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Anti-ME2 Polyclonal Antibody

Catalog #:   PHD56901 Specific References (42) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P23368
Overview

Catalog No.

PHD56901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ME2 (Gly220-Ala426).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ME2,NAD-dependent malic enzyme,mitochondrial,Malic enzyme 2,NAD-ME

Purification

Purified by antigen affinity column.

Accession

P23368

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ME2 antibody (PHD56901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

Age, Sex, Metabolic and Pharmacologic Factors May Predict Nonresponse Status to Rheumatoid Arthritis Therapies., PMID:37652478

Inhibition of HIF-1α-AQP4 axis ameliorates brain edema and neurological functional deficits in a rat controlled cortical injury (CCI) model., PMID:35177771

Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan., PMID:34014930

Dominant atopy risk mutations identified by mouse forward genetic analysis., PMID:32810290

Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies., PMID:30244833

Catalase activity of IgG antibodies from the sera of healthy donors and patients with schizophrenia., PMID:28945759

2-Methoxyestradiol inhibits the proliferation and migration and reduces the radioresistance of nasopharyngeal carcinoma CNE-2 stem cells via NF-κB/HIF-1 signaling pathway inactivation and EMT reversal., PMID:28000883

Systemic lupus erythematosus: molecular cloning and analysis of recombinant monoclonal kappa light chain NGTA1-Me-pro with two metalloprotease active centers., PMID:27782255

High levels of homocysteine downregulate apolipoprotein E expression via nuclear factor kappa B., PMID:26981206

IgG abzymes with peroxidase and oxidoreductase activities from the sera of healthy humans., PMID:25946706

Generation and efficacy evaluation of recombinant classical swine fever virus E2 glycoprotein expressed in stable transgenic mammalian cell line., PMID:25198669

Changes in histone H3 lysine 36 methylation in porcine oocytes and preimplantation embryos., PMID:24927323

Molecular profiling of acute and chronic rejections of renal allografts., PMID:24302958

Global histone H3 lysine 27 (H3K27) methylation levels and their prognostic relevance in renal cell carcinoma., PMID:21810159

Additive growth inhibition after combined treatment of 2-methoxyestradiol and conventional chemotherapeutic agents in human pancreatic cancer cells., PMID:21115915

[Preparation and bioactivity of anti-human red blood cell ScFv and CSFV E2 bifunctional fusion protein]., PMID:20353089

Estradiol-17beta and its cytochrome P450- and catechol-O-methyltransferase-derived metabolites stimulate proliferation in uterine artery endothelial cells: role of estrogen receptor-alpha versus estrogen receptor-beta., PMID:20212268

Epigenetic modifications and related mRNA expression during bovine oocyte in vitro maturation., PMID:19567217

Oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats are better activated by combinations of different metal ions., PMID:18837443

Androgen inhibits the growth of carcinoma cell lines established from prostate cancer xenografts that escape androgen treatment., PMID:18550362

Activation of DNA-hydrolyzing antibodies from the sera of autoimmune-prone MRL-lpr/lpr mice by different metal ions., PMID:17561457

HIF-1alpha induced-VEGF overexpression in bone marrow stem cells protects cardiomyocytes against ischemia., PMID:17498737

Bacteriophage T4 nanoparticle capsid surface SOC and HOC bipartite display with enhanced classical swine fever virus immunogenicity: a powerful immunological approach., PMID:17081627

Trimegestone differentially modulates the expression of matrix metalloproteinases in the endometrial stromal cell., PMID:16556677

Metal ions-dependent peroxidase and oxidoreductase activities of polyclonal IgGs from the sera of Wistar rats., PMID:16416456

Metal-dependent hydrolysis of myelin basic protein by IgGs from the sera of patients with multiple sclerosis., PMID:16310860

Evaluation of immobilized metal membrane affinity chromatography for purification of an immunoglobulin G1 monoclonal antibody., PMID:15664358

2-Methoxyestradiol exhibits a biphasic effect on VEGF-A in tumor cells and upregulation is mediated through ER-alpha: a possible signaling pathway associated with the impact of 2-ME2 on proliferative cells., PMID:14670179

Oxytocin innervation of caudal brainstem nuclei activated by cholecystokinin., PMID:14642828

The C-terminal tail of Arabidopsis 14-3-3omega functions as an autoinhibitor and may contain a tenth alpha-helix., PMID:12753586

Development of an orally infectious Sindbis virus transducing system that efficiently disseminates and expresses green fluorescent protein in Aedes aegypti., PMID:12653932

Physical and transcriptional map of a 311-kb segment of chromosome 18q21, a candidate lung tumor suppressor locus., PMID:11401430

Biosynthesis of ribosome-inactivating proteins from callus and cell suspension cultures of Mirabilis expansa (Ruiz &Pavon)., PMID:30754835

The effects of histone acetylation on estrogen responsiveness in MCF-7 cells., PMID:10709763

A comparison of immunohistochemical staining of human cultured mesothelial cells and ovarian tumour cells using epithelial and mesothelial cell markers., PMID:8888953

[Interaction of proteins with platinum and palladium compounds with differing biological activities]., PMID:9273732

Role of the COOH-terminal acidic region of A1 subunit in A2 subunit retention in human factor VIIIa., PMID:8349670

An autologous T cell clone overcomes intra-melanoma heterogeneity for susceptibility to cell-mediated lysis by using multiple lytic mechanisms: in vitro and in vivo analysis., PMID:1841713

Human factor VIIIa subunit structure. Reconstruction of factor VIIIa from the isolated A1/A3-C1-C2 dimer and A2 subunit., PMID:1902833

Allergens of the house dust mite Dermatophagoides farinae. II. Immunological characterization of four allergenic molecules., PMID:2496039

Monoclonal antibodies against mesothelial membrane antigen discriminate between malignant mesothelioma and lung adenocarcinoma., PMID:3276635

Latent forms of type-1 protein phosphatase in rabbit skeletal muscle., PMID:2825677

Datasheet

Document Download

Anti-ME2 Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-ME2 Polyclonal Antibody [PHD56901]
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