Overview
Catalog No.
PHE05701
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human HSPA1L (Met1-Asp641).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
HSP70-Hom,Heat shock 70 kDa protein 1L,Heat shock 70 kDa protein 1-like,HSPA1L,Heat shock 70 kDa protein 1-Hom
Purification
Purified by antigen affinity column.
Accession
P34931
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Various lysates were subjected to SDS PAGE followed by western blot with HSPA1L antibody (PHE05701) at 1μg/ml.
Lane 1: PC12 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Human plasma lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse liver lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse lung lysate
Lane 8: Rat spleen lysate
Lane 9: Rat liver lysate
Lane 10: Rat kidney lysate
Lane 11: Rat lung lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 70 kDa
Observed MW: 70 kDa
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Immunohistochemical analysis of rat brain stained for HSPA1L with PHE05701.
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA1L antibody (PHE05701) at 1 µg/ml.
Lane 1: Recombinant Protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 73 kDa
Observed MW: 73 kDa
References
Expressions of HSPA1L and HSPA9 are associated with poor sperm quality of low-motility spermatozoa in fertile men., PMID:34796524
The Cellular Prion Protein: A Promising Therapeutic Target for Cancer., PMID:33276687
Antibody-validated proteins in inflamed islets of fulminant type 1 diabetes profiled by laser-capture microdissection followed by mass spectrometry., PMID:25329145
Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome., PMID:21849784
Heat shock proteins on the human sperm surface., PMID:19962198
Association analysis of heat shock protein 70 gene polymorphisms in schizophrenia., PMID:18299791
Datasheet
For research use only. Not for human or drug use.
