Catalog No.
PHD88401
Species reactivity
Human, Mouse
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CDC25B (Cys377-Gly566).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Dual specificity phosphatase Cdc25B,CDC25B,CDC25HU2,M-phase inducer phosphatase 2
Purification
Purified by antigen affinity column.
Accession
P30305
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nasal polyp antibody-secreting cells display proliferation signature in aspirin-exacerbated respiratory disease., PMID:37898408
Isosteviol derivatives as protein tyrosine Phosphatase-1B inhibitors: Synthesis, biological evaluation and molecular docking., PMID:36963270
Cordyceps militaris extract induces apoptosis and pyroptosis via caspase-3/PARP/GSDME pathways in A549 cell line., PMID:35035907
Multi-Epitope-Based Vaccines for Colon Cancer Treatment and Prevention., PMID:34526999
Three-dimensional CRISPR screening reveals epigenetic interaction with anti-angiogenic therapy., PMID:34267311
The Suppressing Effects of Dkk3 Expression on Aggressiveness and Tumorigenesis of Colorectal Cancer., PMID:33425757
Protein kinase Cδ (PKCδ) involved in the regulation of pAkt1 (Ser473) on the release of mouse oocytes from diplotene arrest., PMID:29774951
mRNA profiling identifies low levels of phosphatases dual‐specific phosphatase‐7 (DUSP7) and cell division cycle‐25B (CDC25B) in patients with early arthritis., PMID:28253537
Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397., PMID:27889774
CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos., PMID:27885657
Human Cytomegalovirus miR-UL148D Facilitates Latent Viral Infection by Targeting Host Cell Immediate Early Response Gene 5., PMID:27824944
Differential Protein Expression in Small Intestinal Neuroendocrine Tumors and Liver Metastases., PMID:26474427
Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells., PMID:26108159
Overexpression of mutant cell division cycle 25 homolog B (CDC25B) enhances the efficiency of selection in Chinese hamster ovary cells., PMID:24248275
Cdc25B phosphatase participates in maintaining metaphase II arrest in mouse oocytes., PMID:23661366
Identification of N-terminally truncated stable nuclear isoforms of CDC25B that are specifically involved in G2/M checkpoint recovery., PMID:21363925
A novel N-hydroxy-N'-aminoguanidine derivative inhibits ribonucleotide reductase activity: Effects in human HL-60 promyelocytic leukemia cells and synergism with arabinofuranosylcytosine (Ara-C)., PMID:20849828
The FOXM1 transcriptional factor promotes the proliferation of leukemia cells through modulation of cell cycle progression in acute myeloid leukemia., PMID:20823107
Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma., PMID:20813067
Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes., PMID:20500813
The polo-like kinase 1 regulates CDC25B-dependent mitosis entry., PMID:19185590
Protein kinase A modulates Cdc25B activity during meiotic resumption of mouse oocytes., PMID:19035343
Proteomics-based identification of autoantibody against CDC25B as a novel serum marker in esophageal squamous cell carcinoma., PMID:18722351
CDC25B acts as a potential target of PRKACA in fertilized mouse eggs., PMID:18633139
[Prognostic value of CDC25 phosphatases expression in laryngeal cancer]., PMID:18551998
Forkhead transcription factor FoxM1 regulates mitotic entry and prevents spindle defects in cerebellar granule neuron precursors., PMID:17893320
Expression of various CDC25B isoforms in human spermatozoa., PMID:17336969
CDC25B phosphorylation by p38 and MK-2., PMID:16861915
Genotoxic-activated G2-M checkpoint exit is dependent on CDC25B phosphatase expression., PMID:16818502
Cytoplasmic polyadenylation controls cdc25B mRNA translation in rat oocytes resuming meiosis., PMID:16816330
Cell-cycle-dependent cortical localization of pEg3 protein kinase in Xenopus and human cells., PMID:16159311
CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis., PMID:15908796
Characterisation of Cdc25B localisation and nuclear export during the cell cycle and in response to stress., PMID:15456846
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition., PMID:15128871
Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas., PMID:14559803
14-3-3 acts as an intramolecular bridge to regulate cdc25B localization and activity., PMID:12764136
[Tumor markers for colorectal cancer]., PMID:11579645
Constitutive Cdc25B tyrosine phosphatase activity in adult brain neurons with M phase-type alterations in Alzheimer's disease., PMID:11516829
Activation of Src in human breast tumor cell lines: elevated levels of phosphotyrosine phosphatase activity that preferentially recognizes the Src carboxy terminal negative regulatory tyrosine 530., PMID:10022129
The cdc25B phosphatase is essential for the G2/M phase transition in human cells., PMID:9683638
Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells., PMID:8743955
Cdc25A is a novel phosphatase functioning early in the cell cycle., PMID:8156993
Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins., PMID:1836978
For research use only. Not for human or drug use.
