Catalog No.
YXX10001
Expression system
E. coli
Species
Escherichia coli
Protein length
Met1-Lys321
Predicted molecular weight
36.34 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P06709
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Bifunctional ligase/repressor BirA, Biotin operon repressor, Biotin--[acetyl-CoA-carboxylase] ligase, 6.3.4.15, Biotin--protein ligase, Biotin-[acetyl-CoA carboxylase] synthetase, birA, bioR, dhbB, b3973, JW3941
Identification of E3 Ubiquitin Ligase Substrates Using Biotin Ligase-Based Proximity Labeling Approaches., PMID:40299435
The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in Shigella flexneri., PMID:39480076
Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex., PMID:39271111
[Progress in research and application of the biotin ligase BirA and its mutants in pathogen-host interaction]., PMID:39044570
Comparing the BAD Protein Interactomes in 2D and 3D Cell Culture Using Proximity Labeling., PMID:38959414
A one-process production of completely biotinylated proteins in a T7 expression system., PMID:38770738
Construction of a grpE-based plasmid addiction system in Escherichia coli and its application in phloroglucinol biosynthesis., PMID:38724452
Molecular recording of calcium signals via calcium-dependent proximity labeling., PMID:38658655
Chloroflexus aurantiacus acetyl-CoA carboxylase evolves fused biotin carboxylase and biotin carboxyl carrier protein to complete carboxylation activity., PMID:38572988
One-step production of fully biotinylated and glycosylated human Fc gamma receptors., PMID:37734055
Molecular methods to study protein trafficking between organs., PMID:36478633
AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants., PMID:36190279
Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells., PMID:35788163
A simple method for labeling proteins and antibodies with biotin using the proximity biotinylation enzyme TurboID., PMID:35030423
Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions., PMID:34493720
Shifting proteomes: limitations in using the BioID proximity labeling system to study SNARE protein trafficking during infection with intracellular pathogens., PMID:34323972
Proteomics of protein trafficking by in vivo tissue-specific labeling., PMID:33888706
Reactive intermediates for interactome mapping., PMID:33458734
Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method., PMID:33421001
BirA*-protein A fusion protein based BioEnhancer amplifies western blot immunosignal., PMID:33354816
Proximity-Dependent Biotinylation (BioID) of Integrin Interaction Partners., PMID:33215377
Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays., PMID:33023547
Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model Systems., PMID:32843435
Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions., PMID:32833213
The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection., PMID:32817212
Rapid Fabrication of Protein Microarrays via Autogeneration and on-Chip Purification of Biotinylated Probes., PMID:32810400
Locus-Specific Chromatin Proteome Revealed by Mass Spectrometry-Based CasID., PMID:32681487
Biotin Synthesis in Ralstonia eutropha H16 Utilizes Pimeloyl Coenzyme A and Can Be Regulated by the Amount of Acceptor Protein., PMID:32680858
Deconstructing sarcomeric structure-function relations in titin-BioID knock-in mice., PMID:32561764
Biotin Proximity Labeling to Identify Protein-Protein Interactions for Cavin1., PMID:32548822
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks., PMID:32478742
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions., PMID:32391793
A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells., PMID:32321993
Two chromatographic schemes for protein purification involving the biotin/avidin interaction under native conditions., PMID:32268955
Mapping p38α mitogen-activated protein kinase signaling by proximity-dependent labeling., PMID:32189389
Biotinylation of Membrane Proteins for Binder Selections., PMID:32112321
In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation., PMID:31963153
Tuning Allostery through Integration of Disorder to Order with a Residue Network., PMID:31899864
A simple method for non-denaturing purification of biotin-tagged proteins through competitive elution with free biotin., PMID:31825236
Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation., PMID:31658274
Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes., PMID:31633696
Protein-Protein Interaction Mapping by 2C-BioID., PMID:31483108
Identifying Synaptic Proteins by In Vivo BioID from Mouse Brain., PMID:31124092
Exploring a Tumor-Intrinsic PD-L1 Signal with Proximity-Dependent Biotin Identification in Lung Cancer Cells., PMID:31021072
Proteomic navigation using proximity-labeling., PMID:30953756
Tetraspanin-decorated extracellular vesicle-mimetics as a novel adaptable reference material., PMID:30863514
A bacterial display system for effective selection of protein-biotin ligase BirA variants with novel peptide specificity., PMID:30858523
Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus., PMID:30808984
Calpain Purification Through Calpastatin and Calcium: Strategy and Procedures., PMID:30710277
Localized protein biotinylation at DNA damage sites identifies ZPET, a repressor of homologous recombination., PMID:30567999
For research use only. Not for human or drug use.
