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Anti-BCO1 Polyclonal Antibody

Catalog #:   PHK04701 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9HAY6
Overview

Catalog No.

PHK04701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BCO1 (Gly6-Pro206).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

BCMO1, Beta,beta-carotene 15,15'-dioxygenase, BCDO, BCO1, Beta-carotene oxygenase 1, BCDO1, Beta-carotene dioxygenase 1

Purification

Purified by antigen affinity column.

Accession

Q9HAY6

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with BCO1 antibody (PHK04701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with BCO1 antibody (PHK04701) at 1 μg/ml.

    Lane 1: Human Placenta

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 63 kDa
    Observed MW: 63 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human testis stained for BCO1 with PHK04701.
References

Unveiling BCO2 function in macular pigment metabolism: Mitochondrial processing and expression in the primate retina., PMID:39978586

β-Carotene accelerates the resolution of atherosclerosis in mice., PMID:38319073

β-carotene accelerates the resolution of atherosclerosis in mice., PMID:36945561

ISX is a retinoic acid-sensitive gatekeeper that controls intestinal beta,beta-carotene absorption and vitamin A production., PMID:20061533

Cell type-specific expression of beta-carotene 9',10'-monooxygenase in human tissues., PMID:15983114

Cell type-specific expression of beta-carotene 15,15'-mono-oxygenase in human tissues., PMID:15034000

Expression of beta-carotene 15,15' monooxygenase in retina and RPE-choroid., PMID:12506054

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-BCO1 Polyclonal Antibody [PHK04701]
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