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Recombinant ASFV p54/pE183L Protein, C-His

Catalog #:   EVV16001 Specific References (121) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: Insect Cells
Accession: Q65194
Protein length: Met1-Leu183
Overview

Catalog No.

EVV16001

Expression system

Insect Cells

Species

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)

Protein length

Met1-Leu183

Predicted molecular weight

20.94 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

Q65194

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Inner membrane protein p54, pE183L, Ba71V-126, E183L

Data Image
References

Epitope mapping of African swine fever virus (ASFV) structural protein, p54. PMID: 32004574

HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies. PMID: 35612629

Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice. PMID: 34383171

An extensive evaluation of codon usage pattern and bias of structural proteins p30, p54 and, p72 of the African swine fever virus (ASFV). PMID: 34901328

Development of a novel real-time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam. PMID: 34388311

Roles of African Swine Fever Virus Structural Proteins in Viral Infection. PMID: 29978065

Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes. PMID: 35498728

African swine fever virus: A raised global upsurge and a continuous threaten to pig husbandry. PMID: 35526679

Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54. PMID: 33737177

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. PMID: 36366433

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay. PMID: 35698809

Computational analysis of immunogenic epitopes in the p30 and p54 proteins of African swine fever virus. PMID: 36148815

Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine. PMID: 32915442

Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool. PMID: 37191526

Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis. PMID: 33562314

The structural protein p54 is essential for African swine fever virus viability. PMID: 8725112

A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies. PMID: 37112846

Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. PMID: 34638027

ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice. PMID: 36965632

ASFV in Tanzania: asymptomatic pigs harbor virus of molecular similarity to Georgia 2007. PMID: 23398669

Characterization and molecular basis of heterogeneity of the African swine fever virus envelope protein p54. PMID: 7933107

The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis. PMID: 15225638

Indirect ELISA Using Multi-Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs. PMID: 36560663

Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks. PMID: 33454861

Molecular characterization of African Swine fever viruses in Burkina Faso, Mali, and Senegal 1989-2016: Genetic diversity of ASFV in West Africa. PMID: 34323385

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide. PMID: 34715492

Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes. PMID: 19009341

Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS). PMID: 34815032

Screening and identification of the dominant antigens of the African swine fever virus. PMID: 37215478

African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites. PMID: 15047843

Multiple variants of African swine fever virus circulating in Vietnam. PMID: 35190886

Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin. PMID: 21094642

Expression and Immunogenicity of Recombinant African Swine Fever Virus Proteins Using the Semliki Forest Virus. PMID: 35615248

Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions. PMID: 34799123

Symptomatic and asymptomatic cases of African swine fever in Tanzania. PMID: 31325213

Splenic-targeting biomimetic nanovaccine for elevating protective immunity against virus infection. PMID: 36463277

Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China. PMID: 35782548

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum. PMID: 34454001

African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein. PMID: 11559815

Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip. PMID: 32887174

Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera. PMID: 16490226

Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells. PMID: 36211218

Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus. PMID: 36038241

Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate. PMID: 36016353

Comparison of mucosal immune responses to African swine fever virus antigens intranasally delivered with two different viral vectors. PMID: 35849881

Genetic Characterization of African Swine Fever Virus in Various Outbreaks in Central and Southern Vietnam During 2019-2021. PMID: 36209177

Molecular detection and characterization of African swine fever virus from field outbreaks in domestic pigs, Mizoram, India. PMID: 34743424

Recombinant antigen targets for serodiagnosis of African swine fever. PMID: 19420186

Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection. PMID: 14980493

First detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congo. PMID: 32883295

DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain. PMID: 30696015

Detection and genetic characterization of African swine fever virus (ASFV) in clinically infected pigs in two districts in South Kivu province, Democratic Republic Congo. PMID: 33732937

BacMam immunization partially protects pigs against sublethal challenge with African swine fever virus. PMID: 23428670

Unpicking the Secrets of African Swine Fever Viral Replication Sites. PMID: 33429879

Genetic characterization of African swine fever virus in Cameroon, 2010-2018. PMID: 30929230

Molecular characterization of African swine fever viruses from Burkina Faso, 2018. PMID: 35151326

Orally administered recombinant Lactobacillus expressing African swine fever virus antigens that induced immunity responses. PMID: 36699597

Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013. PMID: 24996815

Design and construction of African swine fever virus chimeras incorporating foreign viral epitopes. PMID: 10481737

Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011-2013) reveals rapid B602L central variable region evolution. PMID: 29143187

Detection of African swine fever virus in the tissues of asymptomatic pigs in smallholder farming systems along the Kenya-Uganda border: implications for transmission in endemic areas and ASF surveillance in East Africa. PMID: 28721858

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus. PMID: 36189357

Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus. PMID: 30367292

Evidence for the presence of African swine fever virus in apparently healthy pigs in South-Kivu Province of the Democratic Republic of Congo. PMID: 31902515

