Mesenteric vascular remodeling in hyperhomocysteinemia., PMID:21076854

Connexin 43 remodeling induced by LMNA gene mutation Glu82Lys in familial dilated cardiomyopathy with atrial ventricular block., PMID:20497714

Histological substrate of human atrial fibrillation., PMID:20006465

Effect of high-frequency electromagnetic fields on trophoblastic connexins., PMID:19490996

Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37., PMID:19429814

The influence of carvedilol on atrial connexin 40 after myocardial infarction., PMID:18664019

Pulmonary venous structural remodeling in a canine model of chronic atrial dilation due to mitral regurgitation., PMID:18401472

Human atrial conduction and arrhythmogenesis correlates with conformational exposure of specific epitopes on the connexin40 carboxyl tail., PMID:16600287

Differential connexin expression in preglomerular and postglomerular vasculature: accentuation during diabetes., PMID:16105048

Alterations in connexin expression in the bladder of patients with urge symptoms., PMID:16104929

Rapid endothelial cell-selective loading of connexin 40 antibody blocks endothelium-derived hyperpolarizing factor dilation in rat small mesenteric arteries., PMID:16037574

Transcriptional regulation of the murine Connexin40 promoter by cardiac factors Nkx2-5, GATA4 and Tbx5., PMID:15537493

Chronic regulation of the expression of gap junction proteins connexin40, connexin43, and connexin45 in neonatal rat cardiomyocytes., PMID:15496289

Proteins of interstitial cells of Cajal and intestinal smooth muscle, colocalized with caveolin-1., PMID:15472013

The relationship between gap junctional remodeling and human atrial fibrillation., PMID:15361305

Gap junctions are required for trophoblast proliferation in early human placental development., PMID:15193866

Angiotensin II receptor blockade corrects altered expression of gap junctions in vascular endothelial cells from hypertensive rats., PMID:15016632

High glucose alters connexin 43 expression and gap junction intercellular communication activity in retinal pericytes., PMID:14638741

Gap junction-microtubule associations in rat alveolar epithelial cells., PMID:14604851

Connexin 40 expression in bovine and rat retinas., PMID:12662966

Comparison of connexin expression patterns in the developing mouse heart and human foetal heart., PMID:12619874

Connexin37 is the major connexin expressed in the media of caudal artery., PMID:12231561

Tissue structure and connexin expression of canine pulmonary veins., PMID:12176122

Heterogeneity in the distribution of vascular gap junctions and connexins: implications for function., PMID:12060107

Expression and regulation of connexins in cultured ventricular myocytes isolated from adult rat hearts., PMID:11889564

Heterogeneous expression of connexins in rabbit sinoatrial node cells: correlation between connexin isotype and cell size., PMID:11744016

Gap junctions in gastrointestinal muscle contain multiple connexins., PMID:11447034

Connexins 40 and 43 are differentially regulated within the kidneys of rats with renovascular hypertension., PMID:11422751

The gap-junctional protein connexin40 is elevated in patients susceptible to postoperative atrial fibrillation., PMID:11171793

Age- and myopathy-dependent changes in connexins of normal and cardiomyopathic Syrian hamster ventricular myocardium., PMID:10958169

Characterization of the rat connexin40 promoter: two Sp1/Sp3 binding sites contribute to transcriptional activation., PMID:10912461

Gap junctional remodeling in relation to stabilization of atrial fibrillation in the goat., PMID:10912458

Multiple connexin expression in regenerating arterial endothelial gap junctions., PMID:10894813

Intercellular communication in the immune system: differential expression of connexin40 and 43, and perturbation of gap junction channel functions in peripheral blood and tonsil human lymphocyte subpopulations., PMID:10792506

Heterogeneous localization of connexin40 in the renal vasculature., PMID:10625581

Expression of major gap junction connexin types in the working myocardium of eight chordates., PMID:10452821

Connexin45, a major connexin of the rabbit sinoatrial node, is co-expressed with connexin43 in a restricted zone at the nodal-crista terminalis border., PMID:10375379

Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells., PMID:10339616

Connexin45 (alpha 6) expression delineates an extended conduction system in the embryonic and mature rodent heart., PMID:10079513

Individual gap junction plaques contain multiple connexins in arterial endothelium., PMID:9851942

Conditional lineage ablation to model human diseases., PMID:9736743

Specific localization of gap junction protein, connexin45, in the deep muscular plexus of dog and rat small intestine., PMID:9582405

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-GJA5 Polyclonal Antibody [PHE17201]
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