Catalog No.
EVV15101
Expression system
Mammalian Cells
Species
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Protein length
gH [Ser19-Leu682] & gL [Asn23-Gly137] & gp42 [Arg30-Ser223]
Predicted molecular weight
74 kDa & 14 kDa & 25 kDa (Reduced)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P03231 & P03212 & P03205
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Envelope glycoprotein H, gH, gH, BXLF2, Envelope glycoprotein L, gL, gL, BKRF2, Glycoprotein 42, gp42, Soluble gp42, BZLF2
Epstein Barr Virus: Development of Vaccines and Immune Cell Therapy for EBV-Associated Diseases. PMID: 34691042
A bivalent Epstein-Barr virus vaccine induces neutralizing antibodies that block infection and confer immunity in humanized mice. PMID: 35507671
Immunization with a self-assembling nanoparticle vaccine displaying EBV gH/gL protects humanized mice against lethal viral challenge. PMID: 35705092
gH/gL supercomplexes at early stages of herpesvirus entry. PMID: 26849495
Epstein-Barr virus gH/gL has multiple sites of vulnerability for virus neutralization and fusion inhibition. PMID: 36306784
Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. PMID: 29292383
Immunization with Components of the Viral Fusion Apparatus Elicits Antibodies That Neutralize Epstein-Barr Virus in B Cells and Epithelial Cells. PMID: 30979688
Stuck in the middle: structural insights into the role of the gH/gL heterodimer in herpesvirus entry. PMID: 23107819
A Neutralizing Antibody Targeting gH Provides Potent Protection against EBV Challenge In Vivo. PMID: 35348362
Epstein-Barr Virus gH/gL and Kaposi's Sarcoma-Associated Herpesvirus gH/gL Bind to Different Sites on EphA2 To Trigger Fusion. PMID: 32847853
In silico analysis of antiviral phytochemicals efficacy against Epstein-Barr virus glycoprotein H. PMID: 33438528
Epstein-Barr virus glycoprotein gH/gL antibodies complement IgA-viral capsid antigen for diagnosis of nasopharyngeal carcinoma. PMID: 27093005
Integrins as Herpesvirus Receptors and Mediators of the Host Signalosome. PMID: 27501260
Soluble Epstein-Barr virus glycoproteins gH, gL, and gp42 form a 1:1:1 stable complex that acts like soluble gp42 in B-cell fusion but not in epithelial cell fusion. PMID: 16973550
The Cytoplasmic Tail Domain of Epstein-Barr Virus gH Regulates Membrane Fusion Activity through Altering gH Binding to gp42 and Epithelial Cell Attachment. PMID: 27935841
Structural and Mechanistic Insights into the Tropism of Epstein-Barr Virus. PMID: 27094060
Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses. PMID: 17622614
The large groove found in the gH/gL structure is an important functional domain for Epstein-Barr virus fusion. PMID: 23325693
Comparative Mutagenesis of Pseudorabies Virus and Epstein-Barr Virus gH Identifies a Structural Determinant within Domain III of gH Required for Surface Expression and Entry Function. PMID: 26656711
A soluble form of Epstein-Barr virus gH/gL inhibits EBV-induced membrane fusion and does not function in fusion. PMID: 23200314
Crystal structure of the Epstein-Barr virus (EBV) glycoprotein H/glycoprotein L (gH/gL) complex. PMID: 21149717
Epstein-Barr virus gH is essential for penetration of B cells but also plays a role in attachment of virus to epithelial cells. PMID: 10864642
Mapping regions of Epstein-Barr virus (EBV) glycoprotein B (gB) important for fusion function with gH/gL. PMID: 21376360
The KGD motif of Epstein-Barr virus gH/gL is bifunctional, orchestrating infection of B cells and epithelial cells. PMID: 22215569
Human MHC-II with Shared Epitope Motifs Are Optimal Epstein-Barr Virus Glycoprotein 42 Ligands-Relation to Rheumatoid Arthritis. PMID: 29361739
The conserved disulfide bond within domain II of Epstein-Barr virus gH has divergent roles in membrane fusion with epithelial cells and B cells. PMID: 25231307
Molecular basis of EphA2 recognition by gHgL from gammaherpesviruses. PMID: 33235207
Potential entry receptors for human γ-herpesvirus into epithelial cells: A plausible therapeutic target for viral infections. PMID: 34800753
Rabbits immunized with Epstein-Barr virus gH/gL or gB recombinant proteins elicit higher serum virus neutralizing activity than gp350. PMID: 27291087
Mapping the N-terminal residues of Epstein-Barr virus gp42 that bind gH/gL by using fluorescence polarization and cell-based fusion assays. PMID: 20668073
The amino terminus of Epstein-Barr virus glycoprotein gH is important for fusion with epithelial and B cells. PMID: 16160168
The Epstein-Barr virus (EBV) BZLF2 gene product associates with the gH and gL homologs of EBV and carries an epitope critical to infection of B cells but not of epithelial cells. PMID: 7539502
Novel Epstein-Barr virus-like particles incorporating gH/gL-EBNA1 or gB-LMP2 induce high neutralizing antibody titers and EBV-specific T-cell responses in immunized mice. PMID: 27926486
Antibody Generation and Immunogenicity Analysis of EBV gp42 N-Terminal Region. PMID: 34960650
The Murid Herpesvirus-4 gL regulates an entry-associated conformation change in gH. PMID: 18665235
Maternal Epstein-Barr Virus-Specific Antibodies and Risk of Infection in Ugandan Infants. PMID: 33095855
Inhibition of EBV-mediated membrane fusion by anti-gHgL antibodies. PMID: 28939750
Epstein-Barr virus uses different complexes of glycoproteins gH and gL to infect B lymphocytes and epithelial cells. PMID: 9621012
Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV). PMID: 33657166
Mutations of Epstein-Barr virus gH that are differentially able to support fusion with B cells or epithelial cells. PMID: 16103144
Point mutations in EBV gH that abrogate or differentially affect B cell and epithelial cell fusion. PMID: 17307213
Binding-site interactions between Epstein-Barr virus fusion proteins gp42 and gH/gL reveal a peptide that inhibits both epithelial and B-cell membrane fusion. PMID: 17581996
Epstein-Barr virus evasion of CD8(+) and CD4(+) T cell immunity via concerted actions of multiple gene products. PMID: 18977445
The structural basis of herpesvirus entry., PMID:33087881
Scanning Mutagenesis of Human Cytomegalovirus Glycoprotein gH/gL., PMID:26656708
For research use only. Not for human or drug use.