Characterization of a Novel African Swine Fever Virus p72 Genotype II from Nigeria. PMID: 37112895

African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. PMID: 33725423

Diversity and emergence of new variants of African swine fever virus Genotype I circulating in domestic pigs in Nigeria (2016-2018). PMID: 36377750

Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens. PMID: 34399310

DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies. PMID: 23049728

Genetic Characterization of Circulating African Swine Fever Viruses in Nigeria (2007-2015). PMID: 27480888

Re-emergence of genotype I of African swine fever virus in Ivory Coast. PMID: 30548809

The Novel Antigenic Epitopes of African Swine Fever Virus Inner Membrane p54 Protein Revealed by Monoclonal Antibodies., PMID:40362108

Encapsulin nanoparticle-conjugated p54 protein boosts immune responses against African swine fever virus., PMID:40324494

Development and efficacy of a novel mRNA cocktail for the delivery of African swine fever virus antigens and induction of immune responses., PMID:40298440

Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs., PMID:40250107

Characterization of African swine fever virus in Angola, 2024., PMID:40131561

A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs., PMID:39852784

p54-Fc-Labeled Gold Nanoparticle-Based Lateral Flow Strip-Assisted Portable Devices for Rapid and Quantitative Point-of-Care Detection of ASFV Antibodies., PMID:39852075

Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever., PMID:39599534

A Nanobody-based TRIM-away targets the intracellular protein degradation of African swine fever virus., PMID:39488886

Establishment of an ELISA Based on a Recombinant Antigenic Protein Containing Multiple Prominent Epitopes for Detection of African Swine Fever Virus Antibodies., PMID:38792774

Preparation and epitope analysis of monoclonal antibodies against African swine fever virus DP96R protein., PMID:38734611

The proteomic analysis uncovers the cellular responses to the African swine fever virus membrane proteins p54, p17, and pB117L., PMID:38697277

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54., PMID:38203508

ASFV epitope mapping by high density peptides microarrays., PMID:38029799

Comparative Analysis of Swine Antibody Responses following Vaccination with Live-Attenuated and Killed African Swine Fever Virus Vaccines., PMID:38006019

A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs., PMID:38004769

A blocking ELISA based on virus-like nanoparticles chimerized with an antigenic epitope of ASFV P54 for detecting ASFV antibodies., PMID:37968284

Establishment and characterization of a novel indirect ELISA method based on ASFV antigenic epitope-associated recombinant protein., PMID:37865977

Genetic analysis of African swine fever viruses based on E183L (p54) gene, circulating in South Sumatra and Lampung province, Indonesia., PMID:37859961

Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens., PMID:37720232

A new vaccination regimen using adenovirus-vectored vaccine confers effective protection against African swine fever virus in swine., PMID:37401832

Evaluation of humoral and cellular immune responses induced by a cocktail of recombinant African swine fever virus antigens fused with OprI in domestic pigs., PMID:37237390

Screening and identification of the dominant antigens of the African swine fever virus., PMID:37215478

Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool., PMID:37191526

A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies., PMID:37112846

ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice., PMID:36965632

Dynamics of Serological and Mucosal Antibody Responses against African Swine Fever Viruses in Experimentally Infected Pigs., PMID:40303747

Orally administered recombinant Lactobacillus expressing African swine fever virus antigens that induced immunity responses., PMID:36699597

Indirect ELISA Using Multi-Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs., PMID:36560663

Diversity and emergence of new variants of African swine fever virus Genotype I circulating in domestic pigs in Nigeria (2016-2018)., PMID:36377750

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus., PMID:36366433

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus., PMID:36189357

Computational analysis of immunogenic epitopes in the p30 and p54 proteins of African swine fever virus., PMID:36148815

Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus., PMID:36038241

Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate., PMID:36016353

Comparison of mucosal immune responses to African swine fever virus antigens intranasally delivered with two different viral vectors., PMID:35849881

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay., PMID:35698809

Expression and Immunogenicity of Recombinant African Swine Fever Virus Proteins Using the Semliki Forest Virus., PMID:35615248

HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies., PMID:35612629

African swine fever virus: A raised global upsurge and a continuous threaten to pig husbandry., PMID:35526679

Molecular characterization of African swine fever viruses from Burkina Faso, 2018., PMID:35151326

Genetic Characterization and Variation of African Swine Fever Virus China/GD/2019 Strain in Domestic Pigs., PMID:35056045

An extensive evaluation of codon usage pattern and bias of structural proteins p30, p54 and, p72 of the African swine fever virus (ASFV)., PMID:34901328

Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS)., PMID:34815032

Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions., PMID:34799123

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide., PMID:34715492

Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA., PMID:34638027

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum., PMID:34454001

Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens., PMID:34399310

Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice., PMID:34383171

Datasheet
$ 430
Product specifications
100 μg 430 1 mg 2666

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Recombinant ASFV p54/pE183L Protein, C-His [EVV16001]
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