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Anti-SERPINB3 Polyclonal Antibody

Catalog #:   PHD84401 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P29508
Overview

Catalog No.

PHD84401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SERPINB3 (Met1-Ile210).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Serpin B3,SCCA,Protein T4-A,SERPINB3,SCCA1,Squamous cell carcinoma antigen 1,SCCA-1

Purification

Purified by antigen affinity column.

Accession

P29508

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SERPINB3 antibody (PHD84401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
  • Immunocytochemistry/ Immunofluorescence
    SERPINB3 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with SERPINB3 (PHD84401) at 10.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors for Non-Small Cell Lung Cancer Diagnosis., PMID:39771637

Ultrasensitive detection of SCCA employing a graphene oxide integrated microfiber ring laser biosensor., PMID:39276440

Impact of anti-squamous cell carcinoma antigen antibodies on serum squamous cell carcinoma antigen levels measured by chemiluminescent immunoassay and chemiluminescent enzyme immunoassay., PMID:37706549

Epitope-Specific Anti-SerpinB3 Antibodies for SerpinB3 Recognition and Biological Activity Inhibition., PMID:37238609

Photoelectrochemical immunoassay of squamous cell carcinoma antigen based on CuO/nitrogen-doped porous carbon-ZnO biolabeling and a type-II In2O3/AgBiS2 heterojunction., PMID:37099090

β-Cyclodextrin-functionalized Ti3C2Tx MXene nanohybrids as innovative signal amplifiers for the electrochemical sandwich-like immunosensing of squamous cell carcinoma antigen., PMID:36810629

Bile detection of squamous cell carcinoma antigen (SCCA) in extrahepatic cholangiocarcinoma., PMID:36369195

Ultrasensitive sandwich-typed electrochemical immunoassay for detection of squamous cell carcinoma antigen based on highly branched PtCo nanocrystals and dendritic mesoporous SiO2@AuPt nanoparticles., PMID:36219254

Genome-wide siRNA screening reveals several host receptors for the binding of human gut commensal Bifidobacterium bifidum., PMID:35768415

Combination of squamous cell carcinoma antigen immunocomplex and alpha-fetoprotein in mid- and long-term prediction of hepatocellular carcinoma among cirrhotic patients., PMID:35068873

Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study., PMID:34727105

Higher serum homocysteine level and frequency of hyperhomocysteinemia in carcinoembryonic antigen-positive or squamous cell carcinoma-antigen-positive oral leukoplakia patients., PMID:34175159

Prognostic Role for CYFRA 21-1 in Patients With Advanced-stage NSCLC Treated With Bevacizumab Plus Chemotherapy., PMID:33813413

SCCA-IgM in hepatocellular carcinoma patients treated with transarterial chemoembolization: gender-related differences., PMID:32808827

Squamous cell carcinoma antigen-IgM (SCCA-IgM) is associated with interstitial lung disease in systemic sclerosis., PMID:32087361

Divalent ion-induced aggregation of gold nanoparticles for voltammetry Immunosensing: comparison of transducer signals in an assay for the squamous cell carcinoma antigen., PMID:31938893

Serum Squamous Cell Carcinoma Antigen-Immunoglobulin M complex levels predict survival in patients with cirrhosis., PMID:31882893

Elevating performance of electrochemical immunosensor via photo-induced microscale hyperthermia in situ., PMID:31818758

Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor., PMID:31468573

Ionic liquid and spatially confined gold nanoparticles enhanced photoelectrochemical response of zinc-metal organic frameworks and immunosensing squamous cell carcinoma antigen., PMID:31376714

SCCA-IgM as a Potential Biomarker of Non-Alcoholic Fatty Liver Disease in Patients with Obesity, Prediabetes and Diabetes Undergoing Sleeve Gastrectomy., PMID:31104052

Squamous Cell Carcinoma Biomarker Sensing on a Strontium Oxide-Modified Interdigitated Electrode Surface for the Diagnosis of Cervical Cancer., PMID:31080815

Facile fabrication of visible light photoelectrochemical immunosensor for SCCA detection based on BiOBr/Bi2S3 heterostructures via self-sacrificial synthesis method., PMID:30876581

