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Anti-NCF2 Polyclonal Antibody

Catalog #:   PHD36201 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P19878
Overview

Catalog No.

PHD36201

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NCF2 (Lys355-Val526).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Neutrophil NADPH oxidase factor 2,NOXA2,p67-phox,NADPH oxidase activator 2,67 kDa neutrophil oxidase factor,Neutrophil cytosol factor 2,NCF-2,NCF2,P67PHOX

Purification

Purified by antigen affinity column.

Accession

P19878

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NCF2 antibody (PHD36201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Immunocytochemistry/ Immunofluorescence
    NCF2 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with NCF2 (PHD36201) at 5 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Transcriptomic signatures in peripheral CD4+T-lymphocytes may reflect melanoma staging and immunotherapy responsiveness prior to ICI initiation., PMID:40226614

Revealing disease subtypes and heterogeneity in common variable immunodeficiency through transcriptomic analysis., PMID:39396099

Targeted serum proteome profiling reveals nicotinamide adenine dinucleotide phosphate (NADPH)-related biomarkers to discriminate linear IgA bullous disorder from dermatitis herpetiformis., PMID:38908771

Recent topics and advanced therapies in chronic granulomatous disease., PMID:36534309

The diagnostic potential of novel biomarkers of hypertension in men., PMID:36457990

Bisphenol A: Potential Factor of Miscarriage in Women in the Context of the Phenomenon of Neutrophil Extracellular Traps., PMID:36181646

Utility of Immunohistochemistry and Immunofluorescence in Determining the Pathogenic Variants of Chronic Granulomatous Disease., PMID:34651207

A novel mutation in NCF2 resulting in very-early-onset colitis and juvenile idiopathic arthritis in a patient with chronic granulomatous disease., PMID:31832070

Evidence for a direct link between PAD4-mediated citrullination and the oxidative burst in human neutrophils., PMID:30323221

Role of Flow Cytometry in the Diagnosis of Chronic Granulomatous Disease: the Egyptian Experience., PMID:27222152

Regulation of NADPH oxidase gene expression with PKA and cytokine IL-4 in neurons and microglia., PMID:22565378

Association of NCF2, IKZF1, IRF8, IFIH1, and TYK2 with systemic lupus erythematosus., PMID:22046141

The association between single-nucleotide polymorphisms of NCF2 and systemic lupus erythematosus in Chinese mainland population., PMID:20842512

Surfing a genetic association interaction network to identify modulators of antibody response to smallpox vaccine., PMID:20613780

Expression of Th2-skewed pathology mediators in monocyte-derived type 2 of dendritic cells (DC2)., PMID:19643136

Binding of pleomorphic adenoma gene-like 2 to the tumor necrosis factor (TNF)-alpha-responsive region of the NCF2 promoter regulates p67(phox) expression and NADPH oxidase activity., PMID:17462995

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-NCF2 Polyclonal Antibody [PHD36201]
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