Catalog No.
YHJ51905
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Arg309-Asp1025
Predicted molecular weight
96.01 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9BYX4
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
MDA-5, IFIH1, Helicard, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Interferon-induced with helicase C domain protein 1, Helicase with 2 CARD domains, RNA helicase-DEAD box protein 116, RIG-I-like receptor 2, Interferon-induced helicase C domain-containing protein 1, MDA5, Murabutide down-regulated protein, Melanoma differentiation-associated protein 5, CADM-140 autoantigen, RH116, RLR-2
Interstitial Lung Disease in Anti-MDA5 Positive Dermatomyositis. PMID: 33405101
Dermatomyositis With Anti-MDA5 Antibodies: Bioclinical Features, Pathogenesis and Emerging Therapies. PMID: 34745149
Anti-MDA5-associated dermatomyositis. PMID: 32270621
Anti-MDA5 dermatomyositis after COVID-19 vaccination: a case-based review. PMID: 35661906
Anti-MDA5 dermatomyositis: an update from bench to bedside. PMID: 36094462
Single-cell profiling reveals distinct adaptive immune hallmarks in MDA5+ dermatomyositis with therapeutic implications. PMID: 36309526
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. PMID: 16625202
Anti-melanoma differentiation-associated gene 5 (MDA5) dermatomyositis: A concise review with an emphasis on distinctive clinical features. PMID: 29229575
The intersection molecule MDA5 in Cancer and COVID-19. PMID: 36119095
Editorial: Anti-MDA5-Positive Dermatomyositis. PMID: 35712109
MDA5-filament, dynamics and disease. PMID: 25676875
Roles of biomarkers in anti-MDA5-positive dermatomyositis, associated interstitial lung disease, and rapidly progressive interstitial lung disease. PMID: 36221983
Comparative Structure and Function Analysis of the RIG-I-Like Receptors: RIG-I and MDA5. PMID: 31379819
Dermatomyositis: Clinical features and pathogenesis. PMID: 31279808
Anti-MDA5 Antibody Spectrum in Western World. PMID: 30382445
Breaching Self-Tolerance to Alu Duplex RNA Underlies MDA5-Mediated Inflammation. PMID: 29395326
MDA5 autoantibody-another indicator of clinical diversity in dermatomyositis. PMID: 28480196
Different Multivariable Risk Factors for Rapid Progressive Interstitial Lung Disease in Anti-MDA5 Positive Dermatomyositis and Anti-Synthetase Syndrome. PMID: 35320936
A Balancing Act: MDA5 in Antiviral Immunity and Autoinflammation. PMID: 30201512
A New Predictive Model for the Prognosis of MDA5+ DM-ILD. PMID: 35783655
[Dermatomyositis with anti-MDA5 antibodies]. PMID: 31626458
MDA5-positive dermatomyositis: an uncommon entity in Europe with variable clinical presentations. PMID: 26557046
Presence of Anti-MDA5 Antibody and Its Value for the Clinical Assessment in Patients With COVID-19: A Retrospective Cohort Study. PMID: 34987516
To "Z" or not to "Z": Z-RNA, self-recognition, and the MDA5 helicase. PMID: 33983939
MDA5 attenuate autophagy in chicken embryo fibroblasts infected with IBDV. PMID: 34406094
Prognostic values of anti-Ro52 antibodies in anti-MDA5-positive clinically amyopathic dermatomyositis associated with interstitial lung disease. PMID: 33331866
MDA5 expression is associated with TGF-β-induced fibrosis: potential mechanism of interstitial lung disease in anti-MDA5 dermatomyositis. PMID: 35412608
Monoclonal antibodies from B cells of patients with anti-MDA5 antibody-positive dermatomyositis directly stimulate interferon gamma production. PMID: 35436746
Anti-MDA5 Amyopathic Dermatomyositis-A Diagnostic and Therapeutic Challenge. PMID: 35892910
Cardiac involvement in anti-MDA5 dermatomyositis: a case-based review. PMID: 36454342
Innate Viral Sensor MDA5 and Coxsackievirus Interplay in Type 1 Diabetes Development. PMID: 32635205
Repeated MDA5 Gene Loss in Birds: An Evolutionary Perspective. PMID: 34834938
LGP2 synergy with MDA5 in RLR-mediated RNA recognition and antiviral signaling. PMID: 25794939
Pattern Recognition by Melanoma Differentiation-Associated Gene 5 (Mda5) in Teleost Fish: A Review. PMID: 31080451
Melanoma differentiation-Associated gene 5 protects against NASH in mice. PMID: 34482560
MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA. PMID: 34795277
Recurrence in long-term survivor of anti-MDA5 antibody-positive clinically amyopathic dermatomyositis: case series and literature review. PMID: 33560195
Clinical Features of Dermatomyositis/Polymyositis with Anti-MDA5 Antibody Positivity. PMID: 35992549
RNA sensor MDA5 suppresses LINE-1 retrotransposition by regulating the promoter activity of LINE-1 5'-UTR. PMID: 35414110
MDA5-dependent responses contribute to autoimmune diabetes progression and hindrance. PMID: 36512407
IFIH1 Contributes to M1 Macrophage Polarization in ARDS. PMID: 33519803
Genetic and phenotypic spectrum associated with IFIH1 gain-of-function. PMID: 31898846
IFIH1 loss-of-function variants contribute to very early-onset inflammatory bowel disease. PMID: 34185153
[Dermatomyositis: new antibody, new classification]. PMID: 31859626
Tofacitinib in Amyopathic Dermatomyositis-Associated Interstitial Lung Disease. PMID: 31314977
Effects of type 1 diabetes-associated IFIH1 polymorphisms on MDA5 function and expression. PMID: 26385483
Immunogenicity of In Vitro-Transcribed RNA. PMID: 34677064
Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. PMID: 29395325
The Significance of Autoantibodies in Juvenile Dermatomyositis. PMID: 34840975
Biomarkers of disease activity in dermatomyositis. PMID: 36082751
Association of IFIH1 rs1990760 polymorphism with susceptibility to autoimmune diseases: a meta-analysis. PMID: 23734776
Association Among MIF, IFIH1, and IL6 Gene Polymorphisms and Non-Segmental Vitiligo in a Chinese Han Population. PMID: 35983127
Interferon induced with helicase C domain 1 (IFIH1) and virus-induced autoimmunity: a review. PMID: 20121398
Hereditary spastic paraplegia associated with a rare IFIH1 mutation: a case report and literature review. PMID: 31427910
Polymorphisms in IFIH1: the good and the bad. PMID: 28632717
Polymorphisms in ACE1, TMPRSS2, IFIH1, IFNAR2, and TYK2 Genes Are Associated with Worse Clinical Outcomes in COVID-19. PMID: 36672770
Neurologic Phenotypes Associated with Mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR1, and IFIH1: Aicardi-Goutières Syndrome and Beyond. PMID: 27643693
Effects of IFIH1 rs1990760 variants on systemic inflammation and outcome in critically ill COVID-19 patients in an observational translational study. PMID: 35060899
The role of interferon induced with helicase C domain 1 (IFIH1) in the development of type 1 diabetes mellitus. PMID: 24402011
ADAR1 interaction with Z-RNA promotes editing of endogenous double-stranded RNA and prevents MDA5-dependent immune activation. PMID: 34380029
IFIH1/IRF1/STAT1 promotes sepsis associated inflammatory lung injury via activating macrophage M1 polarization. PMID: 36462334
ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity. PMID: 33727702
Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases. PMID: 33373584
Severe viral respiratory infections in children with IFIH1 loss-of-function mutations. PMID: 28716935
IFIH1 was predicted as a key biomarker in primary Sjögren's syndrome based on transcriptome analysis and experimental verification in patients and mice. PMID: 36973184
Reduced expression of IFIH1 is protective for type 1 diabetes. PMID: 20844740
Rituximab in the Treatment of Interstitial Lung Diseases Related to Anti-Melanoma Differentiation-Associated Gene 5 Dermatomyositis: A Systematic Review. PMID: 35116041
An origin of the immunogenicity of in vitro transcribed RNA. PMID: 29534222
Pangolins Lack IFIH1/MDA5, a Cytoplasmic RNA Sensor That Initiates Innate Immune Defense Upon Coronavirus Infection. PMID: 32574256
ADAR1 Prevents Autoinflammatory Processes in the Heart Mediated by IRF7. PMID: 36000401
Genetic Association Study of IL2RA, IFIH1, and CTLA-4 Polymorphisms With Autoimmune Thyroid Diseases and Type 1 Diabetes. PMID: 32974248
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility. PMID: 23535865
IRF1 Is Required for MDA5 (IFIH1) Induction by IFN-α, LPS, and poly(I:C) in Murine Macrophages. PMID: 36380629
A specific IFIH1 gain-of-function mutation causes Singleton-Merten syndrome. PMID: 25620204
For research use only. Not for human or drug use.