The effectiveness of serum midkine in detecting esophageal squamous cell carcinoma., PMID:30778774

SERPINB3 Delays Glomerulonephritis and Attenuates the Lupus-Like Disease in Lupus Murine Models by Inducing a More Tolerogenic Immune Phenotype., PMID:30254646

Serum Squamous Cell Carcinoma Antigen in Psoriasis: A Potential Quantitative Biomarker for Disease Severity., PMID:29870999

SCCA, TSGF, and the Long Non-Coding RNA AC007271.3 are Effective Biomarkers for Diagnosing Oral Squamous Cell Carcinoma., PMID:29763905

Development of a novel diagnostic algorithm to predict NASH in HCV-positive patients., PMID:29712495

Diagnostic accuracy of SCCA and SCCA-IgM for hepatocellular carcinoma: A meta-analysis., PMID:29704434

A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of carcinoembryonic antigen and squamous cell carcinoma antigen., PMID:29679420

Functional characterization of the clade B serine protease inhibitor SerpinB3 in the pacific white shrimp Litopenaeus vannamei., PMID:29550271

Clinical Value of Serum p53 Antibody in the Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma., PMID:29491120

Characterization of SCCA-IgM as a biomarker of liver disease in an Asian cohort of patients., PMID:29381084

Improved screen-printed carbon electrode for multiplexed label-free amperometric immuniosensor: Addressing its conductivity and reproducibility challenges., PMID:29107882

Circulating SCCA-IgM complex is a useful biomarker to predict the outcome of therapy in hepatocellular carcinoma patients., PMID:28609160

Squamous Cellular Carcinoma Antigen Serum Determination as a Biomarker of Barrett Esophagus and Esophageal Cancer: A Phase III Study., PMID:28422774

Potential-resolved "in-electrode" type electrochemiluminescence immunoassay based on functionalized g-C3N4 nanosheet and Ru-NH2 for simultaneous determination of dual targets., PMID:28412657

Ultrasensitive electrochemical immunosensor for quantitative detection of SCCA using Co3O4@CeO2-Au@Pt nanocomposite as enzyme-mimetic labels., PMID:28182976

Existence of a squamous cell carcinoma antigen-immunoglobulin complex causes a deviation between squamous cell carcinoma antigen concentrations determined using two different immunoassays: first report of squamous cell carcinoma antigen coupling with immunoglobulin A., PMID:28084094

Recurrent SERPINB3 and SERPINB4 mutations in patients who respond to anti-CTLA4 immunotherapy., PMID:27668655

Prognostic Significance of Cytokeratin 19 and Squamous Cell Cancer Antigen in Histologically Negative Sentinel Lymph Nodes of Cervical Squamous Cell Carcinoma., PMID:27654256

Diagnostic value of serum squamous cell carcinoma antigen for hepatocellular carcinoma: a systematic review and meta-analysis., PMID:27762143

An "in-electrode"-type immunosensing strategy for the detection of squamous cell carcinoma antigen based on electrochemiluminescent AuNPs/g-C3N4 nanocomposites., PMID:27591611

Periostin as a biomarker for the diagnosis of pediatric asthma., PMID:27062336

Ultrasensitive electrochemical immunosensor for SCCA detection based on ternary Pt/PdCu nanocube anchored on three-dimensional graphene framework for signal amplification., PMID:26700578

Marked decrease in serum squamous cell carcinoma antigen level after antitumor necrosis factor alpha therapy in six cases of severe psoriasis., PMID:26696364

Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer., PMID:26690745

Multifunctional reduced graphene oxide trigged chemiluminescence resonance energy transfer: Novel signal amplification strategy for photoelectrochemical immunoassay of squamous cell carcinoma antigen., PMID:26686924

Squamous cell carcinoma antigen-IgM is associated with hepatocellular carcinoma in patients with cirrhosis: A prospective study., PMID:26614642

[Preparation of recombinant serpins B3 and B4 and investigation of their specific interactions with antibodies using hydrogel-based microarrays]., PMID:26510597

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-SERPINB3 Polyclonal Antibody [PHD84401]
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