Anti-IRS1 Polyclonal Antibody

Catalog No.

PHE12901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IRS1 (Pro4-Arg267).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

IRS-1,IRS1,Insulin receptor substrate 1

Purification

Purified by antigen affinity column.

Accession

P35568

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Facile Generation of Neutralizing Antibodies on Tyrosine Phosphorylated IRS1 by Epitope-Directed Elicitation., PMID:39137393

TGF-β phospho antibody array identifies altered SMAD2, PI3K/AKT/SMAD, and RAC signaling contribute to the pathogenesis of myxomatous mitral valve disease., PMID:37908840

PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain., PMID:37397533

Trastuzumab-resistant breast cancer cells-derived tumor xenograft models exhibit distinct sensitivity to lapatinib treatment in vivo., PMID:37370010

Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer., PMID:36774408

Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth., PMID:36551732

Novel 2,6,9-Trisubstituted Purines as Potent CDK Inhibitors Alleviating Trastuzumab-Resistance of HER2-Positive Breast Cancers., PMID:36145262

The Role of ARID1A in the Nonestrogenic Modulation of IGF-1 Signaling., PMID:35320351

A Novel Tree Shrew Model of Diabetic Retinopathy., PMID:35046899

Double Labeling Fluorescent Immunocytochemistry., PMID:34859404

Relationship between IGF-1 and body weight in inflammatory bowel diseases: Cellular and molecular mechanisms involved., PMID:34601192

Physiologic IGFBP7 levels prolong IGF1R activation in acute lymphoblastic leukemia., PMID:34438446

INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats., PMID:34345983

Lack of Brain Insulin Receptor Substrate-1 Causes Growth Retardation, With Decreased Expression of Growth Hormone-Releasing Hormone in the Hypothalamus., PMID:33980693

Disruption of FOXO3a-miRNA feedback inhibition of IGF2/IGF-1R/IRS1 signaling confers Herceptin resistance in HER2-positive breast cancer., PMID:33976188

PCR Array Technology in Biopsy Samples Identifies Up-Regulated mTOR Pathway Genes as Potential Rejection Biomarkers After Kidney Transplantation., PMID:33681239

Peripheral inflammatory biomarkers define biotypes of bipolar depression., PMID:33658605

Autoimmune activation of the GnRH receptor induces insulin resistance independent of obesity in a female rat model., PMID:33356018

GLP-1 preserves β cell function via improvement on islet insulin signaling in high fat diet feeding mice., PMID:33307381

Insulin receptor substrate 1 may play divergent roles in human colorectal cancer development and progression., PMID:32821075

Phosphorylated and O-GlcNAc Modified IRS-1 (Ser1101) and -2 (Ser1149) Contribute to Human Diabetes Type II., PMID:32798372

In vitro characterization of the effects of chronic insulin stimulation in mouse 3T3-L1 and human SGBS adipocytes., PMID:32718202

Silencing of NAMPT leads to up-regulation of insulin receptor substrate 1 gene expression in U87 glioma cells., PMID:32597148

Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells., PMID:32198214

Infliximab ameliorates tumor necrosis factor-alpha exacerbated renal insulin resistance induced in rats by regulating insulin signaling pathway., PMID:32004528

NK cells induce hepatic ER stress to promote insulin resistance in obesity through osteopontin production., PMID:31829469

CDK12 drives breast tumor initiation and trastuzumab resistance via WNT and IRS1-ErbB-PI3K signaling., PMID:31468695

Cullin7 enhances resistance to trastuzumab therapy in Her2 positive breast cancer via degrading IRS-1 and downregulating IGFBP-3 to activate the PI3K/AKT pathway., PMID:31461670

Hesperidin inhibits keratinocyte proliferation and imiquimod-induced psoriasis-like dermatitis via the IRS-1/ERK1/2 pathway., PMID:30658103

Utility of Neuronal-Derived Exosomes to Examine Molecular Mechanisms That Affect Motor Function in Patients With Parkinson Disease: A Secondary Analysis of the Exenatide-PD Trial., PMID:30640362

Fibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance models., PMID:30445954

Hyperinsulinemia precedes insulin resistance in offspring rats exposed to angiotensin II type 1 autoantibody in utero., PMID:30101377

Interleukin-4 Restores Insulin Sensitivity in Lipid-Induced Insulin-Resistant Adipocytes., PMID:29738684

Helicobacter pylori infection aggravates diet-induced nonalcoholic fatty liver in mice., PMID:29657020

Electroacupuncture mitigates endothelial dysfunction via effects on the PI3K/Akt signalling pathway in high fat diet-induced insulin-resistant rats., PMID:29502072

Liraglutide ameliorates palmitate-induced insulin resistance through inhibiting the IRS-1 serine phosphorylation in mouse skeletal muscle cells., PMID:29374854

Losartan counteracts the effects of cardiomyocyte swelling on glucose uptake and insulin receptor substrate-1 levels., PMID:28889965

Single nucleotide polymorphisms in the IGF-IRS pathway are associated with outcome in mCRC patients enrolled in the FIRE-3 trial., PMID:28369940

Snail-Modulated MicroRNA 493 Forms a Negative Feedback Loop with the Insulin-Like Growth Factor 1 Receptor Pathway and Blocks Tumorigenesis., PMID:27956702

IGF-1R and mTOR Blockade: Novel Resistance Mechanisms and Synergistic Drug Combinations for Ewing Sarcoma., PMID:27576731

Overexpressing IRS1 in Endothelial Cells Enhances Angioblast Differentiation and Wound Healing in Diabetes and Insulin Resistance., PMID:27217486

Prevention Effects and Possible Molecular Mechanism of Mulberry Leaf Extract and its Formulation on Rats with Insulin-Insensitivity., PMID:27054886

IRS2 silencing increases apoptosis and potentiates the effects of ruxolitinib in JAK2V617F-positive myeloproliferative neoplasms., PMID:26755644

MicroRNA-1225-5p inhibits proliferation and metastasis of gastric carcinoma through repressing insulin receptor substrate-1 and activation of β-catenin signaling., PMID:26684358

Inhibitory Effect of Memantine on Streptozotocin-Induced Insulin Receptor Dysfunction, Neuroinflammation, Amyloidogenesis, and Neurotrophic Factor Decline in Astrocytes., PMID:26660109

Nck influences preosteoblastic/osteoblastic migration and bone mass., PMID:26621720

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors., PMID:26297026

Cell lysis-free quantum dot multicolor cellular imaging-based mechanism study for TNF-α-induced insulin resistance., PMID:25623542

Rationale for co-targeting IGF-1R and ALK in ALK fusion-positive lung cancer., PMID:25173427

Etanercept restores normal insulin signal transduction in β2-adrenergic receptor knockout mice., PMID:25138272

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